Detection and Phylogenetic Analysis of a Novel Tick-Borne Virus in Yunnan and Guizhou Provinces, Southwestern China

Wang, Anan; Pang, Zengyuan; Liu, Lin; Ma, Qianwen; Han, Yize; Guan, Zhen; Qin, Hao; Niu, Guoyu

doi:10.3390/pathogens10091143

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…Geographically, the minimum infection rate (MIR) of DBTV in ticks from Dandong city, Fengcheng city, Tieling city, and Benxi city, was 9.22% (19/206, 95% CI = 0.0579–0.1423), 6.67% (11/165, 95%CI = 0.0355–0.1192), 10% (10/100, 95%CI = 0.516–0.1804), 12.22% (11/90, 95%CI = 0.0655–0.2122), respectively ( Table 1 ). The MIR of DBTV in this research was markedly higher than in previous surveys in the Shandong, Yunnan, and Guizhou provinces of China ( Shao et al, 2020 ; Wang et al, 2021 ). This work presented positive for Severe fever with thrombocytopenia syndrome virus (SFTSV) in only four tick nucleic acid pools from Dandong City, with a total MIR of 0.71% (4/561, 95% CI = 0.0023–0.0194), which was compared to the MIR reported for SFTSV in Shandong Province which was slightly lower than previous studies ( Shao et al, 2020 ).…”

Section: Resultscontrasting

confidence: 85%

“…The RdRp phylogenetic analysis indicated that DBTV clustered closely with the Yongjia tick virus, Uukuniemi virus, and with a group of Tacheng tick viruses 2 (TcTV2) in the Uukuvirus genus, forming a separate branch ( Figure 2B ). Of these, TcTV2 is responsible for severe disease, and numerous studies have identified DBTV with such an evolutionary signature, further suggesting that DBTV may emerge as a human pathogen ( Wang et al, 2021 ; Yang et al, 2021 ; He et al, 2022 ). Furthermore, in this research, the seven DBTV strains displayed a high degree of amino acid identity with each other, despite being the same tick species from different eastern border regions of Liaoning Province.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the diversity of tick-borne viruses at the border areas in Liaoning Province, China

et al. 2023

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Ticks play a significant role in transmitting arboviruses, which pose a risk to human and animal health. The region of Liaoning Province, China, with abundant plant resources with multiple tick populations, has reported several tick-borne diseases. However, there remains a scarcity of research on the composition and evolution of the tick virome. In this study, we conducted the metagenomic analysis of 561 ticks in the border area of Liaoning Province in China and identified viruses related to known diseases in humans and animals, including severe fever with thrombocytopenia syndrome virus (SFTSV) and nairobi sheep disease virus (NSDV). Moreover, the groups of tick viruses were also closely related to the families of Flaviviridae, Parvoviridae, Phenuiviridae, and Rhabdoviridae. Notably, the Dabieshan tick virus (DBTV) of the family Phenuiviridae was prevalent in these ticks, with the minimum infection rate (MIR) of 9.09%, higher than previously reported in numerous provinces in China. In addition, sequences of tick-borne viruses of the family Rhabdoviridae have first been reported from the border area of Liaoning Province, China, after being described from Hubei Province, China. This research furthered the insight into pathogens carried by ticks in the northeastern border areas of China, offering epidemiological information for possible forthcoming outbreaks of infectious diseases. Meanwhile, we provided an essential reference for assessing the risk of tick bite infection in humans and animals, as well as for exploring into the evolution of the virus and the mechanisms of species transmission.

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Section: Resultscontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Analysis of the diversity of tick-borne viruses at the border areas in Liaoning Province, China

et al. 2023

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“…It exists in several provinces in southern China. DTV genetically clustered into a branch of the Yongjia tick virus ( Li et al, 2015 ; Shao et al, 2020 ; Zhu et al, 2020 ; Wang A et al, 2021 ; Ma et al, 2022 ). However, there was no evidence of DTV infection in humans.…”

Section: Resultsmentioning

confidence: 99%

Diversity of species and geographic distribution of tick-borne viruses in China

Wu,

Zhou,

Mao

et al. 2024

Front. Microbiol.

