Detection of circulating antigen in serum of mice infected with Schistosoma japonicum by immunomagnetic bead ELISA based on IgY

Lei, Jiahui; Liu, Wenqi; Sun, Chengsong; Tang, Chaoliang; Li, Man-jun; Chen, Yuli; Lü, Yonglong

doi:10.1016/j.actatropica.2009.02.012

Cited by 23 publications

(23 citation statements)

References 38 publications

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“…In this respect, a novel immunomagnetic bead ELISA based on IgY (egg yolk immunoglobulin) has been developed for detection of circulating egg antigen in sera of (114) although it has yet to be assessed using human sera. Additional supportive clinical and laboratory findings for diagnosis of schistosomiasis include evidence of peripheral blood eosinophilia, anemia (iron deficiency anemia, anemia of chronic disease, or macrocytic anemia), hypoalbuminemia, elevated urea and creatinine levels, and hypergammaglobulinemia (165).…”

Section: Immunological Methodsmentioning

confidence: 99%

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

et al. 2010

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SUMMARY The potential impact of the Three Gorges Dam (TGD) on schistosomiasis transmission in China has invoked considerable global concern. The TGD will result in changes in the water level and silt deposition downstream, favoring the reproduction of Oncomelania snails. Combined with blockages of the Yangtze River's tributaries, these changes will increase the schistosomiasis transmission season within the marshlands along the middle and lower reaches of the Yangtze River. The changing schistosome transmission dynamics necessitate a comprehensive strategy to control schistosomiasis. This review discusses aspects of the epidemiology and transmission of Schistosoma japonicum in China and considers the pathology, clinical outcomes, diagnosis, treatment, immunobiology, and genetics of schistosomiasis japonica together with an overview of current progress in vaccine development, all of which will have an impact on future control efforts. The use of synchronous praziquantel (PZQ) chemotherapy for humans and domestic animals is only temporarily effective, as schistosome reinfection occurs rapidly. Drug delivery requires a substantial infrastructure to regularly cover all parts of an area of endemicity. This makes chemotherapy expensive and, as compliance is often low, a less than satisfactory control option. There is increasing disquiet about the possibility that PZQ-resistant schistosomes will develop. Consequently, as mathematical modeling predicts, vaccine strategies represent an essential component in the future control of schistosomiasis in China. With the inclusion of focal mollusciciding, improvements in sanitation, and health education into the control scenario, China's target of reducing the level of schistosome infection to less than 1% by 2015 may be achievable.

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Section: Immunological Methodsmentioning

confidence: 99%

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

et al. 2010

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“…13,14 Recently, a novel IgY-based immunomagnetic bead sandwich ELISA (IgY-IMB-ELISA) was established in our laboratory to detect circulating antigens in serum samples from mice with murine schistosomiasis japonica. 15 In this previous study, we produced polyclonal IgY from chickens immunized with S. japonicum soluble egg antigen (SEA), which showed a high specificity and a high concentration of detection (average = 69 mg per egg). The high-quality IgY was then coupled to commercial magnetic beads and used as a capture antibody in sandwich ELISA.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an IgY-Based Immunomagnetic Enzyme-Linked Immunosorbent Assay System for Detection of Circulating Schistosoma japonicum Antigen in Serum Samples from Patients in China

Lei

Su²,

Xu³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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We have developed a novel egg yolk antibody (IgY)–coated magnetic beads antigen-capture immunoassay for detection of a circulating antigen of Schistosoma japonicum in serum samples of patients in schistosomiasis-endemic areas of China. This IgY-based immunomagnetic bead enzyme-linked immunosorbent assay (IgY-IMB-ELISA) uses polyclonal IgY-coated magnetic beads as a capture antibody, and a monoclonal IgG as a detection antibody. The sensitivity of the magnetic immunoassay was 100% (40 of 40) in cases of acute infection and 91.5% (107 of 117) in chronic cases of schistosomiasis, and no positive reaction was found in 0 of 49 healthy persons. Cross-reactivity was 3.3% (1 of 33) with clonorchiasis and 0% (0 of 20) with paragonimiasis. There was a significant correlation between ELISA absorbance value and egg count (eggs per gram feces) and a correlation coefficient of 0.88 in a small sample of 14 patients. The results demonstrated that the IgY-IMB-ELISA is a sensitive and specific assay for detection of human schistosomiasis japonica.

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“…Additionally, the amount of IgY produced in egg yolk is threefold greater than the amount of IgG in the same volume of rabbit serum (Cova 2005), the production expense is less than IgG (Cova 2005), IgY presents a reduction in background colour of immunoenzymatic assays when compared to IgG (Schade et al 2005) and the extraction of IgY is simpler than IgG (can be made merely by egg yolk collection instead of animals bleeding) (Cova 2005). Because of these advantages, IgY has been successfully used in immunoenzymatic assays for the detection of Schistosoma japonicum (Lei et al 2009), foot-and-mouth disease virus (Veerasami et al 2008), sendai virus in porcines (Kvietkauskaite & Acaite 2004) and Escherichia coli (Sunwoo et al 2006).…”

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confidence: 99%

An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y

Silva

Vasconcelos

Kappel

et al. 2012

Mem. Inst. Oswaldo Cruz

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Detection of circulating antigen in serum of mice infected with Schistosoma japonicum by immunomagnetic bead ELISA based on IgY

Cited by 23 publications

References 38 publications

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

Evaluation of an IgY-Based Immunomagnetic Enzyme-Linked Immunosorbent Assay System for Detection of Circulating Schistosoma japonicum Antigen in Serum Samples from Patients in China

An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y

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