1998
DOI: 10.1007/s002530051286
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Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli

Abstract: The production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected in an agarplate assay. The assay involves the substitution of the main carbon source in specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains, were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli, Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The enzyme was released from harvested cells by sonica… Show more

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Cited by 122 publications
(102 citation statements)
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“…FAE activity was identified in several lactobacilli, including L. fermentum, L. reuteri, L. leichmanni, and L. farciminis, however the genes encoding the FAEs were not identified (Donaghy et al, 1998). It is hypothesized that probiotic bacteria could enhance the release of bioactive phenolic acids from dietary fiber by producing the necessary FAE activity and aiding in the assimilation of phenolic acids.…”
Section: Identification Of Feruloyl Esterases From Bacteriamentioning
confidence: 99%
See 1 more Smart Citation
“…FAE activity was identified in several lactobacilli, including L. fermentum, L. reuteri, L. leichmanni, and L. farciminis, however the genes encoding the FAEs were not identified (Donaghy et al, 1998). It is hypothesized that probiotic bacteria could enhance the release of bioactive phenolic acids from dietary fiber by producing the necessary FAE activity and aiding in the assimilation of phenolic acids.…”
Section: Identification Of Feruloyl Esterases From Bacteriamentioning
confidence: 99%
“…A screening assay for detection of FAE activity from Lactobacillus strains was first described by Donaghy et al (Donaghy et al, 1998). A model substrate for FAEs, ethyl ferulate, was embedded in de Man Rogosa Sharpe (MRS) plates.…”
Section: Identification Of Fae-producing Bacterial Strainsmentioning
confidence: 99%
“…Secondary screening for feruloyl esterase activities was performed on ethyl ferulate agar assay as essentially described by Donaghy et al (21). Quantitative assays: All quantitative assays were performed in triplicate.…”
Section: Methodsmentioning
confidence: 99%
“…A total of three feruloyl esterase positive fosmid clones were identified in this study on ethyl ferulate agar plates (21). To identify feruloyl esterase open reading frame (ORF) in one of the active fosmid clones, a pUC19 subclone library was generated from a partially digested Sau3AI fosmid DNA.…”
Section: Library Screeningmentioning
confidence: 99%
“…Positive esterase clones were screened for ferulic acid esterase activity by plating onto basal agar supplemented with 0.4% (w/v) ethyl 4-hydroxy-3 methoxycinnamate (ethyl ferulate) (Sigma). A clear halo surrounding a colony indicated ferulic acid esterase activity [19]. [20].…”
Section: High-throughput Screening Of Fosmid Library Clonesmentioning
confidence: 99%