Detection of a novel tick-borne flavivirus and its serological surveillance

Shimoda, Hiroshi; Hayasaka, Daisuke; Yoshii, Kentaro; Yokoyama, Masahiro; Suzuki, Kazuo; Kodera, Yuuji; Takeda, Tsutomu; Mizuno, Junko; Noguchi, Keita; Yonemitsu, Kenzo; Minami, Shohei; Kuwata, Ryusei; Takano, Ai; Maeda, Ken

doi:10.1016/j.ttbdis.2019.03.006

Cited by 13 publications

(3 citation statements)

References 28 publications

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“…However, 111 pools tested positive for unknown mosquito flavivirus. New flaviviruses are constantly being described, and a number of novel insect-specific flaviviruses have been described in the recent years [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Lack of Zika Virus and Other Recognized Flaviviruses among the Mosquito Vectors during and Post the Hajj Mass Gathering

Yezli

Yasir

Yassin

et al. 2021

IJERPH

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Makkah city, Kingdom of Saudi Arabia (KSA), contains many of the world’s mosquito vectors of parasitic and arboviral disease and is the site of the Hajj mass gathering. As such there is a risk of exportation and globalization of vector-borne viruses, including the re-emerging Zika virus (ZIKV). There was international concern regarding the introduction of ZIKV to KSA and potential international spread of the virus following the 2016 Hajj which took place few days after the Rio summer Olympics at the height of the ZIKV pandemic. We aimed to detect flaviviruses, including ZIKV, circulating among mosquito hosts in the city of Makkah during and post the 2016 Hajj pilgrimage. Mosquitos (adults and larvae) were sampled from 15 sites in Makkah city during and post the 2016 Hajj and identified to species by morphological keys. Mosquitos were pooled according to date of collection, location, and species. A Pan-Flaviviruses RT-PCR assay that enables identification of 51 flaviviruses species and three tentative species was used to detect flavivirus RNA directly from mosquito homogenates. Between the 10 September and 6 October 2016, 9412 female mosquitos were collected. Of these, 81.3% were Aedes aegypti, 18.6% were Culex species, and 0.1% were Anopheles species. Of the total 493 mosquito pools generated, 242 (49%) were positive by the Pan-Flaviviruses primer set. Sequence analysis revealed that none of the mosquitos carried a pathogenic flavivirus, including ZIKV, but were infected with a novel insect-specific flavivirus. We found no pathogenic flaviviruses circulating in Makkah city during and post the 2016 Hajj and no evidence of introduction of ZIKV through the pilgrimage. Enhanced vector-borne diseases surveillance, prevention, and control are crucial in KSA especially during international mass gatherings such as the annual Hajj to prevent outbreaks and the spread of viruses with epidemic and pandemic potentials.

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Section: Discussionmentioning

confidence: 99%

Lack of Zika Virus and Other Recognized Flaviviruses among the Mosquito Vectors during and Post the Hajj Mass Gathering

Yezli

Yasir

Yassin

et al. 2021

IJERPH

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“…Ticks, as the primary arthropod vectors of pathogen transmission, can transmit a wide range of arboviruses. It has been shown that a number of arboviruses have a significant impact on public health and can cause a range of symptoms in humans or animals, from mild to severe, and even death, for instance, in the last two decades, severe fever with thrombocytopenia syndrome virus and Alongshan virus in China [1,2], Heartland virus and Bourbon virus in the United States [3,4], Hunter island group virus in Australia [5], Yamaguchi virus, Muko virus and Tarumizu tick virus in Japan [6][7][8], Shibuyunji virus in Zambia [9], Dugbe virus in Ghana [10]. In recent years, with increased attention and the development of next-generation sequencing technology, several novel arboviruses have been identified.…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for potential transovarial transmission of Dabieshan tick virus in Haemaphysalis longicornis from Shandong Province, China

Wang,

Tang,

Pang

et al. 2023

PLoS ONE

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Dabieshan tick virus (DBTV) is a newly identified arbovirus, first detected in Haemaphysalis longicornis collected from Hubei Province in 2015. It has been confirmed that DBTV is widely distributed in Shandong Province, China. However, its entomological and epidemiological features remain to be further explored, particularly the feasibility of transovarial transmission. Our research tries to explain the possibility of transovarial transmission of DBTV from engorged female ticks to their offspring. All engorged female adult ticks were sampled from domestic sheep and allowed to lay eggs and hatch in appropriate laboratory conditions. All engorged ticks, larvae and unhatched eggs were classified into pools for nucleic acid extraction and DBTV RNA detection. According to the results of qRT-PCR, the positive rate of DBTV was 6.25% (8/128) in engorged female ticks, 3.57% (1/28) in eggs and 5% (3/60) in larvae pools, respectively. Phylogenetic analysis indicated that DBTV isolates from larvae were similar to those from maternal ticks with more than 99.5% homology, and DBTV was relatively conservative in evolution. Our findings are the first to provide molecular evidence of potential transovarial transmission of DBTV among H. longicornis. Nonetheless, the transovarial transmission of DBTV in frequency and proportion occurring in nature deserves further investigation.

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“…Besides, antibodies against TBEV were detected in deer and wild rodents in the Tochigi and the Shimane prefectures, and antibodies against the TBEV-serocomplex were also detected in wild boars in wide areas of Japan (the Yamaguchi, Wakayama, Hyogo, Oita, Gifu, Toyama and Chiba prefecture), indicating wide distribution of TBEV all over Japan. 3,6,12 Ever since the first confirmed TBE case in 1993, only four additional cases of TBE were reported from Japan, the last one in 2018, although endemic foci of TBEV were detected in various parts of the country, not only in Hokkaido.…”

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confidence: 99%