Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR

Anderson, Burt; Sims, Kimetha G.; Regnery, Russell L.; Robinson, Laura; Schmidt, Martin J.; Goral, Simin; Hager, Cynthia; Edwards, Kathryn M.

doi:10.1128/jcm.32.4.942-948.1994

Cited by 263 publications

(91 citation statements)

References 27 publications

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“…Most CSD cases in the past have been diagnosed by clinical and pathologic criteria, confirmed in later studies by a nonstandardized skin test (14). It is only since the identification of B henselae as the etiologic agent of CSD in the early 1990s that more sensitive, specific, and readily available diagnostic tests, particularly serology (9, 18) and PCR (11, 19), have been introduced, allowing for the association of new clinical syndromes, such as arthropathy, with CSD.…”

Section: Discussionmentioning

confidence: 99%

Cat‐scratch disease–associated arthropathy

Giladi¹,

Maman²,

Paran³

et al. 2005

Arthritis & Rheumatism

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Objective. To characterize the articular manifestations of cat-scratch disease (CSD) and to evaluate the long-term clinical outcome of those manifestations.Methods. A community-and hospital-based surveillance study of CSD was conducted in Israel between 1991 and 2002. CSD was defined as present in a patient when a compatible clinical syndrome and a positive confirmatory finding of Bartonella henselae (by serology and/or polymerase chain reaction) were identified. CSD patients with arthropathy (arthritis/arthralgia) that limited or precluded usual activities of daily living constituted the study group. Patients were followed up until >6 weeks after resolution of symptoms, or if symptoms persisted, for >12 months. CSD patients without arthropathy served as controls.Results. Among 841 CSD patients, 24 (2.9%) had rheumatoid factor-negative arthropathy that was often severe and disabling. Both univariate and multivariate analyses identified female sex (67% of arthropathy patients versus 40% of controls; relative risk [RR] 2.5, P ‫؍‬ 0.047), age older than 20 years (100% of arthropathy patients versus 43% of controls; RR 4.9, P ‫؍‬ 0.001), and erythema nodosum (21% of arthropathy patients versus 2% of controls; RR 7.9, P ‫؍‬ 0.001) as variables significantly associated with arthropathy. Knee, wrist, ankle, and elbow joints were most frequently affected. Ten patients (42%) had severe arthropathy in the weight-bearing joints, which substantially limited their ability to walk, and 4 of these patients were hospitalized. All of the patients had regional lymphadenopathy, 37.5% had nocturnal joint pain, and 25% had morning stiffness. Nineteen patients (79.2%) recovered after a median duration of 6 weeks (range 1-24 weeks), whereas 5 patients (20.8%) developed chronic disease persisting 16-53 months (median 30 months) after the onset of arthropathy.Conclusion. This is the first comprehensive study of arthropathy in CSD. CSD-associated arthropathy is an uncommon syndrome affecting mostly young and middle-age women. It is often severe and disabling, and may take a chronic course.Cat-scratch disease (CSD) is a common zoonosis caused principally by Bartonella henselae. Typical CSD, which occurs in ϳ90% of CSD cases, is characterized by subacute regional lymphadenitis that is often associated with systemic symptoms such as fever and malaise. When CSD affects other organs, as occurs in ϳ10% of patients, the disease is designated atypical CSD. Atypical CSD includes Parinaud's oculoglandular syndrome, neuroretinitis, encephalitis, peripheral neuritis, granulomatous hepatitis, and/or splenitis, osteomyelitis, erythema nodosum, pneumonitis, and prolonged fever of unknown origin (1). Over the last decade we have occasionally observed CSD patients with severe, protracted, and often disabling arthropathy. A literature search identified only a few CSD patients with arthropathy having been reported since 1950 (2-7), when "la maladie des griffes de chat" was first defined as a syndrome (8), raising doubts regarding whether arthropathy is a r...

