Detection of different variants of Ostreid Herpesvirus 1 in the Pacific oyster, Crassostrea gigas between 2008 and 2010

Martenot, Claire; Oden, Elise; Travaillé, Emmanuelle; Malas, J.P.; Houssin, Maryline

doi:10.1016/j.virusres.2011.04.012

Cited by 106 publications

(72 citation statements)

References 24 publications

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“…Segarra et al (2014c) compared the susceptibility of bi-parental C. gigas families to OsHV-1 and confirmed that susceptibility to OsHV-1 infection had a significant genetic component. Viral DNA was detected earlier and the overall amount of viral DNA was higher for a low surviving oyster family compared with a high surviving family (Segarra et al, 2014c ] for mortality to occur (Oden et al, 2011). Similar observations are reported for abalone with a proportion of the population testing PCRpositive for AbHV (low amounts of viral DNA), but displaying no clinical signs of disease Dang et al, 2013).…”

Section: Genetics and Physiologysupporting

confidence: 66%

“…The virus has been purified from naturally infected Crassostrea gigas larvae (Le Deuff & Renault, 1999) and its genome entirely sequenced (Davison et al, 2005). Several genotypes of OsHV-1 have been detected by conventional PCR, targeting a specific area of the genome (Arzul et al, 2001b;Martenot et al, 2011;Segarra et al, 2010). The reference (OsHV-1 ref) and variant (OsHV-1 var.)…”

Section: Introductionmentioning

confidence: 99%

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Antiviral immunity in marine molluscs

Green

Raftos

Speck

et al. 2015

Journal of General Virology

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Marine molluscs, like all living organisms, are constantly exposed to viruses and have evolved efficient antiviral defences. We review here recent developments in molluscan antiviral immunity against viruses belonging to the order Herpesvirales. Emerging results suggest an interferon-like response and autophagy are involved in the antiviral defence of bivalves to viral infection. Multifunctional plasma proteins from gastropods and bivalves have been identified to have broadspectrum antiviral activity against mammalian viruses. The antiviral defences present in molluscs can be enhanced by genetic selection, as shown by the presence of oyster strains specifically resistant to ostreid herpesvirus type 1. Whether varying amounts or different isoforms of these antiviral plasma proteins contributes to genetic resistance is worthy of further research. Other evolutionarily conserved antiviral mechanisms, such as RNA interference and apoptosis, still need further characterization.

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Section: Genetics and Physiologysupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Antiviral immunity in marine molluscs

Green

Raftos

Speck

et al. 2015

Journal of General Virology

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“…Over recent years, the production of oysters has declined with abnormal mortality events of Crassostrea gigas adults reported in most French oyster production areas, generally linked with oyster herpes virus (Martenot et al 2011). It is now thought that the bacterial pathogens Vibrio splendidus and V. aestuarianus may play a more significant role in the mortality events, with interactions between pathogens and the development of some resistance by previously infected stocks playing a role in the severity of losses (Vezzulli et al 2015;Petton et al 2015).…”

Section: Shellfishmentioning

confidence: 99%

An assessment of the economic contribution of EU aquaculture production and the influence of policies for its sustainable development

