Detection of increased tissue concentrations of nerve growth factor with an improved extraction procedure

Zettler, C.; Bridges, D.; Zhou, Xin‐Fu; Rush, Robert A.

doi:10.1002/(sici)1097-4547(19961201)46:5<581::aid-jnr7>3.0.co;2-f

Cited by 62 publications

(33 citation statements)

References 65 publications

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“…However, this study did not determine NGF levels in the primary sensory neurons after ischemia. In our current study, the ELISA method was used to assess NGF levels in DRG neurons as reported previously (53). The femoral artery occlusion elevated NGF in the DRG, and 24 and 48 h of postocclusion significantly increased NGF levels in the DRG of ischemic rats.…”

Section: Discussionsupporting

confidence: 88%

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Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion

Xing¹,

Lu²,

Li³

2009

American Journal of Physiology-Heart and Circulatory Physiology

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In rats, hindlimb muscle ischemia induced by femoral artery occlusion augments the sympathetic nervous response to stimulation of transient receptor potential vanilloid type 1 (TRPV1) by injection of capsaicin into the arterial blood supply of the hindlimb muscles. The enhanced sympathetic response is due to alterations in TRPV1 receptor expression and its responsiveness in sensory neurons. The underlying mechanism by which TRPV1 receptor responses are increased after muscle vascular insufficiency/ischemia is unclear. In this report we tested the hypothesis that muscle ischemia elevates nerve growth factor (NGF) levels in primary afferent neurons, thereby increasing TRPV1 responsiveness. Muscle vascular insufficiency induced by the femoral artery ligation significantly increased NGF in the dorsal root ganglion (DRG) compared with sham controls. Furthermore, when NGF was infused in the hindlimb muscles of healthy rats (72 h using an osmotic minipump), the magnitude of the DRG neuron response to capsaicin was augmented (5.4 +/- 0.54 nA with NGF infusion vs. 3.0 +/- 0.17 nA in control; P < 0.05). With the addition of NGF in the culture dish containing the DRG neurons, the magnitude of the DRG neuron response to capsaicin was greater (6.4 +/- 0.27 nA; P < 0.05 vs. control) than that seen in control (2.9 +/- 0.16 nA). Note that this NGF effect was seen in isolectin B(4)-negative DRG neurons, a group of thin fiber nerves that contain neuropeptides and depend on NGF for survival. These data suggest that NGF affects a selective subpopulation of the afferent neurons in mediating augmented TRPV1 responses after femoral artery occlusion.

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Section: Discussionsupporting

confidence: 88%

“…We chose to harvest the L4-L6 DRGs because they largely innervate the hindlimb muscles studied in this experiment. NGF levels were determined using a two-site immunoenzymatic assay (ELISA) as previously described (53). It is noted that the ELISA measurements were made on L4-L6 DRGs from individual animals.…”

Section: Femoral Artery Ligationmentioning

confidence: 99%

Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion

Xing¹,

Lu²,

Li³

2009

American Journal of Physiology-Heart and Circulatory Physiology

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“…The homogenized tissue was centrifuged for 20 min at 14,000 rpm. The NGF Emax Immunoassay system ELISA-kit (Promega, Mannheim, Germany) was employed on the extracted supernatent according to the protocol provided by the manufacturer (Zettler et al 1996). We performed measurements in pilot experiments on normal skin by taking different pieces of adjacent back skin.…”

Section: Evaluation Of Ngf Contentmentioning

confidence: 42%

Increase in NGF content and nerve fiber sprouting in human allergic contact eczema

Kinkelin¹,

Mötzing

Koltenzenburg

et al. 2000

Cell and Tissue Research

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There is increasing evidence for an intimate interaction of the skin and the nervous system. As known from animal studies, nerve growth factor (NGF) is essential for the innervation density and functional properties of sensory neurons of the skin during embryogenesis and in adulthood, and possibly during cutaneous inflammation. This study examined NGF content and sprouting of nerves during the elicitation phase of contact allergy in human skin. Skin biopsies from patients (n=14) undergoing patch-testing were taken from positive test sites and control back skin 96 h after antigen application. NGF content was measured by enzyme-linked immunofluorescence assay. Immunohistochemistry was performed for protein gene product 9.5 (PGP9.5), a marker that stains all neuronal elements, and growth-associated protein 43 (GAP43), a marker for axonal growth cones. The NGF content was significantly increased in lesional skin in comparison with normal skin (4.2+/-0.6 pg to 2.9+/-0.5 pg NGF per mg wet weight). The length of epidermal PGP9.5-immunoreactive (ir) fibers in lesional skin significantly increased from 3.4+/-0.9 mm in normal skin to 5.3+/-1.0 mm in contact eczema, whereas dermal fibers were unaltered (11.1+/-2.7 mm vs 9.5+/-2.1 mm, respectively). GAP43-ir nerve endings were significantly increased in both epidermis (1.6+/-0.3 mm to 2.6+/-0.4 mm) and dermis (0.5+/-0.1 mm to 1.8+/-0.2 mm) in contact eczema. Thus, we have provided evidence for an NGF-mediated nerve-fiber sprouting in human contact eczema. This may have a functional impact on skin-associated immune cells, in particular mast cells and Langerhans cells.

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“…These results are consistent with a number of independent published findings of high NGF in the SHR strain. 3,4,[7][8][9][10]15 However, the new finding in the present study is that this increased level of NGF mRNA is associated with the inher- …”

Section: Discussionmentioning

confidence: 99%

Nerve Growth Factor Gene Locus Explains Elevated Renal Nerve Growth Factor mRNA in Young Spontaneously Hypertensive Rats

1998

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Abstract-Nerve growth factor (NGF) controls the growth of sympathetic nerves and is increased in young spontaneously hypertensive rats (SHR). The NGF gene has been linked genetically with hypertension in the SHR strain and may explain high NGF mRNA levels. To test for genetic linkage between the NGF gene and its expression in vivo, we examined renal NGF mRNA levels in male SHR, control Donryu rats (DRY), and F 2 rats derived from SHR and DRY at ages 2, 4, 10, and 20 weeks. Tail-cuff blood pressure was measured at 4, 10, and 20 weeks of age. NGF mRNA levels in SHR (NGF genotype: SS) were higher than those in DRY (NGF genotype: DD) at 2, 4, and 10 weeks of age (PϽ0.0001) but the same at 20 weeks of age. In the F 2 generation, the S allele was associated with significantly (Pϭ0.01) higher renal NGF mRNA levels at 2 weeks of age. Mean NGF mRNA levels fell (Pϭ0.01) with age in F 2 rats, and the difference between SS and DD genotype F 2 rats diminished at older ages and was not significant. In F 2 rats there was a positive correlation between the number of NGF S alleles inherited and tail-cuff pressure (PϽ0.007).

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Detection of increased tissue concentrations of nerve growth factor with an improved extraction procedure

Cited by 62 publications

References 65 publications

Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion

Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion

Increase in NGF content and nerve fiber sprouting in human allergic contact eczema

Nerve Growth Factor Gene Locus Explains Elevated Renal Nerve Growth Factor mRNA in Young Spontaneously Hypertensive Rats

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