Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphis aurita) from Brazil

Guimarães, Andresa; Raimundo, Juliana Macedo; Silva, Aline Tonussi da; Carpintero, Fernanda Modesto; Pires, Jeferson Rocha; Benevenute, Jyan Lucas; Machado, Rosangela Zacarias; André, Marcos Rogério; Baldani, Cristiane Divan

doi:10.1590/s1984-296120180068

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…Although different TBP have been described infecting Didelphis sp. [ 5 , 13 , 14 , 15 , 16 , 19 , 23 , 24 ], information on hemoplasmas and TBP on D. aurita still are scarce. In addition, the impact that these microorganisms may have on the health of affected individuals as well as the possible changes on hematological and biochemical profile remain unknown.…”

Section: Introductionmentioning

confidence: 99%

‘Candidatus Mycoplasma Haemoalbiventris’ and Tick-Borne Pathogens in Black-Eared Opossum (Didelphis aurita) from Southeastern Brazil

et al. 2022

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The black-eared opossum (Didelphis aurita) is a South American synanthropic marsupial. The presence of opossums in domestic spaces is relevant in the One-Health context since they are hosts of pathogens and ectoparasites that may affect the health of domestic animals and humans. In this study, we aim to determine the occurrence of hemoplasmas and selected tick-borne pathogens in free-ranging black-eared opossums, along with their molecular characterization, hematological and biochemical evaluation and factors associated with infection, in the municipality of Viçosa, State of Minas Gerais, southeastern Brazil. Thirty black-eared opossums were trapped between March 2021 and June 2022. Ectoparasites were collected. Hematological and biochemical analyses were performed. DNA from EDTA-blood samples were analyzed by PCR and qPCR assays. By molecular analyses, ‘Candidatus Mycoplasma haemoalbiventris’ was the most prevalent hemoparasite (73.3%), followed by Hepatozoon sp. (22.2%). Significant differences were observed in the number of platelets, and in the concentration of protein and globulins in the animals infected by ‘Ca. M. haemoalbiventris’ when compared with the negative group. This is the first report of ‘Ca. M. haemoalbiventris’ infection in D. aurita.

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Section: Introductionmentioning

confidence: 99%

‘Candidatus Mycoplasma Haemoalbiventris’ and Tick-Borne Pathogens in Black-Eared Opossum (Didelphis aurita) from Southeastern Brazil

et al. 2022

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“…In Brazil, several genotypes of A. phagocytophilum , A. marginale , A. bovis , A. platys , E. canis , E. chafeensis , and potential new species, that are not yet named, have been detected in different species of wild animals, including deer 5 – 11 , wild canids 12 – 14 , wild felids 12 , 15 , 16 , coatis 14 , rodents 14 , 17 – 19 , caititus and peccaries 20 , opossums 21 , 22 , and birds 23 , 24 .…”

Section: Introductionmentioning

confidence: 99%

Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

Calchi

Vultão

Alves

et al. 2020

Sci Rep

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Anaplasmataceae agents are obligatory intracellular Gram-negative α-proteobacteria that are transmitted mostly by arthropod vectors. Although mammals of the Superorder Xenarthra (sloths, anteaters, and armadillos) have been implicated as reservoirs for several zoonotic agents, only few studies have sought to detect Anaplasmataceae agents in this group of mammals. This study aimed to investigate the occurrence and genetic diversity of Anaplasma spp. and Ehrlichia spp. in blood and spleen samples of free-living Xenarthra from four different states in Brazil (São Paulo, Mato Grosso do Sul, Rondônia, and Pará). Nested and conventional PCR screening assays were performed to detect the rrs and dsb genes of Anaplasma spp. and Ehrlichia spp., respectively. The assays were positive in 27.57% (91/330) of the Anaplasma spp. and 24.54% (81/330) of the Ehrlichia spp. Of the 91 positive Anaplasma spp. samples, 56.04% were positive in a conventional PCR assay targeting the 23S–5S intergenic region. Phylogenetic and distance analyses based on the rrs gene allocated Anaplasma sequences from sloths captured in Rondônia and Pará states in a single clade, which was closely related to the A. marginale , A. ovis, and A. capra clades. The sequences detected in southern anteaters from São Paulo were allocated in a clade closely related to sequences of Anaplasma spp. detected in Nasua nasua , Leopardus pardalis , and Cerdocyon thous in Brazil. These sequences were positioned close to A. odocoilei sequences. Genotype analysis corroborated previous findings and demonstrated the circulation of two distinct Anaplasma genotypes in animals from north and southeast Brazil. The first genotype was new. The second was previously detected in N. nasua in Mato Grosso do Sul state. The intergenic region analyses also demonstrated two distinct genotypes of Anaplasma . The sequences detected in Xenarthra from Pará and Rondônia states were closely related to those in A. marginale , A. ovis, and A. capra . Anaplasma spp. sequences detected in Xenarthra from São Paulo and were allocated close to those in A. phagocytophilum . The analyses based on the dsb gene grouped the Ehrlichia spp. sequences with sequences of E. canis (São Paulo, Mato Grosso do Sul, and Pará) and E. minasensis (Rondônia and Pará). The data indicate the occurrence of E....

