Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection

Abstract

This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including turkey gizzard and muscle tissues, and chicken muscle tissues, commonly consumed in Ogun State, Nigeria. Minced tissues were treated with phosphate buffer adjusted to pH 7 that was cleaned using C18 SPE-column (Supelclean™) cartridge. For the determination of six antibiotic residues including fluoroquinolones, sulfonamides, and macrolides, a solid-phase extraction method was used, followed by extract analysis using high-performance liquid chromatography–diode array detection (HPLC–DAD). The coefficient of determination (R 2 ) for the external standards for all the analytes ranged between 0.963 and 0.999. The limit of detection (LOD) and quantification (LOQ) ranged between 5.37 – 55.4 μg/kg, and 17.9–185 μg/kg, respectively. Enrofloxacin, sulfadimethoxine, sulfamerazine, and tylosin showed high concentration levels in the frozen poultry beyond acceptable maximum residue limits (MRLs). The six drugs considered in this study were present at higher concentrations in domestic chicken tissues than the permissible level. This suggests that farmers do not observe the cessation period before poultry birds previously treated with antibiotics are sold to consumers thus exposing them to potentially hazardous antibiotic residues.

Recent Advances in the Determination of Veterinary Drug Residues in Food

“…These findings highlight the necessity for action in drug administration to minimize excessive residues. The LC-MS method is widely employed for the detection of poultry samples, making it a suitable instrument for this purpose [ 60 ].…”

Comprehensive Screening of Salinomycin in Feed and Its Residues in Poultry Tissues Using Microbial Inhibition Tests Coupled to Enzyme-Linked Immunosorbent Assay (ELISA)

“…These methods are based on the detection of residues of inhibitory substances in a wide range of matrices, while the principle is the inhibition of the growth of the test organism by the action of the antibiotic present in the examined sample. Since in the case of screening it is only a matter of confirming or refuting the presence of inhibitory substances in the sample and thus it is a qualitative or semi-quantitative determination, to confirm the result it is necessary to use confirmatory methods based either on the chromatographic (LC-MS/MS) [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ] or immunological principles (enzyme-linked immunosorbent assay—ELISA) [ 10 , 35 , 36 ].…”

Recent Trends in the Development of Carbon-Based Electrodes Modified with Molecularly Imprinted Polymers for Antibiotic Electroanalysis

“…Therefore, in order to reduce human, animal and ecological health risks due to antibiotic contamination, there is an increasing need for the development of simple, rapid and reliable methods for the straightforward sensitive in situ or ex situ monitoring of these contaminants in various biological, food and environmental samples. Most approaches for the detection of antibiotics are based on different chromatographic techniques either as such [41][42][43][44] or coupled with extraction methods [45][46][47]. Microbiological assays and chromatographic methods applied for antibiotic identification and determination were reviewed in 2020 by Pauter et al [48].…”