Detection and identification ofPodophyllotoxin produced by cell cultures derived from podophyllum hexandrum royle

Uden, W van; Pras, Niesko; Visser, Joost; Malingré, Theo M.

doi:10.1007/bf00716832

Cited by 100 publications

(42 citation statements)

References 16 publications

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“…We compared the effectiveness of the different reported methods for the extraction of PTOX from plant material (Canel et al, 2001;Van Uden et al, 1989;Zhang et al, 2007;Baldi et al, 2008;Chattopadhyay et al, 2004), applying them to Linum cell cultures. As can be seen in Figure 3, the best results were obtained by using methanol/dichloromethane, followed by the buffer method.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of podophyllotoxin extraction method fromLinum albumcell cultures

Yousefzadi

Sharifi

Chashmi

et al. 2010

Pharmaceutical Biology

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Section: Resultsmentioning

confidence: 99%

“…The dichloromethane fractions were then collected, dried and dissolved in 1 mL of HPLC grade methanol and then injected into the HPLC (Van Uden et al, 1989).…”

Section: Methods 2: Buffer Extractionmentioning

confidence: 99%

Optimization of podophyllotoxin extraction method fromLinum albumcell cultures

Yousefzadi

Sharifi

Chashmi

et al. 2010

Pharmaceutical Biology

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“…However wall growth was more pronounced during the later stages of the fermentation. Dry c eil weight (12,13). Propagation of P. hexandrum cells at the callus level, however, would not be able to meet the demand of the drug.…”

Section: Case Study Conessinementioning

confidence: 98%

Bioprocessing Engineering Considerations in the Production of Secondary Metabolites by Plant Cell Suspension Cultures

Srivastava

2003

New Horizons in Biotechnology

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Plant cell cultivation technique serves as an alternative source of a variety of chemicals of diverse nature. However, major bottlenecks in large-scale cultivation of plant cells are low growth rate, shear sensitivity due to their fragile nature, adequate mixing, oxygen transfer and maintenance of aseptic conditions. To complicate the situation further, plant cell products are intracellular and concentrations are generally very low. These problems have been addressed to some extent by optimizing biological and/or technological parameters. Biological parameters include proper selection of medium composition with respect to its C and N source CIN ratio, phosphate ions, and precursor and plant growth regulators.Also light temperature and aeration can be altered to improve yield. The major technological parameter is mixing. Adequate mixing and homogenous conditions in reactor are particularly difficult to achieve due to shear sensitive nature of plant cells. The problem is further compounded when at high cell concentration the changes in fluid rheology result in Non Newtonian behaviour leading to clumping of cells and enhanced sedimentation. This phenomenon restricts effective mass and heat transfer inside cell clumps and therefore has an unpredictive effect on growth and product formation. Plant cells therefore, require balanced aeration as high aeration may remove CO2 (possible nutrient) from culture broth. Another major challenge in growth of plant cells is the sticky nature of late exponential phase cells due to excretion of polysaccharides. This results in problems relating to nutrient and oxygen diffusion to cello Optimum concentration of calcium ions &Ior addition of pectinase/cellulase could be used to minimize clumping to the desirable level.There are several cases where plant cell culture has produced high amounts of secondary metabolites than the whole plant. One such example of our laboratory is Holarrhina antidysentrica, which produces antidysentric drug, conessine. Modification of MS medium by addition of growth factors, sucrose and optimal inoculum resulted in increased yield of the alkaloid. The same suspension culture was propagated in the bioreactor with precursor, cholesterol feeding, which led to 160 times higher productivity than that of the native plant. Salient features of this 173

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“…Refractory testicular lymph and myeloid leukemia and leukemia in stomach, ovarian, brain, breast, pancreatic, and large-cell lung cancers can all be cured by using etoposide, combined with other drugs. Teniposide is less beneficial than etopside but still used for the treatment of lymphoblasts [7,33]. The other uses of this plant in pharmacology and therapeutics are mentioned below and they are based on constituents different from those mentioned above.…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

“…Some scientists prepared the derivatives of podophyllotoxin and then noticed that these substances were pharmacologically active in case of cytotoxic activity at micromolecular level [55][56][57]. Uden et al studied the phenylpropanoid and said that it is a derived lignan of podophyllotoxin [33]. Then it is used as first line raw compound for the chemical synthesis of the antitumour agents like etoposide and teniposide.…”

Section: Cytotoxic Activitymentioning

confidence: 99%