Detection and localization of antibody ingested with a mosquito bloodmeal

Hatfield, Paul

doi:10.1111/j.1365-2915.1988.tb00206.x

Cited by 37 publications

(23 citation statements)

References 21 publications

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“…Sera from mice bitten by Ae. aegypti localized similarly ( Hatfield 1988b), while sera from rabbits exposed to Ae. communis bites identified a 21.5‐kDa salivary antigen in whole body or SG extracts ( Brummer‐Korvenkontio et al .…”

Section: Discussionmentioning

confidence: 99%

Induced immunity against the mosquito Anopheles stephensi: reactivity characteristics of immune sera

Almeida¹,

Billingsley

1999

Medical Vet Entomology

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This study shows the progression of immune responses in mice during five sequential immunizations with Anopheles stephensi mosquito extracts, characterized by ELISA, Western blot and immunohistochemistry. When exposed repeatedly to mosquito bites, control mice developed antibodies which reached titres of 1:10(5), reacted weakly in Western blot analysis and were localized to the salivary glands. Mice immunized with mosquito head plus salivary glands, midgut, ovary, fat body, midgut microvilli (Mv) and midgut basolateral plasma membrane (Blm), showed increased titre with each successive boost. Epitopes were shared between sera or were specific to the immunizing or heterologous extract. Anti-Mv and Blm sera recognized proteins labelled by anti-midgut serum and gave specific reactions with the midgut and head. Cross-reactivity was confirmed immunohistochemically.

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Section: Discussionmentioning

confidence: 99%

Induced immunity against the mosquito Anopheles stephensi: reactivity characteristics of immune sera

Almeida¹,

Billingsley

1999

Medical Vet Entomology

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“…Both of these research groups showed that the entry of peroxidase into epithelial cells was achieved directly through the microvilli, not through pinocytotic invaginations, and was accomplished by diffusion and progressive spreading throughout the cells. In addition, IgG passed through midgut epithelial cells (Vaughan and Azad, 1988;Hatfield, 1988) and specifically bound to ovarian tissues (Ramasamy et al, 1988) of the mosquito species by a receptor-mediated mechanism. In our studies, GFP associated with cellular membranes in the gut, hemolymph, and fat body, but was not found intracellularly in the midgut epithelial cells.…”

Section: June 2002mentioning

confidence: 99%

Uptake, flow, and digestion of casein and green fluorescent protein in the digestive system of Lygus hesperus Knight

Habibi

Brandt

Coudron

et al. 2002

Arch Insect Biochem Physiol

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Selected compounds were used to study physiological processes associated with digestion in the western tarnished plant bug, Lygus hesperus Knight. Durations of passage and rates of absorption, digestion, and excretion were determined for a digestible protein (casein), a non-digestible protein (green fluorescent protein, GFP), and a non-digestible carbohydrate (dextran). Dextran was used as a control to monitor the non-absorptive flow rate of ingesta through the digestive system. Fluorescent tracking of FITC-conjugates of casein and dextran, as well as immunoblotting and immunofluorescent staining of casein and GFP, were used to monitor the degradation (in vitro) and ingestion, digestion, and distribution (in vivo) of the respective compounds. Under our experimental conditions, L. hesperus took discrete meals, feeding and excreting at 2-3 h intervals. Rate of food passage was variable. FITC-dextran was found in the fecal material of most insects by 6-8 h after treatment initiation; by 12 h, 95% of ingested FITC-dextran was recovered from all insects. FITC-casein was digested extensively in in vitro homogenates of gut, hemolymph, and salivary gland. In vivo, FITC-casein was ingested and partially absorbed as a holoprotein into the hemolymph. Ingested FITC-casein was partially degraded in the gut and hemolymph within 2 h of ingestion, and no holoprotein was found after 12 h. In contrast, there was no detectable degradation of GFP in hemolymph, gut, and salivary gland homogenates after 24 h of incubation. Ingested GFP was not degraded in gut or hemolymph up to 8 h after treatment initiation, but did transfer to the hemolymph as a holoprotein. Analysis of immunohistological images confirmed that GFP bound to gut epithelial cell brush-border membranes. However, the mechanism by which GFP and casein pass as holoproteins into the hemolymph remains unknown.

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“…Many studies with other insects have shown the uptake of proteins from the diet into the haemolymph (e.g. immunoglobulin G: Hatfield, 1988;horseradish peroxidase: Fishman and Zlotkin, 1984), probably intercellularly through leaky epithelial cell gap junctions (Billingsley and Lehane, 1996). Vaughan et al (1990) calculated that 0.5% of ingested immunoglobulin passed into the haemolymph in mosquitoes.…”

Section: Introductionmentioning

confidence: 99%

Development of hypopharyngeal glands in adult honey bees fed with a Bt toxin, a biotin-binding protein and a protease inhibitor

et al. 2004

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-To investigate potential impacts of insecticidal transgene products on hypopharyngeal gland development, newly-emerged adult honey bees were kept in cages and fed for ten days with a pollen-food to which one of the following proteins had been added at concentrations equivalent to pollen expressing 1.25% (of total protein) aprotinin, 11.2 µM avidin or 0.3% Cry1Ba protein. Control bees were fed pollenfood without additive, with casein added, or pollen-candy. None of the treatments significantly affected bee survival, or the mean diameters of gland acini, gland mass or protein content on Days 1, 4, 8 or 10. Bees consumed significantly more of the pollen-candy than the other foods. Assays showed no evidence of aprotinin, avidin or Cry1Ba in the glands of bees fed with these proteins. Thus effects of these transgene products on gland development are unlikely, but field trials using transgenic plants are required for a full assessment.Apis mellifera / hypopharyngeal gland / insecticidal transgenic plant protein / laboratory assay

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Detection and localization of antibody ingested with a mosquito bloodmeal

Cited by 37 publications

References 21 publications

Induced immunity against the mosquito Anopheles stephensi: reactivity characteristics of immune sera

Induced immunity against the mosquito Anopheles stephensi: reactivity characteristics of immune sera

Uptake, flow, and digestion of casein and green fluorescent protein in the digestive system of Lygus hesperus Knight

Development of hypopharyngeal glands in adult honey bees fed with a Bt toxin, a biotin-binding protein and a protease inhibitor

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