Detection of concanavalin A based on attenuated fluorescence resonance energy transfer between quantum dots and mannose-stabilized gold nanoparticles

Lim, Kyung Rae; Ahn, Kwang Soo; Lee, Won Yong

doi:10.1039/c2ay26128f

Cited by 22 publications

(13 citation statements)

References 26 publications

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“…In Tris buffer, K sv was determined to be 6.38 × 10 7 M −1 (F 0 /F = 1.037 + 0.054 [AuNPs], r 2 = 0.999). This constant is quite similar to that obtained with mannose-stabilized AuNPs in our previous report [29]. This experimental result clearly indicates that the fluorescence emission of CdTe QDs is quenched by the galactose-stabilized AuNPs.…”

Section: Fret Between Qds and Aunpssupporting

confidence: 89%

“…In our previous work, we have shown that the hydrogen bonding between the amine groups of QDs surfaces and the hydroxyl groups of the α-mannose-stabilized on the AuNPs leads to the formation of AuNPs-QDs assembly [29]. In the present work, the identical hydrogen bonding formation was observed with β-galactose-stabilized AuNPs.…”

Section: Fret Between Qds and Aunpssupporting

confidence: 72%

“…4, the absorption spectrum of galactose-stabilized AuNPs showed maximum wavelength at around 520 nm, which was identical with that of mannose-stabilized AuNPs [29] because the absorption spectrum is attributed to the surface plasmon absorption band of AuNPs cores and is not dependent upon the capping glycoside on AuNPs.…”

Section: Fret Between Qds and Aunpsmentioning

confidence: 58%

“…We have previously reported that specially synthesized monosaccharide α-mannose derivative self-assembled on AuNPs has strong affinity toward mannose binding protein (lectin) such as concanavalin A (Con A) and these mannose-stabilized AuNPs can be used for the determination of Con A as signal amplifiers in quartz crystal microbalance (QCM) [27] and also in stripping analysis [28]. In addition, it is reported that FRET can be obtained by combining the mannose-stabilized AuNPs with quantum dots [29]. On addition of the target Con A leads to the recognition-induced inhibition of FRET and thus fluorescence recovery.…”

Section: Introductionmentioning

confidence: 98%

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Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

Ahn

Lim

Jeong

et al. 2016

Microchemical Journal

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Section: Fret Between Qds and Aunpssupporting

confidence: 89%

Section: Fret Between Qds and Aunpssupporting

confidence: 72%

Section: Fret Between Qds and Aunpsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 98%

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Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

Ahn

Lim

Jeong

et al. 2016

Microchemical Journal

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“…[22][23][24][25][26][27] It is well known that concanavalin A is a legume lectin from Jack beans containing four identical binding sites. Each monomer subunit of Con A is approximately 4 Â 4 Â 4 nm with a single small binding site capable of binding to a-D-glucopyranose with unmodified hydroxy groups at positions C3, C4 and C6.…”

Section: Introductionmentioning

confidence: 99%

A biosensing platform for sensitive detection of concanavalin A based on fluorescence resonance energy transfer from CdTe quantum dots to graphene oxide

Shi

Cai

2015

New J. Chem.

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In this paper, we constructed a novel fluorescence resonance energy transfer (FRET) system between CdTe quantum dots (QDs) and graphene oxide (GO) for sensitive detection of concanavalin A (Con A). In this system, CdTe QDs as an energy donor and GO as an energy acceptor were both covalently functionalized by glucosamine via an amide linkage. The specific combination of Con A with glucosamine could bring CdTe QDs and GO into appropriate proximity and therefore induce efficient energy transfer. The concentration of Con A caused modulation of the energy transfer efficiency between GO and QDs, thus enabling Con A detection. Under the optimized experimental conditions, the decrease of fluorescence intensity ratio I/I 0 (I 0 and I are the fluorescence intensity of the sensing system in the absence and presence of Con A, respectively) is proportional to the concentration of Con A in the range of 9.8-196.1 nM. This method has high sensitivity with the detection limit as low as 3.3 nM. The results suggested that the graphene FRET platform could be used for sensitive and selective sensing of Con A and could be applied for protein-carbohydrate interaction studies.

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Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Garcı́a

Mosquera

Plou

et al. 2018

Advanced Optical Materials

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regulation of a variety of physiological and pathological processes, such as proliferation and adhesion, immune response, and cell differentiation. [8] The biological roles of carbohydrates as signaling effectors and recognition markers are associated to specific molecular recognition events in which proteins [9] or other carbohydrates [10] are involved. However, individual carbohydrate-based molecular interactions have been shown to be generally weak and, therefore, biomolecules usually present clusters of carbohydrates that interact cooperatively with their natural ligands (multivalent effect), thereby increasing the binding strength. [11] Interestingly, the multivalent carbohydrate presentation can be mimicked by man-made multivalent systems, which involve multiple synthetic oligosaccharides, [12][13][14][15][16][17] and have been used to address basic studies, but also as probe molecules and drugs. Mammalian cells are covered by a dense array of carbohydrates known as glycocalyx. The glycocalyx composition depends on the specific cellular state, and therefore it can provide information about diseases. For example, an increase of α-2,6-sialylation, fucosylation, and/or other tumor-associated carbohydrate antigens on the cellular membrane is considered as a biomarker of cancer progression, with diagnostic value. [18,19] In addition, oligosaccharides can be used as disease biomarkers for targeted therapy, and therefore the analysis and characterization of the composition of cellular glycans provide information of relevance to the diagnosis of several diseases. [20] Different strategies can be used to label glycans, including the use of covalent recognizing ligands (e.g., boronic acid), using specific carbohydrate-binding proteins, and via metabolic labeling.Plasmonics is based on the excitation of surface plasmons, that is, coherent oscillations of electrons induced by an electromagnetic radiation at metal-dielectric interfaces. [21] When the dimensions of plasmonic nanostructures are smaller than the wavelength of the incident light, the interaction between the electromagnetic field and surface charges results in nonpropagating oscillations denoted as localized surface plasmon resonances (LSPR). [22] LSPR frequencies in metal nanoparticles depend on their size, shape, organization, interparticle distance, and dielectric environment, ranging from the visible through the near-infrared (NIR). [23,24] A large volume of research has been devoted to the development of reliable methods for the preparation of plasmonic nanostructures with well-defined shape, size, and/or interparticle spacing, as basic components of various analytical applications. [25,26] Another important element, the surface chemistry of plasmonic The incorporation of nanomaterials in glycoscience research enables the design of highly selective and sensitive bioanalytical devices for the detection of disease-associated biomarkers. In particular, localized surface plasmon resonance (LSPR) and surface enhanced Raman scattering (SERS) spectroscopies ar...

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Detection of concanavalin A based on attenuated fluorescence resonance energy transfer between quantum dots and mannose-stabilized gold nanoparticles

Cited by 22 publications

References 26 publications

Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

A biosensing platform for sensitive detection of concanavalin A based on fluorescence resonance energy transfer from CdTe quantum dots to graphene oxide

Plasmonic Detection of Carbohydrate‐Mediated Biological Events

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