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Section: Discussionmentioning

confidence: 99%

Cat‐scratch disease–associated arthropathy

Giladi¹,

Maman²,

Paran³

et al. 2005

Arthritis & Rheumatism

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“…Isolation of B. henselae from patients is extremely difficult (La Scola & Raoult, 1999). The diagnosis of CSD relies on clinical manifestations, history of contact with cats, serology, or the detection of bacterial DNA in tissue specimens by PCR (Regnery et al, 1992;Anderson et al, 1994;Murakami et al, 2002;Woestyn et al, 2004;Tsuneoka & Tsukahara, 2006). Bartonella henselae strains are divided into two 16S rRNA (rrs) genotypes (16S type I/Houston-1 and 16S type II/Marseille), which correspond to two distinct human serotypes (Drancourt et al, 1996;La Scola et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan

Yanagihara

Tsuneoka

Hoshide

et al. 2010

FEMS Immunology &Amp; Medical Microbiology

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Bartonella henselae is the causative agent of cat scratch disease (CSD). To clarify the population structure and relationship between human and cat strains of B. henselae, 55 specimens isolated in Japan, including 24 B. henselae DNA-positive clinical samples from CSD patients and 31 B. henselae isolates from domestic cats, were characterized by multilocus sequence typing (MLST) and the 16S-23S tRNA-Ala/tRNA-Ile intergenic spacer (S1) sequence, which were used previously for strain typing of B. henselae. Three different sequence types (STs) were identified by MLST, one of which was novel. Fifty-two strains (94.5%), including all strains detected in CSD patients, were assigned to ST-1. Eight S1 genotypes were observed, three of which were novel. The 52 ST-1 strains were classified into seven S1 genotypes, two of which were predominant in both human and cat strains. In addition, 5.5% of the strains (3/55) contained two different intergenic spacer S1 copies. These results indicate that the predominant B. henselae MLST ST-1 in Japan is a significantly genetically diverse population on the basis of the sequence diversity of intergenic spacer S1, and that highly prevalent S1 genotypes among cats are often involved in human infections.

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“…In 1931 Debré observed the occurrence of regional lymphadenopathy following cat scratches, then 20 years later published a report of "la maladie des griffe du chat", establishing CSD as a clinical entity (1)(2)(3). Serological testing for this organism in humans and their cats, as well as the culture of B henselae and the detection of B henselae DNA from lymph nodes in patients with clinical disease, supports the role of this organism in CSD (4)(5)(6)(7). Formerly known as Rochalimaea henselae, the organism was reclassified as B henselae in 1993, following 16S rRNA sequence analysis (8).…”

mentioning

confidence: 88%

“…The IFA test currently available has shown significant crossreactivity between B henselae and B quintana (79). While BA, BP and trench fever are caused by B quintana, this organism has not been identified in specimens from patients with CSD by culture or PCR (6,48,79). Interpretation of a positive IFA test for Bartonella species should therefore be made in the context of clinical presentation.…”

Section: Diagnosis Of Csdmentioning

confidence: 99%

“…Although not available as a routine diagnostic test, PCR hybridization assays that detect and differentiate between B henselae and B quintana directly in clinical specimens have been developed (48,83). Anderson et al (6) developed a PCR dot blot assay using primers that amplify a 414 base pair fragment of DNA common to B henselae and B quintana; internal oligonucleotides were then used as hybridization probes to differentiate between the Bartonella species. Using PCR hybridization for bartonella DNA of 89 lymph node aspirates from CSD skin test-positive patients and 137 aspirates and biopsies from CSD suspects, 96% and 60% were positive, respectively (7).…”

Section: Diagnosis Of Csdmentioning

confidence: 99%

See 1 more Smart Citation

Cat Scratch Disease: The Story Continues

Opavsky

1997

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Evidence accumulated to date supports B henselae as the etiological agent of CSD. The most significant risk factors for CSD are being licked on the face, scratched or bitten by a kitten and owning a kitten with fleas. Available serological tests can confirm classic CSD and identify B henselae as the cause of more atypical presentations, such as fever of unknown origin, granulomatous hepatitis, encephalitis and osteomyelitis. Symptomatic management is appropriate for isolated lymphadenopathy caused by CSD in healthy individuals; however, antibiotic therapy may be indicated for patients with more severe manifestations of the disease and immunocompromised hosts. Further study of CSD, in particular the epidemiology and therapy, is warranted. A better understanding of the pathogenesis of B henselae infection will have important implications in both immunocompetent and immunocompromised individuals.

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Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR

Cited by 263 publications

References 27 publications

Cat‐scratch disease–associated arthropathy

Cat‐scratch disease–associated arthropathy

Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan

Cat Scratch Disease: The Story Continues

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