Bostock

Lane

Hough³

et al. 2016

Aquacult Int

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This review paper examines the structure of the EU aquaculture sector, the contribution it makes to the EU economy and the policy environment for past and future development. The primary analysis uses statistical data from the Food and Agriculture Organization of the United Nations which has been re-categorized according to species groups established by the European Aquaculture Technology and Innovation Platform (EATiP) and by culture system type using expert knowledge. Additional data sources for the analysis include the European Market Observatory for Fisheries and Aquaculture Products (EUMOFA) and the European Commission Scientific, Technical and Economic Committee for Fisheries. EU aquaculture production was 1.34 million tonnes in 2012 with a first sale value of €4.76 billion. Shellfish comprised 45 % by volume and 28 % by value; marine fish 30 % by volume and 53 % by value; and freshwater fish 25 % by volume and 19 % by value. The total production volume has actually fallen slightly from 1.4 million tonnes in 2000, whilst the value has increased significantly from 2.79 billion in 2000, mainly due to a growth in Atlantic salmon production. Five countries accounted for around 78 % of the direct output value of EU aquaculture in 2012, the UK, France, Greece, Italy and Spain. Around 50 % of the direct output value was generated using marine cage systems (28 % by volume), whilst less than 3 % of value was generated in recirculated aquaculture systems (\1.5 % by volume). Around 5 % of value was contributed by extensive to semi-intensive inland and coastal pond systems. STECF (2014) estimates there are between 14,000 and 15,000 aquaculture enterprises in the EU employing around 80,000 people, approximately 40,000 full-time equivalent (FTE). The greatest number of jobs (FTE) is provided by the freshwater pond and suspended shellfish sectors due to much lower productivity figures. This could be seen as a social benefit in rural and coastal regions, but corresponding low wages could also discourage young entrants to the industry and lead to dependency on migrant workers. Where efficiencies can be improved through capital investment there is likely to be significant scope for consolidation of ownership as can be observed in the marine fish sector. The output from aquaculture has to find a place within the wider fish and seafood market where volumes are generally inversely related to price. The potential growth of the sector is therefore constrained both in relation to the overall market and with respect to competition from substitute products. These include product from EU capture fisheries as well as imports from third countries (sourced from aquaculture and capture fisheries). Whilst interactions between individual products can be hard to demonstrate, any increase in production costs is likely to lead to lower output volumes, whilst improvements in production efficiency can lead to increased output volumes. With around 60 % of EU fish and seafood supply obtained through imports, and little prospect of incre...

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“…Since 2008, massive mortality associated with ostreid herpesvirus 1 (OsHV-1) outbreaks has been reported in Europe (mainly in France) among Crassostrea gigas spat and juveniles (9)(10)(11)(12)(13)(14)(15). Later, the virus was associated with major mortalities in Pacific oysters from Australia and Asia (16)(17)(18).…”

mentioning

confidence: 99%

Antiviral Activity of Myticin C Peptide from Mussel: an Ancient Defense against Herpesviruses

Novoa

Romero

Álvarez

et al. 2016

J Virol

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Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering activity, interaction with putative pathogens, including viruses, is very high, suggesting that they should have mechanisms to address these infections. In this study, we confirmed that constitutively expressed molecules in naive mussels confer resistance in oysters to ostreid herpesvirus 1 (OsHV-1) when oyster hemocytes are incubated with mussel hemolymph. Using a proteomic approach, myticin C peptides were identified in both mussel hemolymph and hemocytes. Myticins, antimicrobial peptides that have been previously characterized, were constitutively expressed in a fraction of mussel hemocytes and showed antiviral activity against OsHV-1, suggesting that these molecules could be responsible for the antiviral activity of mussel hemolymph. For the first time, a molecule from a bivalve has shown antiviral activity against a virus affecting mollusks. Moreover, myticin C peptides showed antiviral activity against human herpes simplex viruses 1 (HSV-1) and 2 (HSV-2). In summary, our work sheds light on the invertebrate antiviral immune response with the identification of a molecule with potential biotechnological applications. IMPORTANCESeveral bioactive molecules that have potential pharmaceutical or industrial applications have been identified and isolated from marine invertebrates. Myticin C, an antimicrobial peptide from the Mediterranean mussel (Mytilus galloprovincialis) that was identified by proteomic techniques in both mussel hemolymph and hemocytes, showed potential as an antiviral agent against ostreid herpesvirus 1 (OsHV-1), which represents a major threat to the oyster-farming sector. Both hemolymph from mussels and a myticin C peptide inhibited OsHV-1 replication in oyster hemocytes. Additionally, a modified peptide derived from myticin C or the nanoencapsulated normal peptide also showed antiviral activity against the human herpesviruses HSV-1 and HSV-2. Therefore, myticin C is an example of the biotechnological and therapeutic potential of mollusks.

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Detection of different variants of Ostreid Herpesvirus 1 in the Pacific oyster, Crassostrea gigas between 2008 and 2010

Cited by 106 publications

References 24 publications

Antiviral immunity in marine molluscs

Antiviral immunity in marine molluscs

An assessment of the economic contribution of EU aquaculture production and the influence of policies for its sustainable development

Antiviral Activity of Myticin C Peptide from Mussel: an Ancient Defense against Herpesviruses

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