Detection of Extremely Low Concentrations of Biological Substances Using Near-Field Illumination

Yasuura, Masato; Fujimaki, Makoto

doi:10.1038/srep39241

Cited by 12 publications

(9 citation statements)

References 28 publications

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“…Additionally, an external force-assisted near-field illumination biosensor (EFA-NI biosensor) was created for HuNoV. Coated with cross-reactive mAbs, the EFA-NI biosensor recognized as few as 40 viral particles [196], suggesting that these rapid, ultra-sensitive immunoassays may complement standard nucleic acid-based methods in clinical settings.…”

Section: Applications Of Norovirus Epitope Studiesmentioning

confidence: 99%

The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics

Sels

Green

2019

Viruses

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Human norovirus (HuNoV) is the leading cause of acute nonbacterial gastroenteritis. Vaccine design has been confounded by the antigenic diversity of these viruses and a limited understanding of protective immunity. We reviewed 77 articles published since 1988 describing the isolation, function, and mapping of 307 unique monoclonal antibodies directed against B cell epitopes of human and murine noroviruses representing diverse Genogroups (G). Of these antibodies, 91, 153, 21, and 42 were reported as GI-specific, GII-specific, MNV GV-specific, and G cross-reactive, respectively. Our goal was to reconstruct the antigenic topology of noroviruses in relationship to mapped epitopes with potential for therapeutic use or inclusion in universal vaccines. Furthermore, we reviewed seven published studies of norovirus T cell epitopes that identified 18 unique peptide sequences with CD4- or CD8-stimulating activity. Both the protruding (P) and shell (S) domains of the major capsid protein VP1 contained B and T cell epitopes, with the majority of neutralizing and HBGA-blocking B cell epitopes mapping in or proximal to the surface-exposed P2 region of the P domain. The majority of broadly reactive B and T cell epitopes mapped to the S and P1 arm of the P domain. Taken together, this atlas of mapped B and T cell epitopes offers insight into the promises and challenges of designing universal vaccines and immunotherapy for the noroviruses.

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Section: Applications Of Norovirus Epitope Studiesmentioning

confidence: 99%

The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics

Sels

Green

2019

Viruses

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“…A magnetic near-field enforced illumination biosensor is developed to detect noroviruses in contaminated water using two different antibodies 122 . One antibody is linked to polystyrene beads and the other antibody is linked to magnetic beads to generate a sandwich when binding to viruses.…”

Section: Other Advanced Technologies In Poc Of Foodborne Virusesmentioning

confidence: 99%

Recent Advances in Biosensor Development for Foodborne Virus Detection

Neethirajan¹,

Ahmed²,

Chand³

et al. 2017

Nanotheranostics

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Outbreaks of foodborne diseases related to fresh produce have been increasing in North America and Europe. Viral foodborne pathogens are poorly understood, suffering from insufficient awareness and surveillance due to the limits on knowledge, availability, and costs of related technologies and devices. Current foodborne viruses are emphasized and newly emerging foodborne viruses are beginning to attract interest. To face current challenges regarding foodborne pathogens, a point-of-care (POC) concept has been introduced to food testing technology and device. POC device development involves technologies such as microfluidics, nanomaterials, biosensors and other advanced techniques. These advanced technologies, together with the challenges in developing foodborne virus detection assays and devices, are described and analysed in this critical review. Advanced technologies provide a path forward for foodborne virus detection, but more research and development will be needed to provide the level of manufacturing capacity required.

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“…Therefore, fast and reliable biosensing methods are strongly needed, and some novel techniques have already been reported. Biosensors for norovirus detection that have been proposed to date are based on electrochemical [8][9][10][11] and optical [12,13] transducers. Recently, the utilization of microfluidic devices was demonstrated for aptamer-based fluorometric and electrochemical detection [14,15].…”

Section: Introductionmentioning

confidence: 99%

Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

Janczuk-Richter

Gromadzka

Richter

et al. 2020

Sensors

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Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.

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Detection of Extremely Low Concentrations of Biological Substances Using Near-Field Illumination

Cited by 12 publications

References 28 publications

The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics

The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics

Recent Advances in Biosensor Development for Foodborne Virus Detection

Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

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