Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

Beers, Joyce J B C van; Vanderlocht, Joris; Roozendaal, Caroline; Damoiseaux, Jan

doi:10.1007/978-1-4939-8949-2_4

Cited by 13 publications

(15 citation statements)

References 48 publications

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“…The remaining patients tested positive for PR3-ANCA. P-ANCA is found not only in patients with MPO-ANCA but also in patients with antibodies to lactoferrin, elastase, cathepsin G, lysozyme, and other antigens (33). Recently, antilactoferrin antibodies were detected in patients with EGPA (4/19, 21.1%) but not in patients with GPA or MPA.…”

Section: Prevalence Of Anca In Patients With Egpamentioning

confidence: 99%

International Consensus on Antineutrophil Cytoplasm Antibodies Testing in Eosinophilic Granulomatosis with Polyangiitis

Moiseev

Bossuyt

Arimura

et al. 2020

Am J Respir Crit Care Med

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Section: Prevalence Of Anca In Patients With Egpamentioning

confidence: 99%

International Consensus on Antineutrophil Cytoplasm Antibodies Testing in Eosinophilic Granulomatosis with Polyangiitis

Moiseev

Bossuyt

Arimura

et al. 2020

Am J Respir Crit Care Med

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“…ANCA might be commonly directed against proteinase 3 (PR3) and myeloperoxidase (MPO), although it is believed that autoantibodies towards different antigens (e.g., elastase, cathepsin G, lactoferrin, lysozyme) also elicit a positivity in indirect immunofluorescence (IIF), which can return cytoplasmic or perinuclear patterns [140][141][142][143].…”

Section: Laboratory Testing In Ibd: Autoimmunity Markersmentioning

confidence: 99%

Inflammation, Autoinflammation and Autoimmunity in Inflammatory Bowel Diseases

Padoan

Musso

Contran

et al. 2023

CIMB

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In this review, the role of innate and adaptive immunity in the pathogenesis of inflammatory bowel diseases (IBD) is reported. In IBD, an altered innate immunity is often found, with increased Th17 and decreased Treg cells infiltrating the intestinal mucosa. An associated increase in inflammatory cytokines, such as IL-1 and TNF-α, and a decrease in anti-inflammatory cytokines, such as IL-10, concur in favoring the persistent inflammation of the gut mucosa. Autoinflammation is highlighted with insights in the role of inflammasomes, which activation by exogenous or endogenous triggers might be favored by mutations of NOD and NLRP proteins. Autoimmunity mechanisms also take place in IBD pathogenesis and in this context of a persistent immune stimulation by bacterial antigens and antigens derived from intestinal cells degradation, the adaptive immune response takes place and results in antibodies and autoantibodies production, a frequent finding in these diseases. Inflammation, autoinflammation and autoimmunity concur in altering the mucus layer and enhancing intestinal permeability, which sustains the vicious cycle of further mucosal inflammation.

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“…The hand-out is primarily focused on commercially available assays for clinical purposes. For inhouse assays there exist detailed protocols (13,27), but they require a more extended validation, which is beyond the scope of the current paper. Important items for the laboratory evaluation are reproducibility (intra-and inter-assay variability), carry-over in analyzers, and linearity (vide supra).…”

Section: Implementation Of Anca Assays In Clinical Practicementioning

confidence: 99%

“…For diagnostic purposes, ANCA screening was originally performed by indirect immunofluorescence (IIF) assays on a substrate of ethanol-fixed neutrophils (12,13). Positive samples were to be analyzed for reactivity to proteinase 3 (PR3) and myeloperoxidase (MPO) (14).…”

Section: Introductionmentioning

confidence: 99%

ANCA Testing in Clinical Practice: From Implementation to Quality Control and Harmonization

Damoiseaux

2021

Front. Immunol.

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Analyses for the presence of anti-neutrophil cytoplasmic antibodies (ANCA) are important in the diagnostic work-up of patients with small vessel vasculitis. Since current immuno-assays are predominantly designed for diagnosis of patients with ANCA-associated vasculitis (AAV), implementation in routine clinical practice, internal and external quality control, and harmonization are focused on this particular use. However, ANCA testing may also be relevant for monitoring therapy efficacy and for predicting a clinical relapse in AAV patients, and even for diagnostic purposes in other clinical situations. In the current review, the topics of implementation, quality control, and standardization vs. harmonization are discussed while taking into account the different applications of the ANCA assays in the context of AAV.

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Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

Cited by 13 publications

References 48 publications

International Consensus on Antineutrophil Cytoplasm Antibodies Testing in Eosinophilic Granulomatosis with Polyangiitis

International Consensus on Antineutrophil Cytoplasm Antibodies Testing in Eosinophilic Granulomatosis with Polyangiitis

Inflammation, Autoinflammation and Autoimmunity in Inflammatory Bowel Diseases

ANCA Testing in Clinical Practice: From Implementation to Quality Control and Harmonization

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