Detection of EBV and HPV in nasopharyngeal carcinoma by in situ hybridization

Mirzamani, Neda; Salehian, Pirooz; Farhadi, Mohammad; Tehran, E Amin

doi:10.1016/j.yexmp.2006.04.006

Cited by 80 publications

(70 citation statements)

References 6 publications

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“…The second prevalent HPV type detected in Moroccan NPC biopsies is HPV59 (16,7%). Of interest, HPV-16 and -18, which are the most virulent genotypes associated with CC in Moroccan woman (35% to 45 %) (Khair et al 2009), were detected in very few NPC cases (8.3 %), and similar data were reported in an Iranian study (Mirzamani et al 2006). However, a recent study suggests that WHO-I NPC may be associated with oncogenic HPV.…”

Section: Wwwintechopencomsupporting

confidence: 76%

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

Moumad¹,

Laantri²,

Mohammed³

et al. 2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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Section: Wwwintechopencomsupporting

confidence: 76%

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

Moumad¹,

Laantri²,

Mohammed³

et al. 2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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“…EBV and HPV DNA have been detected in nasopharyngeal carcinoma samples, which suggests that EBV and HPV co-infections could be involved in the neoplastic transformation of the oral epithelium (105)(106)(107). Other studies have detected EBV and HPV co-infections in head and neck carcinomas and in HIV-positive oral hairy leukoplakia patients (108,109).…”

Section: Ebv and Hpvmentioning

confidence: 95%

Multiple Infections and Cancer: Implications in Epidemiology

Vedham

Divi

Starks

et al. 2014

Technol Cancer Res Treat

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Approximately 18% of the global cancer burden has been attributed to infectious agents, with estimates ranging from 7% in developed countries to about 22% in developing countries.Chronic infections caused by the hepatitis B and C viruses, human papilloma viruses (HPV), and Helicobacter pylori (H. pylori) are reported to be responsible for approximately 15% of all human cancers. Interestingly, although many of the infectious agents that have been associated with cancer-such as HPV, Epstein-Barr virus (EBV), and H. pylori-are highly prevalent in the world, most infected individuals do not develop cancer but remain lifelong carriers. Malignancies associated with infectious agents may result from prolonged latency as a result of chronic infections. Pathogenic infections are necessary but are not sufficient for cancer initiation or progression. Cancer initiation may require additional cofactors, including secondary infections. Therefore, in patients with chronic infection with one agent, secondary co-infection with another agent may serve as an important co-factor that may cause cancer initiation and progression. Additionally, opportunistic co-infections could significantly inhibit response to cancer treatment and increase cancer mortality. Co-infections are relatively common in areas with a high prevalence of infectious agents, especially in developing countries. These co-infections can cause an imbalance in the host immune system by affecting persistence of and susceptibility to malignant infections. Several articles have been published that focus on infectious agents and cancer. In this article, we discuss the role of infectious agents in malignancies, highlight the role of multiple/co-infections in cancer etiology, and review implications for cancer epidemiology.

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“…However, all patients with nasopharyngeal carcinoma were EBER-in situ hybridization (ISH)-positive (100%) and all healthy subjects were negative (100%) by EBER-ISH, which is recognized as a preferred method for detection of EBV infection with high specificity and sensitivity in present clinical practice. 25 Finally, with LMP1-SERS probe staining, LMP1 was positive in 33 of the 34 patients with nasopharyngeal carcinoma (97.1%) and negative in all 20 healthy subjects (100%). It is obvious that the SERS results were superior to those of conventional immunohistochemistry staining for LMP1 and consistent with those of in situ hybridization for EBER.…”

mentioning

confidence: 89%

Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering

Chen

Zheng²,

Chen³

et al. 2011

IJN

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Background:Previous studies have shown that Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is closely associated with the occurrence and development of nasopharyngeal carcinoma, and can be used as a tumor marker in screening for the disease. Here we report a new methodology based on highly specific and sensitive surface-enhanced Raman scattering (SERS) technology to detect LMP1 in nasopharyngeal tissue sections directly with no need of tedious procedures as with conventional immunohistochemistry methods. Methods: LMP1-functionalized 4-mercaptobenzoic acid (4-MBA)-labeled Au/Ag core-shell bimetallic nanoparticles were prepared first and then applied for analyzing LMP1 in formalinfixed paraffin-embedded nasopharyngeal tissue sections obtained from 34 cancer patients and 20 healthy controls. SERS spectra were acquired from a 25 × 25 spot square area on each tissue section and used to generate SERS images. Results: Data from SERS spectra and images show that this new SERS-based immunoassay detected LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections with high sensitivity and specificity. The results from the new LMP1-SERS probe method are superior to those of conventional immunohistochemistry staining for LMP1, and in excellent agreement with those of in situ hybridization for EBV-encoded small RNA (EBER). Conclusion:This new SERS technique has the potential to be developed into a new clinical tool for detection and differential diagnosis of nasopharyngeal carcinoma as well as for predicting metastasis and immune-targeted treatment of nasopharyngeal carcinoma.

show abstract

Detection of EBV and HPV in nasopharyngeal carcinoma by in situ hybridization

Cited by 80 publications

References 6 publications

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

Multiple Infections and Cancer: Implications in Epidemiology

Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering

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