Detection of Huanglongbing (citrus greening disease) in Timor-Leste (East Timor) and in Papua New Guinea

Weinert, M.; Jacobson, S. C.; Grimshaw, J. F.; Bellis, Glenn; Stephens, P. M.; Gunua, T. G.; Kame, M. F.; Davis, R. I.

doi:10.1071/ap03089

Cited by 35 publications

(21 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. asiaticus' perhaps originated in India. From the 20 th century onward, HLB spread through much of the citrus-growing regions of south and southeast Asia [2], the Arabian peninsula [31], East Timor and Papua New Guinea [32], and the western hemisphere (Brazil and the United States) [1]. It is difficult to precisely know when the disease entered each country and from where it was introduced.…”

Section: Discussionmentioning

confidence: 99%

Multilocus microsatellite analysis of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing worldwide

et al. 2012

View full text Add to dashboard Cite

BackgroundHuanglongbing (HLB) is one of the most destructive citrus diseases in the world. The disease is associated with the presence of a fastidious, phloem-limited α- proteobacterium, 'Candidatus Liberibacter asiaticus', 'Ca. Liberibacter africanus' or 'Ca. Liberibacter americanus'. HLB-associated Liberibacters have spread to North America and South America in recent years. While the causal agents of HLB have been putatively identified, information regarding the worldwide population structure and epidemiological relationships for 'Ca. L. asiaticus' is limited. The availability of the 'Ca. L. asiaticus' genome sequence has facilitated development of molecular markers from this bacterium. The objectives of this study were to develop microsatellite markers and conduct genetic analyses of 'Ca. L. asiaticus' from a worldwide collection. Two hundred eighty seven isolates from USA (Florida), Brazil, China, India, Cambodia, Vietnam, Taiwan, Thailand, and Japan were analyzed.ResultsA panel of seven polymorphic microsatellite markers was developed for 'Ca. L. asiaticus'. Microsatellite analyses across the samples showed that the genetic diversity of 'Ca. L. asiaticus' is higher in Asia than Americas. UPGMA and STRUCTURE analyses identified three major genetic groups worldwide. Isolates from India were genetically distinct. East-southeast Asian and Brazilian isolates were generally included in the same group; a few members of this group were found in Florida, but the majority of the isolates from Florida were clustered separately. eBURST analysis predicted three founder haplotypes, which may have given rise to three groups worldwide.ConclusionsOur results identified three major genetic groups of 'Ca. L. asiaticus' worldwide. Isolates from Brazil showed similar genetic makeup with east-southeast Asian dominant group, suggesting the possibility of a common origin. However, most of the isolates recovered from Florida were clustered in a separate group. While the sources of the dominant 'Ca. L. asiaticus' in Florida were not clearly understood, the less-pervasive groups may have been introduced directly from Asia or via Brazil. Notably, the recent outbreak of HLB in Florida probably occurred through multiple introductions. Microsatellite markers developed in this study provide adequate discriminatory power for the identification and differentiation of closely-related isolates, as well as for genetic studies of 'Ca. L. asiaticus'.

show abstract

Section: Discussionmentioning

confidence: 99%

Multilocus microsatellite analysis of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing worldwide

et al. 2012

View full text Add to dashboard Cite

show abstract

“…HLB has moved west from Pakistan into Iran (Faghihi et al ), threatening Turkey and beyond, and the African psyllid recently was found in Spain (Pérez‐Otero et al ). HLB was detected in Papua New Guinea in 2002 (Weinert et al ) causing Australia to step up its vigilance.…”

Section: Introductionmentioning

confidence: 99%

Huanglongbing: An overview of a complex pathosystem ravaging the world's citrus

Graça

Douhan

Halbert

et al. 2015

JIPB

263

154

View full text Add to dashboard Cite

Citrus huanglongbing (HLB) has become a major disease and limiting factor of production in citrus areas that have become infected. The destruction to the affected citrus industries has resulted in a tremendous increase to support research that in return has resulted in significant information on both applied and basic knowledge concerning this important disease to the global citrus industry. Recent research indicates the relationship between citrus and the causal agent of HLB is shaped by multiple elements, in which host defense responses may also play an important role. This review is intended to provide an overview of the importance of HLB to a wider audience of plant biologists. Recent advances on host-pathogen interactions, population genetics and vectoring of the causal agent are discussed.

show abstract

“…HLB disease has a long incubation period and symptom-less in certain host plants, but infected citrus plants occur in fi elds. Another diffi culty is that HLB symptoms resemble symptoms of plant nutrient defi ciency or disorders, so it is not easily diagnosed (Weinert et al 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Nutritional Programme: An Innovative Approach to Controlling Plant Diseases in the Tropics

Ahmad

Ismail

2016

Plant, Soil and Microbes

View full text Add to dashboard Cite

Detection of Huanglongbing (citrus greening disease) in Timor-Leste (East Timor) and in Papua New Guinea

Cited by 35 publications

References 3 publications

Multilocus microsatellite analysis of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing worldwide

Multilocus microsatellite analysis of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing worldwide

Huanglongbing: An overview of a complex pathosystem ravaging the world's citrus

Enhanced Nutritional Programme: An Innovative Approach to Controlling Plant Diseases in the Tropics

Contact Info

Product

Resources

About