Detection of potent insect antigens for humans: Hemoglobins (erythrocruorins) of chironomids

Baur, Xaver; Ziegler, David D.; Reichenbach-Klinke, H.; Aschauer, H; Braunitzer, Gerhard

doi:10.1007/bf01106601

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…The two cases of Chironomus thummi allergy were reported by Baur and co-workers (1980) 94. They reported that 20% of sera from 642 chironomids exposed subjects developed immediate-type hypersensitivity and had significantly raised levels of specific IgE antibodies.…”

Section: Clinical Studies With Different Insectsmentioning

confidence: 88%

A review on Respiratory allergy caused by insects

Kausar¹

2018

Bioinformation

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Hypersensitivity or allergy encompasses a wide range of immunological reactions that generally have adverse consequences involving one or many organ systems of the body. Allergens are usually glycoprotein or chemically complex low molecular weight substances. The common allergens include pollen, fungal spores, house dust mite and house dust, animal danders, drugs, foods, insect emanations, and detritus, etc. Information on the role of insects in respiratory allergy is increasing in the literature. There are about 30 million living species of insects. These insects can broadly be classified as stinging insects, biting insects and non-stinging and non-biting insects. All materials form insets namely wings, scales, saliva; dried feces and venom can cause allergic diseases, such as rhinitis, conjunctivitis, asthma and urticaria. There are wide varieties of insects such as moths, butterflies, bees, wasps, hornets, yellow jackets, flies, beetles, cockroaches, and mosquitoes. Exposure to emanations and detritus of these insects may lead to several allergies in some genetically predisposed individuals. Therefore, it is of interest to review allergies caused by various insect’s stings and bites and their adverse effect on the human body.

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Section: Clinical Studies With Different Insectsmentioning

confidence: 88%

A review on Respiratory allergy caused by insects

Kausar¹

2018

Bioinformation

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“…It was also shown that the Pol n I component MV binds to hu man IgE of asthmatic children. Furthermore, we demon strated that epitopes(s) for human IgE exist at N-terminal residues of the Pol n I component MV Hypersensitivity to chrionomids have been reported worldwide: in Europe [7], in Africa [2], in Asia [3,4] and in North America [11], Chironomid species of several distant ly related taxa have been shown to be potent sensitizing agents [12,13], However, ecological morphological and biochemical characteristics of allergenicaily important spe cies have been poorly understood. In this study, a species, PN, which is ubiquitous and emerges in large numbers from eutrophicated lentic waters in Japan [14], was for the first time demonstrated to be a possible causative agent of bron chial asthma.…”

Section: Purification and Isolation O F Allergenic Hh(s) From The Larmentioning

confidence: 99%

Purification and Characterization of an Allergenic Monomeric Hemoglobin from a Chironomid Distributed Worldwide, <i>Polypedium nubifer</i>

Kawai

Tagoh

Yoshizaki

et al. 1996

Int Arch Allergy Immunol

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The Pol n component MV, a potent experimental allergen for mice, was purified to homogeneity from extracts of a chironomid distributed worldwide, Polypedium nubifer (PN). The Pol n I component MV was shown to have cross-reactivity to hemoglobins (Hb) derived from all species of chironomids tested. Determination of the amino acid sequence of the first 37 N-terminal residues revealed that it had 30–59% homology to Hb of an European chironomid, Chironomus thummi thummi, which had been known as an important allergen for humans. By Western blot analysis, we showed that sera from asthmatic patients, which had positively reacted to the extract of the adult PN midge, bound to the purified Pol n I component MV Furthermore, using rabbit polyclonal antibodies raised against synthetic polypeptides corresponding to the N-terminal residues, it was demonstrated that the N-terminal amino acid sequence between position 15 and 35 contained antigenic epitope(s) for human IgE. The results indicate that the Pol n I component MV is an allergen for human beings as well as for mice, and useful as a diagnostic tool for chironomid allergy.

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“…A substantial part of the Sudanese population living along the Nile river are sensi tized and suffer from asthma and allergic rhinitis as a conse quence of the exposure to the environmental airborne aller gens after mass hatchings of the midges [1][2][3]. Atopic pa tients living around a reservoir who were sensitized with the lake fly, Chironomus plumosus (CP), were reported in Wis consin, USA [4], In Germany, hemoglobins (Hbs; Clii 11) of the larvae of Chironomus thummi thummi (CTT) were shown to be the major allergens among aquarists and workers in fish food factories [5][6][7], Railway workers allergic to Chi ronomus salinarius were also reported in Venice, Italy [8].…”

Section: Introductionmentioning

confidence: 99%

Occupational Allergy to Adult Chironomid Midges among Environmental Researchers

Teranishi

Kawai

Murakami³

et al. 1995

Int Arch Allergy Immunol

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A case of occupational allergy to chironomid midges in research work is described. A researcher was exposed to adult chironomid midges during his research and developed allergic rhinitis after 10 years of such exposure. Using the midge extract of adult Chironomus plumosus (CP) (Linnaeus, 1758), both immediate skin test and the ophthalmic challenge test gave positive results. IgE antibody against adult CP was also demonstrated by the radioallergosorbent test. Four of the five serum samples of the environmental researcher examined showed a positive radioallergosorbent test to at least one of the adult midges breeding around eutrophic Japanese lakes. Enzyme-linked immunosorbent assay inhibition test and immunoblot experiments indicated that the remaining hemoglobin is one of the major allergens of adult CP. These results demonstrate that the exposure to adult chironomid midges is an important occupational hazard among environmental researchers.

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Detection of potent insect antigens for humans: Hemoglobins (erythrocruorins) of chironomids

Cited by 9 publications

References 4 publications

A review on Respiratory allergy caused by insects

A review on Respiratory allergy caused by insects

Purification and Characterization of an Allergenic Monomeric Hemoglobin from a Chironomid Distributed Worldwide, <i>Polypedium nubifer</i>

Occupational Allergy to Adult Chironomid Midges among Environmental Researchers

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