Detection of antibiotic resistance, virulence gene determinants and biofilm formation in Aeromonas species isolated from cattle

Abstract

This study aimed to assess the antibiogram of Aeromonas strains recovered from cattle faeces and the potential pathogenic status of the isolates. The antibiogram of the Aeromonas isolates demonstrated total resistance to clindamycin oxacillin, trimethoprim, novobiocin and ticarcillin. However, Aeromonas strains were sensitive to cefotaxime, oxytetracycline and tobramycin. The Aeromonas strains from Lovedale and Fort Cox farms were found to possess some virulence genes. The percentage distribution was aer 71.4%, ast 35.7%, fla 60.7%, lip 35.7% and hlyA 25% for Lovedale farm and aer 63.1%, alt 10.5%, ast 55.2%, fla 78.9%, lip 21% and hlyA 35.9% for Fort Cox farm. Class 1 integron was present in 27% of Aeromonas isolates; the bla TEM gene was present in 34.8%, while the blaP1 class A β-lactamase gene was detected in 12.1% of the isolates. Approximately 86% of the isolates formed a biofilm on microtitre plates. The presence of multiple antibiotic resistance and virulence genes in Aeromonas isolates from cattle faeces reveals the pathogenic and infectious importance of these isolates and is of great significance to public health. The possession of a biofilm-forming capability by such isolates may lead to difficulty during the management of infection related to Aeromonas species.

N-Acylhomoserine Lactones (AHLs), QseB/C Gene Detection, Virulence Factors and Antibiotics Resistance of <i>Aeromonas hydrophila</i>

“…All the isolates from our study are strong biofilm producers. Igbinosa et al (2015) revealed that almost 86% of isolated Aeromonas hydrophila produce biofilm [33]. Our results are therefore in agreement with their findings.…”

Biofilm-Producing Ability and Antibiotic Resistance Pattern of Pathogenic Strains of Aeromonas hydrophila

“…Biofilm formation, a characteristic that can influence the pathogenicity of Aeromonas species, was detected in 71.4% of A. hydrophila isolates according to Guerra et al (34). Another study by Igbinosa et al showed that 86% of the isolates were biofilmproducers in the microtiter plate method (35). A similar conclusion was drawn by Ormanci and Yucel that reported biofilm formation by diverse Aeromonas species (food and environmental isolates) during 24 h (32).…”

Antibiotics modulate biofilm formation in fish pathogenic isolates of atypical Aeromonas salmonicida

“…Biofilm formation by Aeromonas spp., including A. salmonicida , has been reported previously (Chenia & Duma, 2017; Dias et al., 2018; Gavín et al., 2002; Hossain, Wickramanayake, Dahanayake, & Heo, 2020; Igbinosa, Igbinosa, & Okoh, 2015; Talagrand‐Reboul et al., 2017). In this present study, more than half (15/28) of the atypical A. salmonicida isolates from cleaner fish formed biofilm in vitro in the absence of antibiotics, which concurs with a previous study of A. salmonicida collected from the mussel Mytilus coruscus where 8/14 isolates formed biofilm (Hossain et al., 2020).…”