Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test

Nüesch‐Inderbinen, Magdalena; Hächler, Herbert; Kayser, Fritz H.

doi:10.1007/bf01690097

Cited by 211 publications

(148 citation statements)

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“…Similarly, low transconjugation success was also reported by other workers in Switzerland and Germany [19,34]. Franiczek et al [35] also reported that none of the four E. coli isolates could transfer their resistance gene to other recipient strains.…”

Section: Discussionmentioning

confidence: 55%

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

et al. 2013

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Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extendedspectrum b-lactamases (ESBLs). A total of 138 enteric bacteria were isolated from 53 faecal samples of pigs collected from different districts of Mizoram, of which 102 (73.91 %) were Escherichia coli, 26 (18.84 %) were Salmonella spp. and 10 (7.25 %) were Klebsiella pneumoniae. Phenotypic confirmatory test (Double Discs Synergy Test) showed that 8 (5.80 %) E. coli isolates were ESBLs producer. PCR analysis confirmed that out of the eight isolate, 7 (5.07 %) harboured bla CTX-M-1 gene and/or bla TEM gene. Of the eight positive isolates, 7 (5.07 %) and 3 (2.17 %) were found to be positive for bla CTX-M-1 gene and bla TEM gene, respectively, of which 3 (2.17 %) isolates were positive for both the genes. Only 4 (2.90 %) E. coli isolates carried bla CTX-M-1 gene alone. Agarose gel electrophoresis showed that all the isolates were carrying plasmids ranging between 0.9 and *30 kb. Out of the seven isolates positive for bla CTX-M-1 and/or bla TEM , 2 (1.84 %) isolates were confirmed for bla CTX-M-1 gene in their plasmid. Only one E. coli isolate was found to be positive for both the genes in its plasmid. The resistance plasmid could not be transferred to a recipient by in vitro horizontal gene transfer method.

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Section: Discussionmentioning

confidence: 55%

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

et al. 2013

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“…Some ESBLs may fail to reach a level to be detected by disk diffusion tests but result in treatment failure in the infected patient. Nuesch-Inderbinen and Hachler (1996) reported that molecular methods appear sensitive, are expensive, time consuming and require specialized equipment and expertise. However, definitive identification is possible only by molecular detection methods.…”

Section: Discussionmentioning

confidence: 99%

First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum -lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina Faso

Zongo¹,

Dabiré²,

Compaore³

et al. 2015

Afr. J. Biotechnol.

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Resistance to a wide variety of common antimicrobials is observed among clinical strains designed as extended spectrum β-lactmase (ESBL) producers. They produce enzymatic protein which inactivates efficiently oxyimino cephalosporin and constitutes a serious global health concern that has complicated treatment strategies. Many studies report high prevalence of ESBL producers among Gram negative bacilli. The aim of this work was to identify the presence of TEM, SHV and CTX-M families in these strains which were initially screened by phenotypic method. Gram negative bacilli resisting third or four generation cephalosporin were isolated during anti-biogram study. The presence of ESBL positivity was detected using the double disk synergy test. Minimal inhibitory concentrations (MICs) of ceftriazon for any strain were determined using E-test manufacturing protocol. Polymerase chain reaction (PCR) analysis for β-lactamase (bla) genes of TEM, SHV and CTX-M family was carried out using designed primers in 171 ESBL isolates producers. Among 259 Gram negative bacilli collected, 171 (66, 02%) exhibited ESBL producers' profile. Urine samples constitute major source of ESBL producers. The highest prevalence of ESBL was observed in Escherichia coli (75, 50%). Among ESBL isolates producers, gene prevalence of bla-CTX-M (65, 49%) was highest, followed by bla-TEM (25, 73%) and bla-SHV (18, 71%) in the present study. The frequency of ESBL producing strains among clinical isolates has been steadily increased. Continual drug resistance surveillance and molecular characteristics of ESBL isolates are necessary to guide the appropriate and judicious antibiotic use.

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“…Digestion of bla SHV with NheI was carried out in order to identify Gly238→Ser mutation associated with the hydrolysis of third generation cephalosporins [19]. Sequencing of bla CTX-M and bla TEM genes were performed for single representatives of major clones [17,20].…”

Section: Detection Of β-Lactamase Genesmentioning

confidence: 99%

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Melegh

Schneider

Horváth

et al. 2015

Acta Microbiologica et Immunologica Hungarica

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We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period [2004][2005][2006][2007][2008]. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla CTX-M , bla TEM and bla SHV ) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fi mbria, biofi lm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofl oxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identifi ed, and were shown to harbour the following β-lactamase genes: six clones carried bla CTX-M , four clones harboured bla SHV-5 and one clone possessed both bla CTX-M and ESBL type bla SHV . Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profi le were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofl oxacin, minor clone *Corresponding author; E-mail: melegh.szilvia@pte.hu 234 MELEGH et al.Acta Microbiologica et Immunologica Hungarica 62, 2015 isolates displayed signifi cantly lower MIC values for ciprofl oxacin suggesting a role for fl uoroquinolones in the dissemination of the major K. pneumoniae clones. This is the fi rst description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary.

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Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test

Cited by 211 publications

References 23 publications

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum -lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina Faso

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

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