Detection of biomarkers in body fluids using bioprobes based on aggregation-induced emission fluorogens

Abstract

<p>Body fluids (biofluids) are used as a means of examining various biomarkers of health and disease both as a means of rapid diagnosis and monitoring of chronic health conditions. Biomarker...</p>

Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

“…oligomer showed strong linear correlation ranging between 5 pg/mL-10 ng/mL (R 2 =0.997), where the LoD was calculated at 1.22 pg/mL. 161,166 Thus, the response of the biosensor correlates to an amyloidoligomer concentration range within the detection range found in physiological serum of Alzheimer's 𝛽 disease patients (1.35-12.5 ng/mL). 167 The preparation of 12 amyloid-1-42 MIP biosensors under identical conditions demonstrated a relative 𝛽 standard deviation (RSD) value of 7.7% for the detection of 1 ng/mL of amyloid-1-42 oligomer, 𝛽 indicating that the MIP could be reproduced satisfactorily.…”

Optical-Based Biosensors and Their Portable Healthcare Devices for Detecting and Monitoring Biomarkers in Body Fluids

“…This research provides a potential for applying AIE in tracking the presence of L-Lactate in saliva and urine samples. Meanwhile, Zhang et al [ 4 ] reviewed the constituents and clinical biomarkers in urine, saliva, and sweat, and the role of currently developed AIE bioprobes that can quantitatively detect disease-related biomarkers in these biofluids. The review also highlighted that several applications of AIE bioprobes, such as paper-based strips and portable devices, are currently under development, and have the potential to be realised simply by use with smartphone data capture and analysis, and data cloud storage.…”

“…For instance, human urine consists of urea, chloride, sodium and potassium salts, creatinine, and other substances; saliva contains sodium, calcium, phosphates, enzymes, mucins, and so on; blood and serum are comprised of glucose, copper, iron, white blood cells, red blood cells, albumin, cTnI, a prostate specific antigen, ascorbic acid, and so on [ 4 , 5 ]. Meanwhile, minerals, lactic acid, urea, electrolytes, nickel, lead, copper, iron, and zinc can be found in sweat [ 4 , 5 ]. Depending on the testing purpose, researchers can focus on collecting specific biomarkers through a desired body fluid.…”

Portable Colorimetric Device with Commercial Microplates for Quantitative Detection of Urine Biomarkers: Design, Development, and Clinical Evaluation

“…Another parameter for consideration when developing a chemical analysis device is the target analyte. As mentioned above, portable colorimetry devices can detect various chemical or biomolecules in solutions, which can be used as biomarkers, such as the presence of albumin in urine indicating kidney disease, a reduced citrate level in urine suggesting prostate cancer, the level of cardiac Troponin I (cTnI) in blood helping the diagnosis of acute myocardial infarction, or the absence of glutathione in saliva indicating the potential of head-neck cancer [ 5 , 18 , 19 , 20 , 21 ]. The detection target will influence the design choices, such as the sensitivity of the detecting sensor, the background light intensity, and the recognising reagent type.…”