Detection in capillary electrophoresis

Swinney, Kelly; Bornhop, Darryl J.

doi:10.1002/(sici)1522-2683(20000401)21:7<1239::aid-elps1239>3.0.co;2-6

Cited by 182 publications

(123 citation statements)

References 93 publications

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“…Introduction Photometric and fluorimetric optical detection methods are frequently used in capillary based separation techniques including capillary electrophoresis (CE) and capillary liquid chromatography (cap-LC) [1][2][3][4][5][6]. While photometric detection is generally valuable and the most common detection in CE and cap-LC, the combination of the most sensitive detection method of laser-induced fluorescence (LIF) detection with CE provides a powerful separation platform with a wide range of advantages including speed, high resolution, efficiency, and sensitivity, as well as low sample and reagent consumption [7][8][9], for applications such as glycomics [10][11][12].…”

Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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Graphical Abstract (please choose)A new generation of integrated miniaturized fibre-coupled solid-state light sources based on microfabricated light emitting diode micro-array (µ-LED), micropackaged with a custom band-pass optical interference filter deposited at the end of an optical fibre, is presented and in this work and demonstrated as excitation light source for fluorescence detection in capillary electrophoresis. ALTERNATIVE IMAGES: Key words:Micro-light emitting diode array; light source; optical fibre; capillary electrophoresis; fluorescence detection; separations. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 *Manuscript (including figures, tables, text graphics and associated captions) Click here to view linked References

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Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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“…1 A wide variety of detection techniques for CE have been proposed by many researchers. 2 Each detection technique has both advantages and disadvantages. For example, a laser-induced fluorescence (LIF) is a very sensitive detection technique, but often requires chemical derivatization, because most chemical species, as they are, are not very strongly fluorescent.…”

Section: Introductionmentioning

confidence: 76%

Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection

et al. 2005

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“…CE can be easily coupled with a wide range of detection techniques. Swinney and Bornhop gave an overview including unconventional CE detectors [139]. MS probably has the best capability for complex and universal detection in portable analytical instrumenta-tion, especially due to its detailed composition analysis and suitability for a variety of sample types.…”

Section: Chemiluminescencementioning

confidence: 99%

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

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Detection in capillary electrophoresis

Cited by 182 publications

References 93 publications

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection

Portable capillary-based (non-chip) capillary electrophoresis

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