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“…and Anaplasma spp. in wild carnivores (André, 2018;André et al, 2010André et al, , 2012, deer (Sacchi et al, 2012), wild rodents (Benevenute et al, 2017), opossums (Guimarães et al, 2019) and Xenarthra mammals (Calchi et al, 2020) indicates a great diversity of Anaplasmataceae agents circulating in wild mammals. On this pattern, screening wild animals for Anaplasmataceae agents, especially those that are closely…”

Section: Resultsmentioning

confidence: 99%

‘ Candidatus Mycoplasma haematonasua’ and tick‐borne pathogens in ring‐tailed coatis ( Nasua nasua Linnaeus, 1976) from the Iguaçu National Park, Paraná State, southern Brazil

Collere

Delai

Ferrari

et al. 2021

Transbound Emerg Dis

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The Iguaçu National Park (INP) is the largest remnant of Atlantic Forest in southern Brazil, representing an ecological continuum with Argentina. The INP harbours a diverse fauna, with ring-tailed coatis (Nasua nasua Linnaeus, 1976, Carnivora: Procyonidae) in close contact with tourists either begging and/or snatching food from visitors. A potentially novel haemotropic Mycoplasma sp. has been previously detected in the ring-tailed coatis from central-western and southern Brazil. Therefore, the aims of this study were to investigate the occurrence of haemotropic Mycoplasma sp. and tickborne pathogens in wild ring-tailed coatis from the INP, Foz do Iguaçu municipality, Paraná State, southern Brazil. Blood samples were collected from 18 wild ring-tailed coatis and evaluated by conventional PCR (cPCR) assays for haemotropic Mycoplasma spp. (16S and 23S rRNA), Theileria/Babesia spp. (18S rRNA) and Ehrlichia/Anaplasma spp. (16S rRNA, sodB, dsb and groEL). Eight out of 18 (44.44%; 95% CI: 24.56%-66.28%) animals were positive for haemotropic Mycoplasma spp. All ring-tailed coatis tested negative for Theileria/Babesia spp. and only one out of 18 (5.56%; 95% CI: 0.99%-25.76%) animals tested positive for Ehrlichia/Anaplasma spp. by the 16S rRNA cPCR. Unfortunately, multiple attempts to sequence the 16S rRNA gene of the Ehrlichia/Anaplasma-positive sample have failed. Phylogenetic and network analysis of the hemoplasma 16S and 23S rRNA gene fragments confirmed that animals were infected by a potentially novel haemotropic Mycoplasma sp. previously reported in ringtailed coatis from Brazil. The name 'Candidatus Mycoplasma haematonasua' is proposed for this novel organism.

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Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphis aurita) from Brazil

Cited by 14 publications

References 30 publications

‘Candidatus Mycoplasma Haemoalbiventris’ and Tick-Borne Pathogens in Black-Eared Opossum (Didelphis aurita) from Southeastern Brazil

‘Candidatus Mycoplasma Haemoalbiventris’ and Tick-Borne Pathogens in Black-Eared Opossum (Didelphis aurita) from Southeastern Brazil

Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

‘ Candidatus Mycoplasma haematonasua’ and tick‐borne pathogens in ring‐tailed coatis ( Nasua nasua Linnaeus, 1976) from the Iguaçu National Park, Paraná State, southern Brazil

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