Detection of Transposon Gene in Lurik Peanuts (Arachis hypogaea var. lurik L.) with AhMITEs Analysis

Abstract

Peanut (Arachis hypogaea L.) is one of the leading commodities in Indonesia that are consistently growing with high demand. However, its productivity in the current state is relatively few, thus causing dependency on imported products. Developing new varieties is one of the many solutions to these problems. Lurik peanuts are superior local varieties to any other peanuts in terms of productivity and disease resistance. Seed with the purple pattern is this cultivar's special characteristic and main attraction. This study aimed to identify and verify the activity of transposon genes in the seed pattern of Lurik peanuts. This research method was carried out by gene detection and sequencing analysis using PCR-AhMITEs (Arachis hypogaea Miniature Inverted Transposable Elements). The study used the Garuda variety as a comparison due to the absence of seed patterns, and it is a superior variety widely cultivated in Indonesia. Four types of primers used in this study were AhMITE1, AhTE0357, AhTE0391, and AhTE1317. The results revealed that the four primers had a linear relationship that could distinguish Lurik peanuts and Garuda peanuts based on the presence of transposon genes. The sequencing results confirmed that the detected genes were transposons from peanuts, located on chromosome 5 (Arahy.5), chromosome 9 (Arahy.9), chromosome 14 (Arahy.14), and chromosome 19 (Arahy.19). Based on the results of the study, the pattern on Lurik peanuts is an expression of the transposon gene activity.