Determination colchicine content in aquadest-extracted Gloriosa superba seed from Sukoharjo and Gunung Kidul

Abstract

View the article online for updates and enhancements. Abstract. Colchicine is a toxic alkaloid compound from the Colchicum autumnale plant. Colchicine can be used in agriculture as anti-mitosis in the procurement of plants with polyploid cells. Gloriosa superba is an herb contains colchicine as a mutagen (polyploid) potential in its parts, especially in the seeds. The aims of this research were to determine colchicine content from aquadest-extracted Gloriosa superba seed obtained from Sukoharjo and Gunung Kidul. Gloriosa superba seed samples were obtained naturally in January -April 2017. Samples were divided into immature seed samples from Mulur, Sukoharjo, Central Java and old seed samples from Krakal Beach, Gunung Kidul, Yogyakarta. Extraction seeds of Gloriosa superba using the maceration method with aquadest solvent (1: 1). Determination content of colchicine extract using TLC-Densitometry method. The results indicated that colchicine content of old seed Gloriosa superba samples 37,6 µg/µl (± 6,63) higher than colchicine content of immature seed Gloriosa superba samples 12,84 µg/µl (± 2,88)

Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Colchicum autumnale Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters

“…For its determination in serum and plasma samples, various liquid chromatography-mass spectrometry (LC-MS) methods have been reported [1,[22][23][24]. For the determination of this compound in various commercial formulations and plant extracts, distinct high-performance thin-layer chromatography (HPTLC) assays have been reported [10,[25][26][27][28][29][30][31][32][33][34][35]. Some electrochemical techniques have also been utilized for the determination of this compound [36,37].…”