Detection and molecular analysis of Pseudorabies virus strains isolated from dogs and a wild boar in Italy

Moreno, Ana; Sozzi, Enrica; Grilli, G.; Gibelli, Lucia; Gelmetti, Daniela; Lelli, Davide; Chiari, Mario; Prati, Paola; Alborali, G. L.; Boniotti, Maria Beatrice; Llombart‐Bosch, Antonio; Cordioli, Paolo

doi:10.1016/j.vetmic.2015.04.001

Cited by 53 publications

(75 citation statements)

References 23 publications

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“…Besides this variability dependent on geographical location, it appears that strains may evolve differently according to their host (i.e., domestic swine or wild boars) [12]. Italian strains of clade A could be classified into three groups, for example: "Italian clade 1" containing all the strains isolated from wild boars and hunting dogs, and "Italian clade 2" and "Italian clade 3", containing strains isolated from domestic pigs or epidemiologically-related dogs, the most recent strains belonging to clade 2 [13].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

et al. 2019

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Pseudorabies (PR), also known as Aujeszky’s disease, is an economically important disease for the pig industry. It has been eradicated in domestic pigs in many European countries, including France, but its causative agent—Suid Herpesvirus 1—is still circulating in wild boars. The risk of endemic PR in wild fauna lies in reintroducing the virus among domestic pigs and transmitting it to other mammals, especially hunting dogs for which the disease is rapidly fatal. As such infections are regularly reported in France, this study genetically characterized canine PR virus strains in the country to obtain information on their diversity and evolution. Partial sequencing of the glycoprotein C-encoding gene from 55 virus strains isolated from dogs between 2006 and 2018 showed that 14 strains belonged to genotype I-clade A and another 38 to genotype I-clade B, two clades usually reported in Western Europe. More surprisingly, three strains were found to belong to genotype II, suggesting an Asian origin. Genotype I-clade A strains exhibited the highest diversity as five geographically segregated genogroups were identified.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

et al. 2019

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“…The virus primarily affects pigs but it is also known to occasionally occur in cattle, sheep, goats, dogs, cats and wild boars which constitute a reservoir of the virus. Aujeszky's disease is a contagious infection and is mainly transmitted by direct and indirect contact between pigs [3,4].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

et al. 2016

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The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identifi ed in this study showed high similarity and signifi cant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and signifi cant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identifi ed in swine in Republic of Montenegro. Besides that, these results confi rmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.

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“…Previous studies demonstrated that the Italian SHV-1 strains are divided into three clusters (4) and that clear genetic distinction between strains isolated from hunting dogs exposed to wild boars and those originated from working dogs, with domestic pigs as likely sources, does exist (5). By phylogenetic analysis of partial gC gene sequences, ADV32751/Italy2014 apparently belongs to the Italian cluster 1 with other SHV-1 strains isolated from wild boars and hunting dogs in Italy (4). To date, as far as we know, this is the ﬁrst whole-genome sequence of an SHV-1 strain isolated in Italy.…”

Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Sequence of a Suid Herpesvirus-1 Strain Isolated from the Brain of a Hunting Dog in Italy

et al. 2016

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Detection and molecular analysis of Pseudorabies virus strains isolated from dogs and a wild boar in Italy

Cited by 53 publications

References 23 publications

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

Whole-Genome Sequence of a Suid Herpesvirus-1 Strain Isolated from the Brain of a Hunting Dog in Italy

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