The microscopic-blood image has been used to diagnose various diseases according to the morphological specifications of red and white blood cells. <span lang="EN-US">However, the manual analysis and procedures are not accurate due to the human error. Therefore, several studies conducted to find new techniques to perform this analysis using computer algorithms. The complexity of these algorithms led to thinking in simpler ways or to the hardware solutions. On the other hand, edge detection is a mathematical procedure that play an essential role in the field of medical image processing. It is considered as one of the foundations' processes for other procedures, such as the segmentation and the classification of the image. The Sobel filter is one of the conventional methods that is used to perform the edge detection process. It is based on finding the local contrast for the level of intensity of the image. This paper presents a proposed and a new method for detecting the edges of cells in the microscopic blood images using Sobel filter and its hardware implementation on the field programmable gate array (FPGA) chip. Three different techniques are proposed: MATLAB, OpenCV standard code, and FPGA customize code which give the best visual results, minimum timing results than the others.</span>