DETERMINACION DEL EFECTO DE LA CONCENTRACION DE CELULASA, CELOBIASA Y NaOH EN LA HIDRÓLISIS PARA LA PRODUCCION DE ETANOL A PARTIR DEL RASTROJO DE LA PIÑA

Abstract

ResumenEl objetivo de esta investigación fue determinar el efecto de la concentración de la celulasa, celobiasa y del hidróxido de sodio en la hidrólisis de la celulosa del rastrojo de piña. El rastrojo de la piña de la variedad MD2 utilizado contiene: 86,4% de humedad, 12,06% de cenizas en base seca, 66,2% de holocelulosa y 8,3% lignina en base seca libre de extractos. Se realizó el pretratamiento a diferentes concentraciones de NaOH y diferentes tiempos encontrándose que el rendimiento más alto corresponde a un tiempo de 25 minutos. Para muestras pretratadas con NaOH al 1,00% y 1,25% (m/v) a una temperatura de 121 °C por 25 minutos, concentraciones de celulasa de 10, 15 y 20 FPU/g; una relación celulasa:celobiasa (v:v) de 5:1, 7:1 y 9:1 respectivamente, estadísticamente no se encontró diferencia significativa entre la concentración de hidróxido de sodio y la relación de enzima celulasa:celobiasa en el rendimiento de glucosa; por lo que se establece una concentración de NaOH de 1,00% en la etapa de pretratamiento y una relación de enzimas de 7:1. En la sacarificación y fermentación simultáneas utilizando cantidades de rastrojo de (25,10; 35,10 y 50,30) g/L se determinó que los rendimientos fueron de 56,26%; 52,45% y 50,33% respectivamente; resultados obtenidos al cabo de 25 horas de hidrólisis. La concentración de etanol promedio obtenida es de 1,87% v/v.Palabras clave: rastrojo de piña; material lignocelulósico; pretratamiento alcalino; etanol celulósico. AbstractThe main goal of this research was to determine the effect of cellulase, celobiase and sodium hydroxide concentration in the hydrolysis of cellulose of pineapple's straw. The straw used was obtained from pineapple variety MD2 and had this composition: 86,4% of water, 12,06% of ash (d.b), 66,2% of holocellulose and 8,2% of lignin (d.b free extracts).A pretreatment was executed at different times and concentrations of NaOH, and it was found that the highest yield is obtained at 25 minutes.For pretreated samples at test conditions of NaOH at 1,00% and 1,25%(m/v), 121 °C for 25 minutes, a cellulase concentration of 10, 15 and 20 FPU/g and cellulase: celobiase ratio (v:v) of 5:1, 7:1 and 9:1, it was found that there is no statistical difference between NaOH concentration and cellulase: celobiase ratio in the yield for obtaining glucose. It was fixed NaOH concentration of 1,00% for the pretreatment stage and enzymes ratio of 7:1.In the simultaneous saccharification and fermentation for different concentrations of straw, it was determined that for 25 hours of hydrolysis, the yields for obtaining glucose were 56,26% for a straw concentration of 25,10 g/L, 52,45% for a concentration of 35,10 g/L and 50,33% for a concentration of 50,30 g/L. The mean ethanol concentration obtained was 1,87% v/v.

Pre-treatments applied to rice husk enzymatic hydrolysis: effect on structure, lignocellulosic components, and glucose production kinetics

“…The morphological changes of the rice husk by applying the different pre-treatments were evidenced in Figure 1. The structure of the rice husk without any pre-treatment (Figure 1, Control) shows the rigid and orderly epidermal tissue due to the presence of amorphous hemicelluloses in its area [13], also had none pores showing the appearance of interrupted cells. It is due to the crossing of their components [22].…”

“…Using rice husk, it was studied freezing pre-treatment [9] and alkaline pre-treatment [10], pre-treatments with laccase from Lentinus polychrous [11], in all cases the enzymatic digestibility was increased. On the other hand, mechanical fractionation pre-treatments at different levels were applied in corn cobs [12]; cellobiose, cellulase and NaOH in pineapple stubble [13]; H2O2, H2SO4 and NaOH in cassava residues [14]; steam explosion, acid and alkaline pre-treatment in pine sawdust [15].…”

Biocomposite Films of Polylactic Acid Reinforced with Microcrystalline Cellulose from Pineapple Leaf Fibers

“…1 shows the chemical composition of PALF obtained in this work in comparison to those reported in earlier works. Values reported by Quesada [8] and Cordoba [24] were both obtained with local PALF samples according to standard ASTM methods, whereas results reported by Mukherjee and Satyanarayana [25], Mohanty et al [26], and Mishra et al [27] were obtained with Indian PALF substrates and no details on test procedures were declared. In addition, values reported by Khalil et al [28] were obtained with Malaysian PALF substrates using TAPPI methods.…”

Production of renewable fuel and value-added bioproducts using pineapple leaves in Costa Rica

“…[15,16]. Different pretreatment methods have been used to improve ethanol production [17,18]. The use of pineapple leaf in combination with molasses for ethanol production was investigated by integrating steam explosion pretreatment and separated hydrolysis and fermentation (SHF) [19].…”