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IntroductionTick-borne pathogens especially viruses are continuously appearing worldwide, which have caused severe public health threats. Understanding the species, distribution and epidemiological trends of tick-borne viruses (TBVs) is essential for disease surveillance and control.MethodsIn this study, the data on TBVs and the distribution of ticks in China were collected from databases and literature. The geographic distribution of TBVs in China was mapped based on geographic locations of viruses where they were prevalent or they were detected in vector ticks. TBVs sequences were collected from The National Center for Biotechnology Information and used to structure the phylogenetic tree.ResultsEighteen TBVs from eight genera of five families were prevalent in China. Five genera of ticks played an important role in the transmission of TBVs in China. According to phylogenetic analysis, some new viral genotypes, such as the Dabieshan tick virus (DTV) strain detected in Liaoning Province and the JMTV strain detected in Heilongjiang Province existed in China.DiscussionTBVs were widely distributed but the specific ranges of viruses from different families still varied in China. Seven TBVs belonging to the genus Orthonairovirus of the family Nairoviridae such as Nairobi sheep disease virus (NSDV) clustered in the Xinjiang Uygur Autonomous Region (XUAR) and northeastern areas of China. All viruses of the family Phenuiviridae except Severe fever with thrombocytopenia syndrome virus (SFTSV) were novel viruses that appeared in the last few years, such as Guertu virus (GTV) and Tacheng tick virus 2 (TcTV-2). They were mainly distributed in the central plains of China. Jingmen tick virus (JMTV) was distributed in at least fourteen provinces and had been detected in more than ten species of tick such as Rhipicephalus microplus and Haemaphysalis longicornis, which had the widest distribution and the largest number of vector ticks among all TBVs. Parainfluenza virus 5 (PIV5) and Lymphatic choriomeningitis virus (LCMV) were two potential TBVs in Northeast China that could cause serious diseases in humans or animals. Ixodes persulcatus carried the highest number of TBVs, followed by Dermacentor nuttalli and H. longicornis. They could carry as many as ten TBVs. Three strains of Tick-borne encephalitis (TBEV) from Inner Mongolia Province clustered with ones from Russia, Japan and Heilongjiang Province, respectively. Several SFTSV strains from Zhejiang Province clustered with strains from Korea and Japan. Specific surveillance of dominant TBVs should be established in different areas in China.

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“…As of now, the full-length coding genomes of the L and S segments of the DBTV have been published. In recent years, DBTV has been discovered in various regions of China, including Shandong ( Shao et al, 2021 ), Zhejiang ( He et al, 2022 ), Liaoning ( Yang et al, 2021 ), Hubei, Yunnan, and Guizhou Provinces ( Wang et al, 2021 ). Epidemiological investigations have shown that DBTV is widely disseminated in Linyi, Tai’an, Weifang, Yantai ( Ma et al, 2022 ), and Rizhao city of Shandong Province ( Shao et al, 2020 ) and phylogenetic analysis revealed distinct lineages of DBTV ( Ma et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Other tick species such as Rhipicephalus microplus and R. haemaphysaloides , have also been discovered to carry DBTV ( Ma et al, 2022 ). However, researchers have failed to isolate DBTV from positive tick sample ( Wang et al, 2021 ). The available literature shows that research on ticks in DBTV transmission is scarce.…”

Section: Introductionmentioning

confidence: 99%

Discovery and vertical transmission analysis of Dabieshan Tick Virus in Haemaphysalis longicornis ticks from Chengde, China

Xu,

Gao,

et al. 2024

Front. Microbiol.

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IntroductionTicks are important blood-sucking ectoparasites that can transmit various pathogens, posing significant threats to the wellbeing of humans and livestock. Dabieshan tick virus (DBTV) was initially discovered in 2015 in Haemaphysalis longicornis ticks from the Dabieshan mountain region in Hubei Province, China. In recent years, DBTV has been discovered in various regions of China, including Shandong, Zhejiang, Liaoning, Hubei, Yunnan, and Guizhou Provinces. However, the researches on tick-borne transmission of DBTV are scarce.MethodsThis study utilized the small RNA sequencing (sRNA-seq) method to identify tick-associated viruses in ticks collected from Chengde in Hebei Province and Yongcheng in Henan Province, leading to the discovery of a new DBTV strain in Hebei. The complete coding genome of DBTV Hebei strain was obtained through RNA-seq and Sanger sequencing. Furthermore, the transmission experiment of DBTV in H. longicornis was examined in laboratory for the first time.ResultsDBTV was detected in newly molted adult H. longicornis ticks collected in Chengde, Hebei Province. Additionally, DBTV was also detected in both unfed nymphs and engorged females of H. longicornis collected from Chengde, with a positive rate of 20% and 56.25%, respectively. The complete coding genome of DBTV (OP682840 and OP716696) were obtained, and phylogenetic analysis revealed that the DBTV Hebei strain clustered with previously reported DBTV strains. Furthermore, this virus was observed in engorged females, eggs, and larvae of the subsequent generation.DiscussionIt is necessary to expand the scope of DBTV investigation, particularly in northern China. This study demonstrated that DBTV can be transmitted from engorged females to larvae of the next generation. Moreover, the detection of DBTV in unfed nymphs and adults (which moulted from engorged nymphs) collected from the filed of Chengde suggests that H. longicornis serves as a potential transmission host and reservoir for DBTV through transstadial and transovarial transmission. However, there remains a lack of research on the isolation and pathogenicity of DBTV, highlighting the need for further studies to mitigate potential harm to the health of animals and humans.

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Detection and Phylogenetic Analysis of a Novel Tick-Borne Virus in Yunnan and Guizhou Provinces, Southwestern China

Cited by 7 publications

References 16 publications

Analysis of the diversity of tick-borne viruses at the border areas in Liaoning Province, China

Analysis of the diversity of tick-borne viruses at the border areas in Liaoning Province, China

Diversity of species and geographic distribution of tick-borne viruses in China

Discovery and vertical transmission analysis of Dabieshan Tick Virus in Haemaphysalis longicornis ticks from Chengde, China

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