Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

Onodera, Takeshi; Miyahara, Kazuhisa; Iwakura, Munehiro; Hayashi, Kenshi; Miura, Norio; Matsumoto, Kiyoshi; Toko, Kiyoshi

doi:10.1541/ieejsmas.126.621

Cited by 8 publications

(4 citation statements)

References 7 publications

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“…16 Therefore, although the binding of HCDR3 to TNT was evaluated in aqueous phase in this study, molecular recognition might be also observed in the gas phase. Since saturating concentration of TNT is 7.5 ppb in the air, 36 further improvement in the dissociation constant would be necessary for TNT targeting.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Therefore, although the binding of HCDR3 to TNT was evaluated in aqueous phase in this study, molecular recognition might be also observed in the gas phase. Since saturating concentration of TNT is 7.5 ppb in the air, further improvement in the dissociation constant would be necessary for TNT targeting. The acquisition of various VOC recognizing peptide probes including TNT binding probe found in this study would provide us new sensor systems for environmental monitoring.…”

Section: Resultsmentioning

confidence: 99%

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Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody

et al. 2017

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Complementarity-determining regions (CDRs) are sites on the variable chains of antibodies responsible for binding to specific antigens. In this study, a short peptide probe for recognition of 2,4,6-trinitrotoluene (TNT), was identified by testing sequences derived from the CDRs of an anti-TNT monoclonal antibody. The major TNT-binding site in this antibody was identified in the heavy chain CDR3 by antigen docking simulation and confirmed by an immunoassay using a spot-synthesis based peptide array comprising amino acid sequences of six CDRs in the variable region. A peptide derived from heavy chain CDR3 (RGYSSFIYWF) bound to TNT with a dissociation constant of 1.3 μM measured by surface plasmon resonance. Substitution of selected amino acids with basic residues increased TNT binding while substitution with acidic amino acids decreased affinity, an isoleucine to arginine change showed the greatest improvement of 1.8-fold. The ability to create simple peptide binders of volatile organic compounds from sequence information provided by the immune system in the creation of an immune response will be beneficial for sensor developments in the future.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody

et al. 2017

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“…To use an SPR sensor system as an electronic dog nose at the secondary check of bomb detection after an initial check with a metal detector or another method, a vapor-sampling method was applied for the SPR sensor system. A preconcentrator, made up of a glass tube (internal diameter: 4 mm) filled with porous polymer beads as an adsorbent, was used in the sampling method for formation of a solution of nitroaromatic compounds [ 63 ]. The beads were Tenax-TA (60/80 mesh), which is commercially available and appropriate for concentrating volatile and semivolatile compounds.…”

Section: Sampling Methodsmentioning

confidence: 99%

Towards an Electronic Dog Nose: Surface Plasmon Resonance Immunosensor for Security and Safety

Onodera

Toko

2014

Sensors

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This review describes an “electronic dog nose” based on a surface plasmon resonance (SPR) sensor and an antigen–antibody interaction for security and safety. We have concentrated on developing appropriate sensor surfaces for the SPR sensor for practical use. The review covers different surface fabrications, which all include variations of a self-assembled monolayer containing oligo(ethylene glycol), dendrimer, and hydrophilic polymer. We have carried out detection of explosives using the sensor surfaces. For the SPR sensor to detect explosives, the vapor or particles of the target substances have to be dissolved in a liquid. Therefore, we also review the development of sampling processes for explosives, and a protocol for the measurement of explosives on the SPR sensor in the field. Additionally, sensing elements, which have the potential to be applied for the electronic dog nose, are described.

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“…We have proposed a method that uses a surface plasmon resonance (SPR) immunosensor to satisfy the above requirements [2]. Thus far, we have examined the detection of 2,4,6-trinitrotoluene and 2,4-dinitrotoluene [3].…”

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confidence: 99%

Preparation and Characteristics of Rat Anti-1,3,5-Trinitroperhydro-1,3,5-Triazine (RDX) Monoclonal Antibody and Detection of RDX Using Surface Plasmon Resonance Immunosensor

et al. 2013

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In this paper, we develop a method of detecting 1,3,5-trinitroperhydro-1,3,5-triazine [research department explosive (RDX)]. RDX is one of the main components of plastic explosives. Initially, we prepare an anti-RDX monoclonal antibody that specifically binds to RDX. The antibody is prepared from hybridoma cells that are prepared by the iliac lymph node method. The dissociation constant of the antibody against RDX is estimated to be 9.6 nM from the results of indirect competitive enzyme-linked immunosorbent assay. We fabricate a sensor chip on which RDX analogues are immobilized through a self-assembled monolayer with oligo(ethylene glycol) chains. High reliability and low false recognition rate are particularly required for sensors used for detecting explosives. Therefore, the nonspecific adsorption of the fabricated sensor chip is evaluated to confirm whether the sensor chip inhibits this nonspecific adsorption. The response characteristics of the prepared antibody against RDX are examined by indirect competitive assay. Under an incubation time of 0 min in the indirect competitive assay, a detection limit of 40 ppt is realized at a sample injection duration of 6 min.

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Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

Cited by 8 publications

References 7 publications

Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody

Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody

Towards an Electronic Dog Nose: Surface Plasmon Resonance Immunosensor for Security and Safety

Preparation and Characteristics of Rat Anti-1,3,5-Trinitroperhydro-1,3,5-Triazine (RDX) Monoclonal Antibody and Detection of RDX Using Surface Plasmon Resonance Immunosensor

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