Detection of DNA oligonucleotides on nanowire array electrodes using chronocoulometry

Andreu, Aja; Merkert, Jon W.; Lecaros, Luis A.; Broglin, Brandy L.; Brazell, J. Tres; El‐Kouedi, Mahnaz

doi:10.1016/j.snb.2005.07.072

Cited by 38 publications

(22 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Perhaps even more exciting, however, are novel developments in PCR-free DNA detection. Rearchers have explored various possibilities, including nanowire circuits (Andreu et al 2006;Hahm and Lieber 2004;Li et al 2004), ''biosensing magnetic beads'' (Dubus et al 2006), and optical resonance of oligonucleotide coated silica spheres. All of these approaches avoid the necessity of DNA amplification, thus removing a significant source of potential bias (see text).…”

Section: Discussionmentioning

confidence: 99%

DNA-based methods for monitoring invasive species: a review and prospectus

2007

View full text Add to dashboard Cite

The recent explosion of interest in DNA-based tools for species identification has prompted widespread speculation on the future availability of inexpensive, rapid, and accurate means of identifying specimens and assessing biodiversity. One applied field that may benefit dramatically from the development of such technologies is the detection, identification, and monitoring of invasive species. Recent studies have demonstrated the feasibility of DNA-based tools for such important tasks as confirmation of specimen identity and targeted screening for known or anticipated invaders. However, significant technological hurdles must be overcome before more ambitious applications, including estimation of propagule pressure and comprehensive surveys of complex environmental samples, are to be realized. Here we review existing methods, examine the technical difficulties associated with development of more sophisticated tools, and consider the potential utility of these DNA-based technologies for various applications relevant to invasive species monitoring.

show abstract

Section: Discussionmentioning

confidence: 99%

DNA-based methods for monitoring invasive species: a review and prospectus

2007

View full text Add to dashboard Cite

show abstract

“…However, other materials such as gold (Andreu et al, 2006;Fang and Kelley, 2009), gallium nitride (Chen et al, 2011), carbon nanotubes (Sorgenfrei et al, 2011;Star et al, 2006), graphene oxide (Stine et al, 2010) and indium oxide (Tang et al, 2005) have been used. It is also possible to exploit NWs for oligonucleotide sensing in ways other than the FET-based approach that has been described.…”

Section: Nanowiresmentioning

confidence: 99%

“…It is also possible to exploit NWs for oligonucleotide sensing in ways other than the FET-based approach that has been described. Andreu et al (2006) and Fang and Kelley (2009) have both used coulometry to measure the change in charge caused by analyte nucleotides hybridising to the surface of NWs; however the FETbased approach is by far the most common. Zhang et al (2008Zhang et al ( , 2009Zhang et al ( , 2010aZhang et al ( , 2010b demonstrated SiNWbased oligonucleotide detection with LODs ranging from 100 fM to 1 fM, using nucleic acid analogue capture probes.…”

Section: Nanowiresmentioning

confidence: 99%

Micro- and nano-structure based oligonucleotide sensors

Ferrier

Shaver

Hands

2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

This paper presents a review of micro- and nano-structure based oligonucleotide detection and quantification techniques. The characteristics of such devices make them very attractive for Point-of-Care or On-Site-Testing biosensing applications. Their small scale means that they can be robust and portable, their compatibility with modern CMOS electronics means that they can easily be incorporated into hand-held devices and their suitability for mass production means that, out of the different approaches to oligonucleotide detection, they are the most suitable for commercialisation. This review discusses the advantages of micro- and nano-structure based sensors and covers the various oligonucleotide detection techniques that have been developed to date. These include: Bulk Acoustic Wave and Surface Acoustic Wave devices, micro- and nano-cantilever sensors, gene Field Effect Transistors, and nanowire and nanopore based sensors. Oligonucleotide immobilisation techniques are also discussed.

show abstract

“…The electrochemical methods involving nanofibers and nanotubes also exhibited concentration sensitivity of about 30 fM (Yang et al 2009). Nanowires (Andreu et al 2006;Gao et al 2007;Hahm and Lieber 2003;Zhang et al 2010;Zheng et al 2005) and nanotubes (Chang et al 2007;Wang et al 2003b) exhibited concentration sensitivity ranging from 100 fM to 1 fM. Microcantilevers coupled with nano-metal particles exhibited 0.01 nM concentration sensitivity (Su et al 2003).…”

Section: Introductionmentioning

confidence: 96%

Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification

Han

Soylu

Kirimli

et al. 2019

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Current genetic detection methods require gene isolation, gene amplification and detection with a fluorescent-tagged probe. They typically require sophisticated equipment and expensive fluorescent probes, rendering them not widely available for rapid acute infection diagnoses at the point of care to ensure timely treatment of the diseases. Here we report a rapid genetic detection method that can detect the bacterial gene directly from patient stools using a piezoelectric plate sensor (PEPS) in conjunction with a continuous flow system with two temperature zones. With stools spiked with sodium dodecyl sulfate (SDS) in situ bacteria lysing and DNA denaturation occurred in the high-temperature zone whereas in situ specific detection of the denatured DNA by the PEPS occurred in the lower-temperature zone. The outcome was a rapid genetic detection method that directly detected bacterial genes from stool in <40 min without the need of gene *

show abstract

Detection of DNA oligonucleotides on nanowire array electrodes using chronocoulometry

Cited by 38 publications

References 17 publications

DNA-based methods for monitoring invasive species: a review and prospectus

DNA-based methods for monitoring invasive species: a review and prospectus

Micro- and nano-structure based oligonucleotide sensors

Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification

Contact Info

Product

Resources

About