Detection of
            <i>mcr-1</i>
            Gene among Escherichia coli Isolates from Farmed Fish and Characterization of
            <i>mcr-1</i>
            -Bearing IncP Plasmids

Lv, Luchao; Cao, Yuping; Yu, Peixin; Huang, Raorao; Wang, Jing; Wen, Qiaoling; Zhi, Chanping; Zhang, Qianhui; Liu, Jianhua

doi:10.1128/aac.02378-17

Cited by 28 publications

(25 citation statements)

References 24 publications

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“…Furthermore, this sequence type has been associated with the global carriage of ESBL-producing E. coli [13,34,48]. E. coli ST156 was identified in fish [50], canines, felines, horses [51], chickens, and other avian species [52,53]. Lastly, this study is the first demonstration of ST1380 E. coli being isolated from equines, the sequence type that was previously isolated from swine and bovine species [54,55].…”

Section: Discussionmentioning

confidence: 55%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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Simple Summary: The spread and development of extended spectrum beta-lactamase (ESBL)-mediated antimicrobial resistance is a significant concern in healthcare with impacts to animal and public health alike. While the occurrence of the ESBL phenotype in Escherichia coli has been investigated in depth by numerous studies, there is still a lack of information regarding ESBL-producing bacterial isolates from clinical specimens of equine origin. In this study, we investigated the incidence of ESBL-producing E. coli in hospitalized horses. Overall, 207 E. coli isolates were analyzed for their antimicrobial susceptibility and 13 ESBL-producing E. coli isolates were genotypically characterized. Seven out of the 13 E. coli isolates were found to harbor the resistance genes bla CTX-M-1 or bla SHV-1 and a novel beta-lactamase TEM gene variant, bla TEM-233 was discovered. Furthermore, despite being phenotypically susceptible to tested carbapenems, 1 out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 . The latter is an alarming finding because the presence of carbapenemase resistance genes in equine pathogens is extremely rare. In conclusion, equines can be reservoirs for ESBL-producing Enterobacteriaceae, and further investigation into this species group is necessary to understand their impact in the spread and development of antibiotic resistance genes.Abstract: Escherichia coli isolates were recovered from clinical specimens of equine patients admitted to the Texas A&M Veterinary Medical Teaching Hospital over a five-year period. Ceftiofur resistance was used as a marker for potential extended-spectrum beta-lactamase (ESBL)-activity, and of the 48 ceftiofur-resistant E. coli isolates, 27.08% (n = 13) were phenotypically ESBL-positive. Conventional PCR analysis followed by the large-scale bla Finder multiplex PCR detected the ESBL genes, CTX-M-1 and SHV, in seven out of the 13 isolates. Moreover, beta-lactamase genes of TEM-1-type, BER-type (AmpC), and OXA-type were also identified. Sequencing of these genes resulted in identification of a novel TEM-1-type gene, called bla TEM-233 , and a study is currently underway to determine if this gene confers the ESBL phenotype. Furthermore, this report is the first to have found E. coli ST1308 in horses. This subtype, which has been reported in other herbivores, harbored the SHV-type ESBL gene. Finally, one out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 despite the lack of phenotypically proven resistance to imipenem. With the identification of novel ESBL gene variant and the demonstrated expansion of E. coli sequence types in equine patients, this study underscores the need for more investigation of equines as reservoirs for ESBL-producing pathogens. ]. The wide application of beta-lactam antibiotics has been considered as a driving factor in the development and spread of extended-spectrum beta-lactamase (ESBL)-conferred resistance in Gram-negative bacterial pathogens such as Escherichia coli, Klebsiella spp., and Sa...

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Section: Discussionmentioning

confidence: 55%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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“…MLST analysis of the sequenced genomes showed that F1 and F2 belonged to sequence types, ST48 and ST101, respectively. These STs have been associated with mcr and multidrug resistant strains and occur in a variety of niches and hosts, including humans, avian hosts, and fish [ 23 , 64 , 65 , 66 ]. This highlighted the ability of mcr-1 to spread to important matrices and hosts and suggested that F1 and F2 isolates can be transmitted beyond the trout farm and/or can be acquired by the fish from other polluted sources [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…In a study conducted on integrated fish-duck farming, it was suggested that E. coli harboring the mcr-1 can spread from aquatic animals to affect the supply chain and humans [ 24 ]. Therefore, the detection of mcr-1.1 in fish poses a significant public health concern, because resistance can be potentially transmitted to pathogens that affect humans and other animals [ 23 , 24 ]. Additionally, international trade with farmed fish can act as a route for the dissemination of mcr between countries [ 18 , 68 ].…”

