Detection of Echinococcus multilocularis in foxes in The Netherlands

Giessen, J.W.B. van der; Rombout, Yvonne; Franchimont, J.H.; Limper, L; Homan, Wieger

doi:10.1016/s0304-4017(98)00263-5

Cited by 59 publications

(38 citation statements)

References 13 publications

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“…Individual findings, predominantly in the intermediate hosts and man, were recorded in Belarus, Moldova, Georgia, Azerbaijan, Armenia, Kazakhstan and Uzbekistan. Recently, the records of E. multilocularis have increased and it has been reported for the first time from countries such as the Netherlands, Belgium, Luxembourg, Italy, and countries neighbouring the Ukraine, Belarus, Poland, Czech Republic, Slovakia, Hungary and Romania (Abuladze 1964;Lukashenko 1975;Sadykhov 1978;Malczewski et al 1995Malczewski et al , 1999Eckert 1997;Bessonov 1998;Dubinský et al 1999Dubinský et al , 2001Giessen et al 1999;KoláÍová 1999;Romig et al 1999;Shimalov and Shimalov 2001;Sréter et al 2003;Deplazes et al 2004;Ionescu et al 2004;Traub et al 2005). This suggests that the distribution of this parasite has expanded.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Echinococcus multilocularis (Cestoda, Taeniidae) in red foxes (Vulpes vulpes) from Western Ukraine

Kharchenko

Kornyushin

Varodi

et al. 2008

Acta Parasitologica

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Echinococcus multilocularis was found for the first time in red foxes in the L'vivska and Volynska regions (Western Ukraine). Prevalence was 36% and intensity was 11-731 parasites. The material was identified as E. multilocularis on morphological characters. It is presumed that this infection of foxes with E. multilocularis occurred locally and, probably, can be considered as a consequence of the expansion of the range of the parasite.

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Section: Introductionmentioning

confidence: 99%

Occurrence of Echinococcus multilocularis (Cestoda, Taeniidae) in red foxes (Vulpes vulpes) from Western Ukraine

Kharchenko

Kornyushin

Varodi

et al. 2008

Acta Parasitologica

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“…Although the confidence interval of sensitivity was generally not given in other studies, these figures were calculated and included in Table IV. Based on these calculations, the sensitivity of the method was almost identical with that of intestinal scraping technique (IST) (Table IV), which is frequently used in postmortem surveys (van der Giessen et al 1999, Losson et al 2003, Vervaeke et al 2003, Duscher et al 2006, Berke et al 2008, Staubach et al 2011. As the 95% CI of sensitivity of the majority of tests is wide, the differences of sensitivities are most likely not significant in most cases (Table IV).…”

Section: Depending On Prevalence Of Infectionmentioning

confidence: 88%

Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

Széll

Sréter-Lancz

Sréter

2014

Acta Parasitologica

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Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3-33%) depending on the flotation solution used (specific gravities = 1.3-1.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74% (95% CI = 62-84%) and a specificity of 100% (95% CI = 94-100%). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78%, specificity = 100%). The sensitivity of the improved flotation was significantly higher (P < 0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.

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“…But there are many situations when investigation must be carried out in vivo (for example examination of dog populations). Therefore, there are some techniques for detection of E. multilocularis infection in definitive hosts by feaces examination: coproantigen detection by ELISA or different PCR technique (Craig et al 1995, Deplazes and Eckert 1996, Dinkel et al 1998, van der Giessen 1999, Machnicka et al 2003, Casulli et al 2005, Reiterová et al 2005, Dinkel et al 2011, Mobedi et al 2013. Among them PCR for copro-DNA detection is one of the most useful and sensitive.…”

Section: Introductionmentioning

confidence: 99%

Detection of Echinococcus multilocularis in faeces by nested PCR with the use of diluted DNA samples

Karamon¹

2014

Polish Journal of Veterinary Sciences

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The aim of this study was to choose the optimal variant of PCR examination of faeces to detect Echinococcus multilocularis infection which would allow to reduce the influence of different inhibitors in faeces. The investigation was carried out by comparison of 3 different methods of DNA isolation from faeces and different DNA dilutions used in PCR. Thirty five intestines of red foxes were used. Small intestines were examined by the sedimentation and counting technique (SCT). Faeces were collected from the rectum for PCR and flotation. DNA were isolated with the use of 3 different methods. Two methods were dedicated for faeces: method 1 (M1) -for larger samples and method 2 (M2) -for standard samples. The third method, method 3 (M3), was not dedicated for faeces. DNA samples were tested by nested PCR in 6 variants: not diluted (1/1) and 5 diluted (1/2.5, 1/5, 1/10. 1/20, 1/40). E. multilocularis was found by SCT in 18 from 35 (51.4%) intestines. Taenia-type eggs were detected only in 20.0% of faecal samples. In PCR the highest number of positive results (45.7%) were obtained during examination of DNA isolated by M1 method, and then 40.0% and 34.3%, respectively, for M2 and M3. In some samples positive results in PCR were obtained only in diluted DNA. For example, 8 from 12 positive samples isolated by M3 method gave the PCR negative results in non-diluted DNA and positive only after dilution 1:2.5, 1:10 or 1:20. Also 3 samples isolated by methods dedicated for stool gave positive results only after DNA dilution. The investigation has revealed that in copro-PCR for detection of E. multilocularis infection additional using of diluted DNA (besides non diluted) can avoid false negative results causing by PCR inhibition. In the best method of DNA isolation (M1), the use of non diluted DNA sample together with diluted in proportion 1:10 seems to be optimal.

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Detection of Echinococcus multilocularis in foxes in The Netherlands

Cited by 59 publications

References 13 publications

Occurrence of Echinococcus multilocularis (Cestoda, Taeniidae) in red foxes (Vulpes vulpes) from Western Ukraine

Occurrence of Echinococcus multilocularis (Cestoda, Taeniidae) in red foxes (Vulpes vulpes) from Western Ukraine

Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

Detection of Echinococcus multilocularis in faeces by nested PCR with the use of diluted DNA samples

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