Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus

Honda, Ryoma; Nishifuji, Koji; Olivry, Thierry; White, Stephen D.; Momoi, Yasuyuki; Iwasaki, T.

doi:10.1016/j.rvsc.2004.03.009

Cited by 14 publications

(19 citation statements)

References 24 publications

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“…Remarkably, immunoabsorption of this patient's serum with recombinant canine desmoglein-1 completely abolished previously positive indirect immunofluorescence on living MCA-B1 canine keratinocytes, a cell line that expresses both desmogleins 1 and 3. 4 These results suggest that this dog's circulating autoantibodies were directed against the extracellular segment of desmoglein-1.…”

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confidence: 84%

Canine Hyperplastic Intraepidermal Pustular and Suprabasal Acantholytic Dermatosis with Features of Human Pemphigus Vegetans

et al. 2007

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Abstract. Pemphigus vegetans is a rare autoimmune blistering acantholytic dermatosis of humans that combines unusually hyperplastic and verrucous pustular skin lesions and mucosal erosions. We report herein the clinical, histopathologic, and immunologic findings in a dog whose lesions resembled, but were not identical to, those of human pemphigus vegetans. A 4-year-old male Greater Swiss Mountain Dog presented with multifocal cutaneous verrucous and crusted papules and pustules, as well as skin and mucosal erosions and ulcers. Microscopic lesions consisted of exophytic papillated epidermal hyperplasia, superficial and deep intraepidermal acantholytic neutrophilic and eosinophilic pustules, and suprabasal epidermal clefts leaving rounded basal keratinocytes at the bottom of the vesicles. Direct and indirect immunofluorescence revealed antikeratinocyte IgG autoantibodies. Immunoprecipitation immunoblotting and immunoabsorption experiments with recombinant canine desmogleins confirmed that autoantibodies recognized desmoglein-1. In this dog, clinical and histopathologic features resembled those of human pemphigus vegetans, while circulating autoantibodies against canine desmoglein-1 were solely identified. This antigen target is different from that of the human disease in which antidesmoglein-3 autoantibodies are detected most commonly.

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confidence: 84%

Canine Hyperplastic Intraepidermal Pustular and Suprabasal Acantholytic Dermatosis with Features of Human Pemphigus Vegetans

et al. 2007

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“…2) [5,7]. Fluorescent deposition on the cell surface of keratinocytes could be observed when both substrates were used, but maximum IIF titer on bovine esophagus (1:10240) was considerably higher than that on living MCA-B1 cells (1:80).…”

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confidence: 96%

“…However, the sensitivity of IIF for diagnosis of canine PF could be improved when cryosectioned bovine esophagus was used as substrate, although a few dogs with non-pemphigus dermatoses showed false-positive immunoreactivity [5,7]. In addition, despite of its low sensitivity, living keratinocyte staining using a canine keratinocyte cell line, MCA-B1, has provided a highly reliable serological diagnostic tool to detect serum autoantibodies in canine PF with its high specificity [5]. These techniques have the potential to be used for monitoring the disease activity of canine PF, as similarly reported in human patients [6].…”

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confidence: 99%

A Canine Pemphigus Foliaceus Case Showing Parallel Relationship of Disease Activity and Titer of Serum Anti-keratinocyte Cell Surface Antibodies

Nishifuji

Yoshida-Yamakita²,

Iwasaki

2005

The Journal of Veterinary Medical Science

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ABSTRACT. A seven-year-old, spayed female mongrel dog was diagnosed as pemphigus foliaceus (PF) by clinical, histopathological and immunopathological observations. Serum antibodies against the cell surface of keratinocytes in the dog were detected by indirect immunofluorescence (IIF) using cryosectioned bovine esophagus as well as living cultured-canine keratinocytes as the substrates. When we compared the titers of IIF on bovine esophagus with its disease activity, the IIF titers reflected the disease activity throughout the time course. Our findings will suggest that sequential titration of serum antibodies by IIF will be useful for monitoring the serological disease activity in canine PF. KEY WORDS: canine, indirect immunofluorescence, pemphigus.J. Vet. Med. Sci. 67(9): 943-945, 2005

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“…Circulating autoantibodies against keratinocyte cell surfaces were recognized in dogs with PF, as determined by indirect immunofluorescence (IIF) examination on cryosections of normal epithelia or cultured canine keratinocytes [6,9]. Recent paper described that injection of serum IgG from three dogs with PF to neonatal mice caused acantholytic blisters at the superficial layer of epidermis [12].…”

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confidence: 99%

Neutrophils Contact to Plasma Membrane of Keratinocytes Including Desmosomal Structures in Canine Pemphigus Foliaceus

Yabuzoe

Nishifuji

Sekiguchi

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Pemphigus foliaceus (PF) is an autoimmune blistering skin disease that affects certain mammals including dogs. In canine PF, neutrophils are infiltrated intensely into pustular lesions including acantholytic cells, although neutrophilic infiltration is not characterized in human PF. The roles of the neutrophils in the cutaneous lesions of canine PF have not yet been understood. The purpose of this study was to characterize the ultrastructural features underlying the acantholysis with pustule formation in canine PF. Four dogs diagnosed as PF on the basis of clinical signs, histopathological findings, and direct and indirect immunofluorescence examinations were performed. Electron microscopy revealed that the acantholytic cells were adjacent to multiple neutrophils in the pustules. At the contact points between neutrophils and acantholytic keratinocytes, half-desmosomes of acantholytic keratinocytes with intact attachment plaques were observed within invaginations of neutrophils. Furthermore, on the surface of acantholytic cells in the pustules, neutrophil granules seemed to be secreted to the surface of acantholytic cells and to degenerate the half-desmosome structures. Neutrophils were also observed within the epidermis adjacent to the pustule. At the intercellular gap between two dissociated keratinocytes, neutrophils inserted its pseudopodia into the gap between the two half-desmosomes of keratinocytes. These findings taken together suggested that, at least in the areas where we analyzed ultrastructurally, neutrophils contact desmosomal structures and seem to play some parts in separation of keratinocytes and degeneration of split-desmosomes in pustules of dogs with PF.

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Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus

Cited by 14 publications

References 24 publications

Canine Hyperplastic Intraepidermal Pustular and Suprabasal Acantholytic Dermatosis with Features of Human Pemphigus Vegetans

Canine Hyperplastic Intraepidermal Pustular and Suprabasal Acantholytic Dermatosis with Features of Human Pemphigus Vegetans

A Canine Pemphigus Foliaceus Case Showing Parallel Relationship of Disease Activity and Titer of Serum Anti-keratinocyte Cell Surface Antibodies

Neutrophils Contact to Plasma Membrane of Keratinocytes Including Desmosomal Structures in Canine Pemphigus Foliaceus

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