Detection of Phenazines from UPMP3 Strain of Pseudomonas aeruginosa and its Antagonistic Effects against Ganoderma boninense

Abstract

To cite this paper: Parvin, W., R. AbstractTo identify the phenazine antibiotics from the selected UPMP3 strain of Pseudomonas aeruginosa and assessment of its antagonistic activity of Ganoderma boninense, three antibiotics phenazine (PHZ), phenazine-l-carboxylic acid (PCA) and pyocyanin (PYO) were extracted from the bacterial fermented broth using benzene and chloroform and detected through High-performance liquid chromatography (HPLC). The effects of three antibiotics on suppressing mycelial growth of G. boninense were assayed. The maximum amount of PHZ, PCA and PYO were recorded as 1.36, 9.62 and 15.48 µg/mL, respectively and showed in vitro antagonistic activity against G. boninense. Phenazine was found to be more effective than PCA and PYO to inhibit the mycelial growth of G. boninense. It was 100% for PHZ at 1.0 mg/mL, while 78.61% for PCA and 91.87% for PYO after 7 days of incubation. Phenazine, PCA and PYO were identified as first time from oil palm rhizospheric UPMP3 strain of P. aeruginosa. The results indicated that the identified PHZ, PCA and PYO compound had good antifungal activity against G. boninense, however the PHZ can be developed as an ideal biofungicide for the control basal stem rot disease of oil palm and helps to overcome the concerns about the residual effects of synthetic fungicides.

Phenazine from Pseudomonas aeruginosa UPMP3 induced the host resistance in oil palm (Elaeis guineensis Jacq.)-Ganoderma boninense pathosystem

“…On an individual evaluation, each antibiotic showed in vitro antagonistic activity against Ganoderma boninense at a minimal inhibition ratio of 1: 10: 15/PHZ: PCA: PYO. The PHZ extracted from P. aeruginosa UPMP3 crude extract demonstrated the best antifungal efficiency against Ganoderma boninense amongst the other phenazine derivatives evaluated at a laboratory scale experiment 12 .…”

Efficiency of Phenazine Compound Produced by Rhizospheric Pseudomonas fluorescens against Few Pathogenic Bacteria Isolated from Ear Infections

“…The RF value of 0.7 corresponds to the phenazine antibiotic in a 9:1 v/v solvent solution containing chloroform and methanol. The outcomes were discovered to be comparable to those discovered by (22) using TLC analysis to purify phenazine with RF 0.70.…”

“…21 The blue-green solution of P. aeruginosa in KA liquid medium and the crude extraction eluted with dichloromethane and methanol revealed yellow or yellow-green and blue hue indicating phenazine and pyocyanin synthesis. [22][23][24]…”

Biofungicide Producing Bacteria: an In Vitro Inhibitor of Ganoderma boninense

“…Both enzymes are enzymes that can degrade the cell wall of the fungus. The inhibitory capability of the bacteria is also reinforced by Parvin et al (2016) statement that P. aeruginosa isolated from the palm oil plant rhizosphere produces metabolite products such as phenazine in order to inhibit the growth of G. boninense.…”