Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines

Ding, Hanfeng; Li, Guangxian; Xu, Lixin; Jiang, Mingsong; Li, Runfang; Wang, Wenying; Zhang, Y.; Zhang, Xiaodong; Yao, Fangyin

doi:10.1134/s102144371001019x

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Among the agronomic traits, HD is one of the most important traits and varies widely in rice; thus, cultivars are bred for specific geographical regions and seasons (Ding et al 2010). Clear making of genetic basis affecting HD is important for different cultivation areas and crop seasons of the adaptation of rice.…”

Section: Discussionmentioning

confidence: 99%

Fine Mapping of qHD8-1, a QTL Controlling the Heading Date, to a 26-kb DNA Fragment in Rice (Oryza sativa L.)

et al. 2011

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Heading date is one of the importance agronomic traits. A library consisting of 1,123 single segment substitution lines (SSSLs) in the same genetic background of an elite rice variety Huajingxian 74 (HJX74) was evaluated for heading date (HD). From this library, the SSSL W06-26-35-1-5-2 with the substituted interval of PSM152-PSM154-PSM155-RM25-RM547-RM72-RM404 was found having a gene, which performed stable and late heading in the different environments of Shandong and Hainan provinces. To map the gene governing heading date, the SSSL W06-26-35-1-5-2 was crossed with the recipient HJX74 to develop an F 2 segregating population. The distribution of late and early heading plants in this population fitted a segregation ratio of 3:1, indicating the late heading was controlled by a dominant gene. The gene locus for heading date was tentatively designated as qHD8-1. Using a random sample of 460 individuals from the F 2 population, the qHD8-1 was narrowed down to a region flanking by two SSR markers PSM155 and RM547. For fine mapping of qHD8-1, a large F 2:3 segregating population of 3,000 individuals were developed from F 2 plants heterozygous in the PSM155-RM547 region. Recombinants analysis further mapped qHD8-1 to an interval of region 26 kb with markers RM22492 and P23 bounded on the left and right sides, respectively. Sequence analysis of this 26-kb fragment revealed that it contains five putative open reading frames, which were regarded as candidates of qHD8-1. These results will be useful in cloning of the qHD8-1 gene.

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Section: Discussionmentioning

confidence: 99%

Fine Mapping of qHD8-1, a QTL Controlling the Heading Date, to a 26-kb DNA Fragment in Rice (Oryza sativa L.)

et al. 2011

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Effects of stacked quantitative resistances to downy mildew in lettuce do not simply add up

Böer¹,

Pelgrom²,

Zhang³

et al. 2014

Theor Appl Genet

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In a stacking study of eight resistance QTLs in lettuce against downy mildew, only three out of ten double combinations showed an increased resistance effect under field conditions. Complete race nonspecific resistance to lettuce downy mildew, as observed for the nonhost wild lettuce species Lactuca saligna, is desired in lettuce cultivation. Genetic dissection of L. saligna's complete resistance has revealed several quantitative loci (QTL) for resistance with field infection reductions of 30-50 %. To test the effect of stacking these QTL, we analyzed interactions between homozygous L. saligna CGN05271 chromosome segments introgressed into the genetic background of L. sativa cv. Olof. Eight different backcross inbred lines (BILs) with single introgressions of 30-70 cM and selected predominately for quantitative resistance in field situations were intercrossed. Ten developed homozygous lines with stacked introgression segments (double combinations) were evaluated for resistance in the field. Seven double combinations showed a similar infection as the individual most resistant parental BIL, revealing epistatic interactions with 'less-than-additive' effects. Three double combinations showed an increased resistance level compared to their parental BILs and their interactions were additive, 'less-than-additive' epistatic and 'more-than-additive' epistatic, respectively. The additive interaction reduced field infection by 73 %. The double combination with a 'more-than-additive' epistatic effect, derived from a combination between a susceptible and a resistant BIL with 0 and 30 % infection reduction, respectively, showed an average field infection reduction of 52 %. For the latter line, an attempt to genetically dissect its underlying epistatic loci by substitution mapping did not result in smaller mapping intervals as none of the 22 substitution lines reached a similar high resistance level. Implications for breeding and the inheritance of L. saligna's complete resistance are discussed.

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Fine mapping of a quantitative trait locus qHD3-1, controlling the heading date, to a 29.5-kb DNA fragment in rice

Wang

Liu

Ding

et al. 2011

Russ J Plant Physiol

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Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines

Cited by 3 publications

References 22 publications

Fine Mapping of qHD8-1, a QTL Controlling the Heading Date, to a 26-kb DNA Fragment in Rice (Oryza sativa L.)

Fine Mapping of qHD8-1, a QTL Controlling the Heading Date, to a 26-kb DNA Fragment in Rice (Oryza sativa L.)

Effects of stacked quantitative resistances to downy mildew in lettuce do not simply add up

Fine mapping of a quantitative trait locus qHD3-1, controlling the heading date, to a 29.5-kb DNA fragment in rice

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