Detection Rifampicin Resistance Tuberculosis Using Xpert MTB/RIF Assay in Saiful Anwar Hospital Malang

Abstract

Tuberculosis is still problem in Indonesia. The increase number tuberculosis patientsand then multidrug resistance tuberculosis the higher it. Xpert MTB /RIF assay ,an automated real time polymerase chain reaction assay, detects the presence Mycobacterium tuberculosis and rifampicin resistance to rifampicin in sputum within 2 hours. Here we report the epidemiology Mycobacterium tuberculosis of RIF -resistant TB pattern in Saiful Anwar Hospital Malang city. Material methods: A total of 3063 sputum specimen received in microbiology laboratory Saiful Anwar Hospital Malang between Januari 2013 until Desember 2016 were tested by Xpert MTB/RIF assay following manufactur's instruction.. Sputum specimen from patients suspect TB-MDR and TB-HIV. Result : Total of 645 sputum specimen from TB-HIV patients suspect were detected rifampicin resistance 0,3% and .21,1% rifampicin sensitive. The Total 2418 .from TB-MDR patients suspect were detected 8.1 % rifampicin resistance and 46.6.% rifampicin sensitive. Conclusions : Xpert MTB/RIF assay can use screening tool for detection of Mycobacterium tuberculosis and Rifampicin resistance from suspected TB-MDR and TB-HIV cases in a shorter periode of time.