Detection of Non Typhoidal Salmonella Isolated from Food Products and Clinical Cases by PCR and Conventional Methods : a Comparative Study

El-Feky, Mohamed A; Hassan, Mona A.; Mohamed, Wegdan A.

doi:10.12816/0004965

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…In the current study, the Salmonella had a higher prevalence in eggshells (7.3%) compared to the other samples, while it was not recovered from the egg contents. These findings are consistent with obtained results in a study by El-Feky et al (2013) who reported a similar isolation rate (7.83%) of Salmonella from the eggshells and also they found that Salmonella was not detected from egg content. However, EL-Kholy et al (2014) reported that Salmonella could not be isolated from both the eggshell and the egg content.…”

Section: Discussionsupporting

confidence: 93%

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Diab¹,

Zaki²,

Ibrahim³

et al. 2019

jwpr

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Non-Typhoidal Salmonella (NTS) are substantial foodborne pathogens that lead to bacteremia, gastroenteritis, and focal infection. Poultry is one of the usual provenances for the development of multidrug-resistance NTS. This problem has increased in developing countries with the indiscriminate use of antibiotics in the poultry production system. The current study aimed to determine the prevalence and tendency of antimicrobial resistance of zoonotic Salmonella spp. A total of 601 samples, including cloacal samples (150) eggshell (150), egg content (15 pooled samples), layer hen carcasses (150), hand swab (68) and stool samples (68) from poultry workers, were collected from five layer chicken farms. Isolation of NTS was performed by using different cultural and biochemical methods. Moreover, Salmonella isolates were evaluated for antimicrobial susceptibility using the disc diffusion method. The cloacal samples and stool samples showed the prevalence of Salmonella spp. at approximately similar rates of 4.7% and 4.4%, respectively. Chicken isolates were identified as S. Enteritidis, S. Typhimurium, and S. Gallinarum while the human isolates were only S. Typhimurium and S. Enteritidis. The prevalence of the NTS on the surface of the eggshells (7.3%) was higher than that in the other samples. Among 12 antimicrobials tested, 86.4% resistance was found to streptomycin and oxytetracycline followed by neomycin and erythromycin (77.3%), norfloxacin and ampicillin (68.2%) across the study sites. Kanamycin and gentamicin remained sensitive by 95.5% and 90.9%, respectively. The present study indicated that layer chickens and its products are important sources for human infection with multiple-drug resistant NTS strains.

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Section: Discussionsupporting

confidence: 93%

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Diab¹,

Zaki²,

Ibrahim³

et al. 2019

jwpr

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“…Some studies reported the detection of this gene in all tested Salmonella spp. isolates [49][50][51][52][53][54][55][56][57][58]. On the other side, Moussa et al [59] and Maysa and Abd-Elall [60] couldn't find invA gene in all isolates, same as our study.…”

Section: Resultssupporting

confidence: 84%

Genetic diversity of food originated Salmonella isolates

Karatuğ

Yüksel

Akçelik

et al. 2018

Biotechnology & Biotechnological Equipment

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Currently, Salmonella enterica is the most common bacterial foodborne pathogen, causing serious extraintestinal disease. Typing methods play an important role on pathogens' source tracking, knowing the source(s) of bacteria in pharmaceutical sciences, preventing and controlling the diarrhea and food-poisoning outbreaks. The purpose of this study is to use different moleculer typing methods to determine the genetic variability of 38 foodborne Salmonella isolates that were previously identified by biochemical tests. The methods were evaluated by four molecular techniques including 16S rRNA sequencing, PFGE, PCR-RFLP and invA-spvC PCR. 16S rRNA sequencing results showed that four of the 38 isolates were Escherichia coli, Proteus mirabilis and Citrobacter murliniae, and the others were Salmonella enterica. Thirty-eight strains were subtyped by XbaI-PFGE into 20 profiles with different clusters, while they were subtyped by 16S rRNA-RFLP into 9 profiles with a single cluster. Out of two Salmonella isolates, the invasion gene (invA) was detected in all other Salmonella isolates (94%) and the virulence gene (spvC) was detected in 11% of Salmonella isolates. Our results suggested that the PFGE subtyping is the prominent method for the evaluation and benchmarking of molecular subtyping.

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Detection of Non Typhoidal Salmonella Isolated from Food Products and Clinical Cases by PCR and Conventional Methods : a Comparative Study

Cited by 2 publications

References 13 publications

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Genetic diversity of food originated Salmonella isolates

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