Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe

Le, Hoan T.; Armstrong, K. C.; Miki, Brian

doi:10.1007/bf02669631

Cited by 144 publications

(64 citation statements)

References 14 publications

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“…1) shows that the lines analysed arose from two distinct crosses between wheat and rye (Zeller, 1973 and G. Zimmermann, personal communication). The variety Kavkaz (analysed by Le et aL, 1989) probably includes the same rye segment as Riebesel 47/51 (see fig. 1; Zeller, 1973;Zeller and Sastrosumarjo, 1972).…”

Section: Genomic Probing Of Southern Transfersmentioning

confidence: 99%

“…1; Zeller, 1973;Zeller and Sastrosumarjo, 1972). In all the wheat varieties carrying a 1B/1R translocation which have been examined, the translocation is at, or very close to, the centromere of the translocated chromosome (figs 4 and 5; Le et a!., 1989;Lapitan et a!., 1986, in variety Bobwhite). Thus, there is apparently no variation in the size of the translocated arm which would give a different combination of wheat and rye genes in the variety.…”

Section: Genomic Probing Of Southern Transfersmentioning

confidence: 99%

“…Hybridization with total genomic DNA can be used to distinguish chromosomes and chromosome segments originating from different genomes in barley x rye species hybrids (Heslop-Harrison et a!., 1988;Schwarzacher et al, 1989) and in triticale (wheatx rye) and 1B/1R wheats (Le et al, 1989). The genomic probing method potentially allows accurate determination of chromosome breakpoints.…”

Section: Introductionmentioning

confidence: 99%

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Detection and characterization of 1B/1R translocations in hexaploid wheat

et al. 1990

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Total genomic DNA from rye was labelled with biotin and used as a probe for in situ hybridization to show the sizes and translocation points of the rye chromosome segments in five wheat varieties which carry a translocation between wheat chromosome lB and the short arm of rye chromosome 1R (1B/IR). All the translocation breakpoints were at, or very near to, the centromere. Using genomic DNA to block some cross-hybridization, little signal from hybridization between the rye probe and wheat chromosomes was observed despite the 78 per cent sequence homology between the species. The translocation was identified in cells at all stages of the cell cycle. Total genomic rye DNA used as a probe was also able to distinguish rye, triticale and wheat varieties carrying the translocation, by Southern hybridization. The techniques using genomic probes are useful for detecting, characterizing and following alien chromosomes or chromosome segments through breeding programmes, by both in situ and Southern hybridization.

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Section: Genomic Probing Of Southern Transfersmentioning

confidence: 99%

Section: Genomic Probing Of Southern Transfersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection and characterization of 1B/1R translocations in hexaploid wheat

et al. 1990

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“…Application of the fluorescent in situ hybridization (FISH) technique has become very useful in plants for: physical mapping of the DNA sequences due to the possibility of simultaneous detection of several probes on the same metaphase chromosome spread, nuclei or DNA fibers (Mukai et al 1993); identification and characterization of chromosomes or chromosome segments; providing indicators of recent or evolutionary rearrangements; understanding the phylogenetic and relationships among species (Jiang and Gill 1994); detection of single low copy or unique DNA sequences in plant chromosomes (Fransz et al 1996, Peterson et al 1999. It would enable molecular biologists, cytogeneticists, and breeders to locate and track alien gene and gene complexes that have been inserted into host plants by transformation (Le et al 1989, Salvo-Garrido et al 2001.…”

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confidence: 99%

rDNA Loci and DAPI Bands Reflect the Phylogenetic Distance between Vicia Species

Ali¹,

El-Khodary²,

El-Ashry³

et al. 2009

CYTOLOGIA

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SummaryThe chromosomal positions of 5S and 25S rRNA genes, as well as of DAPI (4Ј,6-diamidino-2-phenylindole) bands are described for 5 Vicia species (Vicia macrocarpa, Vicia sativa, Vicia narbonensis, Vicia ervilia and Vicia faba) to find out the phylogenetic relationships among studied species. From the results, it was concluded that the phylogenetic relationships among the studied species are as follows: Vicia ervilia is closely related to Vicia narbonensis and Vicia narbonensis is related to Vicia macrocarpa. However, the degree of relation between Vicia narbonensis, and Vicia macrocarpa is less than the relation between Vicia narbonensis and Vicia ervilia, while Vicia sativa is closely related to Vicia macrocarpa, but Vicia faba is far from all studied species.

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“…A complete understanding of the genomic constitution of specific wheat germplasm is useful not only for genetic studies, but also for the selection of parents in breeding programs. Methods based on cytological and molecular techniques such as C-banding (Gill et al 1991) and genomic in situ hybridization (GISH) (Le et al 1989;Schwarzacher et al 1989;Islam-Faridi and MujeebKazi 1995;Ahmad et al 2000) provide powerful tools to identify alien chromosomes or chromosome segments that have been integrated into the wheat genomes. For GISH analysis, total genomic DNA from the alien species is usually used as a probe to detect the alien chromosomes introgressed into wheat.…”

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confidence: 99%

Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition

Li¹,

Conner²,

McCallum³

et al. 2004

Can. J. Plant Sci.

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The hard red winter wheat Tangmai 4 did not develop symptoms of infection following inoculation with powdery mildew (Erysiphe graminis DC. f. sp. tritici E. Marchal) isolates from regions of western Canada and northern China. Tangmai 4 exhibited resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. & Henn.) and leaf rust (P. triticina Eriks.) races from western Canada. This wheat line was resistant to individual stripe rust (P. striiformis Westend. f. sp. tritici Eriks.) races from the U.S. and Canada. Sequential C-banding and genomic in situ hybridization (GISH), and electrophoretic analyses of high molecular weight glutenins and gliadins demonstrated that Tangmai 4 carried a pair of T1BL·1RS wheat-rye (Secale cereale L.) translocated chromosomes. Since the genes located on T1BL·1RS are no longer effective in controlling powdery mildew and the rust diseases, Tangmai 4 must carry additional genes for resistance to these diseases, which makes it a valuable resource for the improvement of resistance in wheat against these diseases. Key words: T1BL·1RS translocation, disease resistance, sequential C-banding and GISH, glutenin, gliadin

show abstract

Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe

Cited by 144 publications

References 14 publications

Detection and characterization of 1B/1R translocations in hexaploid wheat

Detection and characterization of 1B/1R translocations in hexaploid wheat

rDNA Loci and DAPI Bands Reflect the Phylogenetic Distance between Vicia Species

Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition

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