Detection and Quantification of DNA by Fluorophore-Induced Plasmonic Current: A Novel Sensing Approach

Abstract

We report on the detection and quantification of aqueous DNA by a fluorophore-induced plasmonic current (FIPC) sensing method. FIPC is a mechanism described by our group in the literature where a fluorophore in close proximity to a plasmonically active metal nanoparticle film (MNF) is able to couple with it, when in an excited state. This coupling produces enhanced fluorescent intensity from the fluorophore–MNF complex, and if conditions are met, a current is generated in the film that is intrinsically linked to the properties of the fluorophore in the complex. The magnitude of this induced current is related to the spectral properties of the film, the overlap between these film properties and those of the fluorophore, the spacing between the nanoparticles in the film, the excitation wavelength, and the polarization of the excitation source. Recent literature has shown that the FIPC system is ideal for aqueous ion sensing using turn-on fluorescent probes, and in this paper, we subsequently examine if it is possible to detect aqueous DNA also via a turn-on fluorescent probe, as well as other commercially available DNA detection strategies. We report the effects of DNA concentration, probe concentration, and probe characteristics on the development of an FIPC assay for the detection of non-specific DNA in aqueous solutions.