Detection of circulating tumor cells in prostate cancer based on carboxylated graphene oxide modified light addressable potentiometric sensor

Gu, Yinmin; Cheng, Ju; Li, Yanjun; Shang, Zhiqun; Wu, Yuchen; Jia, Yunfang; Niu, Yuanjie

doi:10.1016/j.bios.2014.10.070

Cited by 64 publications

(34 citation statements)

References 32 publications

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“…Similarly, Ivanov et al (79) described a chip-based method using nanostructured microelectrodes and electrochemical readout, which confirmed the identity of isolated CTCs and successfully interrogated them for specific biomarkers. Recently, a light addressable potentiometric sensor was exploited in the label-free detection of CTCs in PCa, which may be a potential platform for CTC detection and may provide a powerful tool for downstream analysis (80). …”

Section: Enrichment and Detection Strategies For Ctcmentioning

confidence: 99%

Circulating tumor cells in prostate cancer: Precision diagnosis and therapy

et al. 2017

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The primary cause of tumor-associated mortality in prostate cancer (PCa) remains distant metastasis. The dissemination of tumor cells from the primary tumor to distant sites through the bloodstream cannot be detected early by standard imaging methods. Circulating tumor cells (CTCs) represent an effective prognostic and predictive biomarker, which are able to monitor efficacy of adjuvant therapies, detect early development of metastases, and finally, assess therapeutic responses of advanced disease earlier than traditional diagnostic methods. In addition, since repeated tissue biopsies are invasive, costly and not always feasible, the assessment of tumor characteristics on CTCs, by a peripheral blood sample as a liquid biopsy, represents an attractive opportunity. The implementation of molecular and genomic characterization of CTCs may contribute to improve the treatment selection and thus, to move toward more precise diagnosis and therapy in PCa. The present study summarizes the current advances in CTC enrichment and detection strategies and reviews how CTCs may contribute to significant insights in the metastatic process, as well as how they may be utilized in clinical application in PCa. Although it is proposed that CTCs may offer insights into the prognosis and management of PCa, there are a number of challenges in the study of circulating tumor cells, and their clinical utility remains under investigation.

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Section: Enrichment and Detection Strategies For Ctcmentioning

confidence: 99%

Circulating tumor cells in prostate cancer: Precision diagnosis and therapy

et al. 2017

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“…Other unique alternatives to IF are emerging for CTC detection, including the use of surface plasmon resonance, 48 surface enhanced raman spectroscopy, 49 ultrasound and photoacoustics, 50 electrochemical aptasensors, 51 silicon nano-wire-array detection of magnetic upconversion nanoparticles, 52 light addressable potentiometric sensors, 53 impedance-based detection, 54 and PCR-based methods. 55,56 However, VDC remains a top candidate as a truly label-free enrichment and enumeration platform, supported with preliminary tests using clinical samples.…”

Section: Resultsmentioning

confidence: 99%

Biophysical isolation and identification of circulating tumor cells

Che

Garon

et al. 2017

Lab Chip

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Isolation and enumeration of circulating tumor cells (CTCs) from blood is important for determining patient prognosis and monitoring treatment. Methods based on affinity to cell surface markers have been applied to both purify (via immunoseparation) and identify (via immunofluorescence) CTCs. However, variability of cell biomarker expression associated with tumor heterogeneity and evolution and cross-reactivity of antibody probes have long complicated CTC enrichment and immunostaining. Here we report a truly label-free high-throughput microfluidic approach to isolate, enumerate, and characterize the biophysical properties of CTCs on an integrated microfluidic device. Vortex-mediated Deformability Cytometry (VDC) consists of an initial vortex region which enriches for large CTCs, followed by release into a downstream hydrodynamic stretching region which deforms the cells. Visualization and quantification of cell deformation with a high-speed camera revealed populations of large (>15 μm diameter) and deformable (aspect ratio >1.2) CTCs from 16 stage IV lung cancer samples, that are clearly distinguished by increased deformability compared to contaminating blood cells and rare large cells isolated from healthy patients. VDC technology demonstrated a comparable positive detection rate of putative CTCs above healthy baseline (93.8%) with respect to standard immunofluorescence (71.4%). Automation allows full enumeration of CTCs from a 10mL vial of blood within <1 hr after sample acquisition, compared with 4+ hours with standard approaches. Moreover, cells are released into any collection vessel for further downstream analysis. VDC shows potential for accurate CTC enumeration without labels and confirms the unique highly deformable biophysical properties of large CTCs circulating in blood.

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“…Different types of nanostructured substrates have been reported for CTC enrichment, including nanoarray [65-71], nanofiber [72, 73], nanosheet [74, 75], deposited NP substrates [76-78] roughened surface [79, 80], and nanoporous substrates [81, 82]. The integrated NanoVelcro device, which has been developed by Tseng and co-authors through a combination of anti-EpCAM coated vertically oriented silicon nanopillars with vastly enhanced CTC-capture affinity and an overlaid microfluidic chaotic mixing chip capable of promoting cell-substrate contact frequency, is very promising [66] ( Figure 3B ).…”

Section: Nanostructured Substratesmentioning

confidence: 99%

Nanotechnology for Enrichment and Detection of Circulating Tumor Cells

Bhana

Wang

Huang

2015

Nanomedicine (Lond.)

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Circulating tumor cells (CTCs) are a hallmark of invasive behavior of cancer, responsible for the development of metastasis. Their detection and analysis have significant impacts in cancer biology and clinical practice. However, CTCs are rare events and contain heterogeneous subpopulations, requiring highly sensitive and specific techniques to identify and capture CTCs with high efficiency. Nanotechnology shows strong promises for CTC enrichment and detection owning to the unique structural and functional properties of nanoscale materials. In this review, we discuss the CTC enrichment and detection technologies based on a variety of functional nanosystems and nanostructured substrates, with the goal to highlight the role of nanotechnology in the advancement of basic and clinical CTC research.

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Detection of circulating tumor cells in prostate cancer based on carboxylated graphene oxide modified light addressable potentiometric sensor

Cited by 64 publications

References 32 publications

Circulating tumor cells in prostate cancer: Precision diagnosis and therapy

Circulating tumor cells in prostate cancer: Precision diagnosis and therapy

Biophysical isolation and identification of circulating tumor cells

Nanotechnology for Enrichment and Detection of Circulating Tumor Cells

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