Section: Resultsmentioning

confidence: 99%

“…It was proposed that aquatic systems are a significant reservoir for colistin-resistant genes and can transmit them directly and indirectly to humans [ 22 ]. Furthermore, seven and two mcr-1 -positive E. coli were recovered from grass carp fish farms and fish in integrated fishery operations in Guangzhou in Southern China [ 23 , 24 ]. In Vietnam, mcr-1 was detected in one extended-spectrum beta-lactamase (ESBL)-producing E. coli isolated from striped catfish grown in ponds [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…A recent study from Spain reported mcr-1 in Salmonella enterica serovar Rissen isolated from mussels [ 27 ]. However, beyond these few studies on farmed fish in China and Vietnam and on mussels in Spain [ 23 , 24 , 25 , 26 , 27 ], data on the occurrence of mcr in aquaculture have been limited. Therefore, it is important to investigate colistin resistance and mcr in aquaculture globally, especially in developing countries with limited infrastructure and documented challenges in pollution.…”

Section: Introductionmentioning

confidence: 99%

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The Mobile Colistin Resistance Gene, mcr-1.1, Is Carried on IncX4 Plasmids in Multidrug Resistant E. coli Isolated from Rainbow Trout Aquaculture

et al. 2020

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Colistin, a last resort antibiotic, is important for controlling infections with carbapenem-resistant Enterobacteriaceae. The recent emergence of mobile-colistin-resistance (mcr) genes has threatened the effectiveness of colistin. Aquaculture is hypothesized to be a major contributor to the evolution and dissemination of mcr. However, data on mcr in aquaculture are limited. Here, the occurrence of mcr-1 was assessed in Rainbow Trout in Lebanon, a country with developing antimicrobial stewardship and an established use of colistin for medical and farming purposes. mcr-1 was detected in 5 Escherichia coli isolated from fish guts. The isolates were classified as multidrug-resistant and their colistin minimum inhibitory concentration ranged between 16 and 32 μg/mL. Whole genome sequencing analysis showed that mcr-1 was carried on transmissible IncX4 plasmids and that the isolates harbored more than 14 antibiotic resistance genes. The isolates belonged to ST48 and ST101, which have been associated with mcr and can occur in humans and fish. The mcr-1-positive E. coli persisted in 6-day biofilms, but there was a potential fitness cost. Given the status of infrastructure in Lebanon, there is a high potential for the dissemination of mcr via aquatic environments. Urgent actions are needed to control mcr and to enhance antimicrobial stewardship in Lebanon.

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Colistin Resistance in Aquaculture Environment: An Emerging Challenge

Dwivedi

Awasthi

2023

Macromolecular Symposia

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The emergence of colistin resistance is one of the most serious future issues since it is a last‐resort antibiotic used to treat infections caused by multidrug‐resistant (MDR) gram‐negative bacteria, particularly those resistant to carbapenems. The aquatic environment acts as a reservoir for the emergence and spread of colistin resistant pathogens. The emergence and transmission of a plasmid mediated mcr gene is one of the most important mechanism of colistin resistance in gram negative bacteria. Several mcr variants have been identified in zoonotic pathogens of aquatic origin that may easily transmit this gene to human pathogens. Currently, very few studies have been conducted to test colistin susceptibility in aquaculture associated bacteria. This may be attributed to a limited technical resource for screening colistin‐resistant microorganisms. As a result, to reduce the emergence and spread of colistin resistance in human and animal health, as well as in the environment, the One Health strategy and laboratory methodologies must be expanded.

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Detection of mcr-1 Gene among Escherichia coli Isolates from Farmed Fish and Characterization of mcr-1 -Bearing IncP Plasmids

Cited by 28 publications

References 24 publications

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

The Mobile Colistin Resistance Gene, mcr-1.1, Is Carried on IncX4 Plasmids in Multidrug Resistant E. coli Isolated from Rainbow Trout Aquaculture

Colistin Resistance in Aquaculture Environment: An Emerging Challenge

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