Detection of a ''Michaelis'' complex by spin labeling in a model enzyme system

ESR Spectroscopy in Polymer Research

“…The spin-labeling technique was originally applied to biological macromole-cules214, 314,739,820,859,906,1065,1075,1443,1537,1716,1907) and then extended to the synthetic polymers such as: polyethylene351, 1801,1947), poly(methyl vinyl ketone )350), poly(methyl methacrylate)343, 819,820,1996), polystyrene32, 342, 345-349, 573), 11. Application of Stable Free Radicals in Polymer Research poly(vinyl chloride)2217, 2232), poly(4-vinylpyridine )1174}, l ·d 11 1 · 1 710, 1843} pyrro 1 one-a y annne copo ymer , maleic anhydride-dimethyl vinyl ether copolymer 820 }, poly( ethylene oxide) 1902, 1963}, poly(ethylene glycol) 2217,2218,2222,2223,2229,2230,2232,2233), polyamides2217, 2231, 2232), polyurethane 2386}, poly(-y-benzyl glutamate)1429}, polysiloxanes 1l85 ,1963), cellulose and lignin 32 , 1374,2225-2227), and polynuc1eic acids 235 , 2089).…”

Solid‐state ESR differentiation between racemate versus enantiomer

“…Differences between the ESR spectra of diastereomers were also detected in both solid and frozen states. 17 The ESR detection of the formation of cyclodextrin (CD) inclusion complexes with nitroxide radicals by Kaiser et al in 1970 18 gave rise to extensive work on the stereochemical investigation of CD complexes by ESR. Enantioselective complexation of cyclohexa-and cycloheptaamylose with nitroxide esters and subsequent stereoselective catalytic hydrolysis of the esters was detected and investigated by means of ESR spectroscopy by Kaiser et al in 1975.…”

Improving Spin Probe Methodologies to Investigate Supramolecular Assemblies

“…A large set of spin‐labelled macrocyclic hosts have been synthesized and characterized in the last decades . The main advantage of these spin probes consists in the possibility of using EPR spectroscopy for detecting binding event still in the presence of diamagnetic guests.…”