Detection, separation and analysis of α-amino acids by means of TLC using 4-diethylaminodiazabenzene-4′-isothiocyanate (DEABITC)

Wawrzycki, S.; Pyra, E.

doi:10.1007/bf02492824

Cited by 7 publications

(2 citation statements)

References 7 publications

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“…Wawrzycki and Pyra () reported that derivatization of α ‐amino acids using 4‐(diethylamino)azobenzene‐4–‐phenyl isothiocyanate with DEABITC‐amino acids improves separation and facilitates detection of selected protein amino acids (in picomoles).…”

Section: Thin‐layer Chromatographymentioning

confidence: 99%

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Dołowy

Pyka

2013

Biomedical Chromatography

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Amino acids are very important organic compounds in nature. The biological activity of amino acids depends mainly on their stereoisomeric configuration (d- or l-). Thus, the stereochemical analysis of amino acids and peptides is an important aspect of their characterization. Owing to the increasing role of amino acid configuration in biomedical and pharmaceutical studies, numerous analytical methods have been described in the literature. Among a wide range of analytical techniques available for the steroselective separation of different amino acids, which were obtained from plants or biological samples, chromatographic methods such as thin-layer chromatography, high-performance chromatography and also gas chromatography are very useful. This review presents such systems developed for direct stereoisomeric separation and quantitative determination of amino acid and peptide enantiomers with emphasis on selected literature published during two last decades. Almost all aspects, including sample preparation prior chromatographic analysis, stationary phase, solvent system and detection system, are discussed. New possibilities in chiral amino acid analysis have been opened up by the application of mass spectrometry and infrared detection in thin-layer chromatography and mass spectrometry-selected ion monitoring detection system in gas chromatography. A modern trend in high-performance chromatography is two-dimensional chromatography. These innovations have led to decreased development time and increased amino acid resolution and detectability.

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Section: Thin‐layer Chromatographymentioning

confidence: 99%

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Dołowy

Pyka

2013

Biomedical Chromatography

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“…The following are examples of analyses that include the formation of derivatives prior to TLC: the formation of fluorescent dansyl derivatives for determination of biogenic amines in red wine (79) and other foods (80) and of colored thiocarbamoyl derivatives of biogenic amines (81); the use of /?-benzoquinone for derivatization of 2-(methylamino)ethanol and other primary and secondary amines (82); the separation of /?-dimethylaminobenzaldehyde from p-dimethylaminocinnamaldehyde after derivatization with diphenylamine (83); determination of bisoprolol, labetalol, and propafenone as dabsyl derivatives in pharmaceutical preparations (84); and determination of the toxin fumonisin B-l in corn after immunoaffinity column cleanup and derivatization (85). In many cases, enantiomers have been resolved by TLC after the formation of derivatives, e.g., amino acids derivatized with l-fluoro-2,4-dinitrophenyl-5-L-alamne amide, and separated on RP plates (86).…”

Section: Derivatizationmentioning

confidence: 99%

Basic TLC Techniques, Materials, and Apparatus

Sherma¹

2003

Handbook of Thin-Layer Chromatography

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Chromatography of amino acids and short peptides. New advances

Cserháti

2007

Biomedical Chromatography

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The newest results in the application of various chromatographic methods (gas-liquid chromatography, liquid chromatographic techniques, electrically driven systems) for the separation and quantitative determination of amino acids and short peptides in pure state and in complicated matrices are compiled. The results are concisely described and critically evaluated. The future trends of the chromatographic analysis of amino acids and short peptides are briefly discussed.

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Detection, separation and analysis of α-amino acids by means of TLC using 4-diethylaminodiazabenzene-4′-isothiocyanate (DEABITC)

Abstract: Summary4-diethylaminodiazabenzene-4'-isothiocyanate (DEA-BITC) was synthetized and used to obtain thiohydantoin derivatives of 15 c~-amino acids (DEABTH-AMK). The coloured derivatives were separated by means of TLC on silica gel using various solvent systems.

Cited by 7 publications

References 7 publications

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Basic TLC Techniques, Materials, and Apparatus

Chromatography of amino acids and short peptides. New advances

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