Detection of latent tuberculosis by the tuberculin skin test and a whole-blood interferon-γ release assay, and the development of active tuberculosis in HIV-seropositive persons

IP-10 release assays in the diagnosis of tuberculosis infection: current status and future directions

“…However, many studies have shown that the IGRAs, in particular the QFT-IT, render many indeterminate and possibly also false-negative results in HIV-infected individuals with a low CD4 cell count [13,14,[65][66][67][68][69][70][71][72]. Therefore, alternative approaches in this group of patients are necessary, but only few studies have evaluated IP-10 for this purpose.…”

Diagnosis of tuberculosis infection using interferon-γ-based assays

“…Conversely, subsequent active TB in the next two to three years seems to be extremely low among people with a negative result. [85][86][87][89][90][91][92][93][94] Table 1 summarizes the nine studies assessing development of active TB with IFN-γ-based tests.…”

“…91,92 In a large study in a low-prevalence country, 8.1% of HIV-seropositive patients with a positive QFT-IT result at baseline, and left untreated, developed TB during a median follow-up of 19 months. None of the 738 patients with negative results had TB.…”

“…91 In a study in Spain of 135 HIV-infected individuals without active disease, none of the 103 patients who had a negative or indeterminate QFT-IT result at baseline had TB after a median follow-up of 20 months. 92 Development of TB was also assessed in child and adolescent contacts. Bakir et al 93 studied 908 children with recent household exposure to TB, most of whom received preventive therapy.…”

Testy IGRA w Diagnostyce Zakażenia Prątkami Gruźlicy w Wybranych Sytuacjach Klinicznych

“…Interpretacja wyników IGRA jest obiektywna i polega na pomiarze stężenia IFN-γ wydzielanego przez limfocyty T w odpowiedzi na swoiste antygeny Mycobacterium tuberculosis za pomocą techniki ELISA (enzyme-linked immunosorbent assay) lub na zliczaniu limfocytów T wydzielających cytokinę przy użyciu metody ELISpot [7,[13][14][15][16][17][18][19][20][21].…”