Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis

Mishra, Vineet Kumar; Passari, Ajit Kumar; Chandra, Preeti; Leo, Vincent Vineeth; Kumar, Brijesh; Uthandi, Sivakumar; Binodh, Asish K.; Gupta, Vijai Kumar; Singh, Bhim Pratap

doi:10.1371/journal.pone.0186234

Cited by 82 publications

(34 citation statements)

References 83 publications

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“…Tetracycline was used as a positive control, with IZDs ranging from 13 to 21 mm (Akinyemi 2017). Mishra et al (2017) observed that ethyl acetate extract of the endophytic fungus A. clavatonanicus (MJ31), isolated from roots of Mirabilis jalapa (Nyctaginaceae), displayed antibacterial potential against B. subtilis, M. luteus, and S. aureus, with MICs of 0.078, 0.156, and 0.312 mg ml −1 , respectively.…”

Section: Antibacterial Properties Of Crude Extractsmentioning

confidence: 99%

Overview on antibacterial metabolites from terrestrialAspergillusspp

Al-Fakih

Almaqtri

2019

Mycology

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Medicines developed from natural sources are a frequent target for the research and discovery of antimicrobial compounds. Discovering of penicillin in 1928 was a motive to explore of nature as a source of new antimicrobial agents. Fungi produce a diverse range of bioactive metabolites, making them rich source of different types of medicines. The purpose of this paper was to review studies on antibacterials from terrestrial Aspergillus published exclusively during 1942-2018, with emphasis on their antibacterial activities, structures, and mechanisms of action if present. According to the results from different studies in the world, large number of compounds and extracts showed different activities against different bacterial species, including Gram-positive and Gram-negative bacteria. The most prominent result was that of the compound CJ-17,665, isolated from A. ochraceus, showing good activity against multi-drug resistant Staphylococcus aureus, which is well-recognised to be one of the most important current public health problem. These findings may motivate scientists to undertake a project that may result in the development of novel antibacterial drugs from terrestrial-derived Aspergillus spp., although further toxicity assays (in vivo) must be performed before their application.

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Section: Antibacterial Properties Of Crude Extractsmentioning

confidence: 99%

Overview on antibacterial metabolites from terrestrialAspergillusspp

Al-Fakih

Almaqtri

2019

Mycology

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“…They can synthesise multiple metabolites, which allow them to adapt to the variety of environments they inhabit. Some of these primary and secondary metabolites have been commercially exploited; generating several Aspergillus -related products and patents including antibiotics, anti-tumoral and anti-fungal agents as well as other compounds with a medical application, such as the cholesterol-lowering drug lovastatin [5,6,7,8,9]. Additionally, Aspergilli strains are involved in many modern industrial processes including the production of hydrolytic enzymes (e.g., pectinases) [10] and bulk chemicals (e.g., citric acid) but have also been used for centuries in Asia for the production of fermented foods, such as sake, soy sauce and miso [11].…”

Section: Introductionmentioning

confidence: 99%

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

et al. 2019

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This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.

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“…Primary ITS-4 and ITS-5 were used in PCR amplification (Liang et al 2012;Pansanit and Pripdeevech 2018). The PCR amplicon was sequenced and blasted by using National Center for Biotechnology Information (NCBI) (Mishra et al 2017). Fungal DNA with the highest similarity selected and grouped using ClustalW program (Hannula and Veen 2016) in txt, MSF, and PHYLIP formats (Felsenstein 1989).…”

Section: Identification Of Endophytic Fungimentioning

confidence: 99%

Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks

et al. 2019

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Abstract. Fadhillah, Elfita, Muharni, Yohandini H, Widjajanti. 2019. Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks. Biodiversitas 20: 2645-2650. Swietenia mahagoni L. Jacq is a medicinal plant that widely used as an antimicrobial, anti-inflammatory, antimutagenic, anticancer, antitumor, and antidiabetic. Several bioactive compounds have been reported to be associated with this plant that supports its treatment activities. Flavonoid content of the S. mahagoni has antioxidant activity. Therefore the plant is widely used to treat degenerative diseases caused by free radical activity in the body. Scientists have proven that the medicinal plants have a symbiotic relationship with the number of microorganisms that can produce bioactive compounds. One of the symbiotic microorganisms is endophytic fungi believed to be associated with every plant on earth. In this paper, we report a secondary metabolite compound from endophytic fungi isolated from S. mahagoni. The endophytic fungi (DMG1-DMG4) were isolated from leaf stalks of S. mahagoni. Antioxidant activity of endophytic fungi extracts was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The chemical compound from the active extract was isolated by the chromatographic method, and the structure was elucidated by spectroscopic methods including UV, IR, NMR 1D (1H-NMR and 13C-NMR), NMR 2D (HMQC and HMBC). Antioxidant activity test showed the endophytic fungus (DMG3) has the highest activity. The chemical compound from DMG3 was identified as 5-hydroxy-2-oxo-2H-piran-4-yl) methyl acetate). The phylogenetic method used to molecular analysis of DMG3 confirmed the fungus as Cladosporium tenuissimum.

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Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis

Cited by 82 publications

References 83 publications

Overview on antibacterial metabolites from terrestrialAspergillusspp

Overview on antibacterial metabolites from terrestrialAspergillusspp

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks

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