2003
DOI: 10.1128/aem.69.10.6243-6249.2003
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Detection of Prochlorothrix in Brackish Waters by Specific Amplification of pcb Genes

Abstract: Prochlorothrix hollandica is the only filamentous chlorophyll b (Chlb)-containing oxyphotobacterium that has been found in freshwater habitats to date. Chlb serves as a light-harvesting pigment which is bound to special binding proteins (Pcb). Even though Prochlorothrix was initially characterized as a highly salt-sensitive species, we detected it in a brackish water environment that is characterized by salinities of up to 12 practical salinity units. Using PCR and reverse transcription, we amplified pcb gene … Show more

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Cited by 14 publications
(13 citation statements)
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“…Interestingly, although the filamentous Prochlorothrix is known to be a genus sensitive to high salinity, the uncommon species P. hollandica has been found in the Baltic Sea, in an environment with salinity up to 12 psu (BurgerWiersma et al, 1989;Geiss et al, 2003). In Sweden and in the Netherlands particularly, the most commonly reported cyanobacteria have been Microcystis aeruginosa, Aphanizomenon flosaquae, Oscillatoria spp., Planktothrix spp.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Interestingly, although the filamentous Prochlorothrix is known to be a genus sensitive to high salinity, the uncommon species P. hollandica has been found in the Baltic Sea, in an environment with salinity up to 12 psu (BurgerWiersma et al, 1989;Geiss et al, 2003). In Sweden and in the Netherlands particularly, the most commonly reported cyanobacteria have been Microcystis aeruginosa, Aphanizomenon flosaquae, Oscillatoria spp., Planktothrix spp.…”
Section: Introductionmentioning
confidence: 99%
“…E-mail: vmvascon@ fc.up.pt Tan, 2007). The cyanobacteria of brackish waters (including estuaries), however, remain under-investigated, particularly in Europe, despite a number of significant contributions (Sivonen et al, 1989;Nehring, 1993;Lehtima¨ki et al, 1994Lehtima¨ki et al, , 1997Lehtima¨ki et al, , 2000Wille´n & Mattson, 1997;Sivonen & Jones, 1999;Sipia¨et al, 2001Sipia¨et al, , 2002Sipia¨et al, , 2006Sipia¨et al, , 2007Sipia¨et al, , 2008Vasconcelos & Cerqueira, 2001;Laamanen et al, 2002;Rocha et al, 2002;Geiss et al, 2003;Hobson & Fallowfield, 2003;Lehtonen et al, 2003;Stal et al, 2003;Sobrino et al, 2004;Herfindal et al, 2005;Kankaanpa¨a¨et al, 2005;Karjalainen et al, 2005Karjalainen et al, , 2007Karlsson et al, 2005;Pere´z & Carrilo, 2005;Surakka et al, 2005;Marino et al, 2006;Mazur-Marzec et al, 2007Paoli et al, 2007;Halinen et al, 2008;Ibelings & Havens, 2008;Jo´z´wiak et al, 2008;Lopes et al, 2010;Oftedal et al, 2010;). This lack of information for brackish-water cyanobacteria is especially significant in relation to the potential for production of new bioactive compounds.…”
Section: Introductionmentioning
confidence: 99%
“…Prochlorothrix hollandica and pcbC gene detection: To detect the presence of Prochlorothrix hollandica, the DNA extracted from each environmental sample was submitted to a PCR amplification using the 16S-Pholl-fw/16S-Pholl-rev primer pair that specifically targets the 16S rRNA gene of P. hollandica (Geiss et al 2003). In addition, PCR detection of the gene pcbC of the operon encoding the chl a/b binding protein was carried out using primers pcbC-fw and pcbCrev as described by Geiss et al (2003) to detect chl bcontaining oxyphotobacteria.…”
Section: Methodsmentioning
confidence: 99%
“…In addition, PCR detection of the gene pcbC of the operon encoding the chl a/b binding protein was carried out using primers pcbC-fw and pcbCrev as described by Geiss et al (2003) to detect chl bcontaining oxyphotobacteria.…”
Section: Methodsmentioning
confidence: 99%
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