Détermination de la fraction du génome codant pour les RNA ribosomiques et messagers dans le cerveau du rat adulte

Stévenin, James; Samec, J.; Jacob, Monique; Mandel, P.

doi:10.1016/0022-2836(68)90319-7

Cited by 37 publications

(1 citation statement)

References 9 publications

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“…For example, Miyagi, Kohl, and Flickinger (1967) used hybridization techniques successfully in differentiating RNA of liver and kidney and RNA of embryo and adult chickens. Stevenin, Samec, Jacob, and Mandel (1968) reported the approximate amounts of the fraction of the genome in rat brain which codes for ribosomal and messenger RNAs. Likewise, Bondy and Roberts (1968) found these procedures essential in their finding that a much greater proportion of RNA synthesis is oriented toward messenger RNA formation in brain than in other organs.…”

Section: Methodological Problemsmentioning

confidence: 99%

Quantitative versus qualitative RNA and protein changes in the brain during behavior.

Gaito¹,

Bonnet²

1971

Psychological Bulletin

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Research purporting to demonstrate the occurrence of quantitative or qualitative changes in ribonucleic acid (RNA) and/or protein during simple and complex behavior is reviewed. Limitations in interpretation of research results and methodological problems are discussed. Much research indicates that quantitative changes do occur. In general, increments tend to result with mild or moderate stimulation; decrements, with drastic or prolonged stimulation. However, there is no conclusive evidence to indicate that qualitatively different RNA and/or protein species are synthesized during behavior. The necessity for research involving methods sensitive to the detection of qualitative changes is suggested.

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Section: Methodological Problemsmentioning

confidence: 99%

Quantitative versus qualitative RNA and protein changes in the brain during behavior.

Gaito¹,

Bonnet²

1971

Psychological Bulletin

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Messenger RNA in avian erythroblasts at the transcriptional and translational levels and the problem of regulation in animal cells

1968

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The RNA metabolism in immature duck erythrocytes has been investigated in order to determine the characteristics of messenger RNA (mRNA) in a highly differentiated animal cell. mRNA-like fractions were obtained from polysomes, on the one hand, and from pulse-labeled total cells or isolated nuclei, on the other, and were characterized by sedimentation, labeling kinetics, base composition, and hybridization to homologous DNA.At the translational level, the pulse-labeled RNA from polysomes consists of a predominant species sedimenting with about 9s and of a class of polydisperse material sedimenting between 6 and 28s. Very little ribosomal RNA (rRNA) is synthesized. The 9s RNA has been purified. Its base composition is relatively high in G + C (but different from rRNA or transfer RNA) -as determined, after alkaline hydrolysis, by azP distribution or spectrophotometric analysis. The polydisperse RNA has a base composition characterized by relatively high proportions of U and A and is similar in this respect to nuclear RNA. Total polysomal RNA hybridizes to homologous DNA. The biological activity tested in a cell-free protein-synthesizing system of Escherichia coli is highest in the 16 to 18s zone of polysomal RNA.The rapidly labeled RNA synthesized at the transcriptional level in the nuclei sediments predominantly in the 30 to 80s zone. Base-composition analysis of this RNA reveals the presence of a predominant fraction of high-U-type RNA and of a small amount of 45 and 32s rRNA precursors. The former fraction -tentatively termed nascent, messenger-like RNA (nascent mlRNA), with respect to its base composition and capacity of selective hybridization -is metabolically more unstable than the precursor rRNA.Hybridization experiments demonstrate that u p to 7% of the DNA is homologous to the nascent RNA fractions. Polysomal RNA hybridizes to a much smaller extent and competes only slightly with the heavy nuclear fractions.The significance of this heavy, nascent mlRNA and its eventual role in the regulation of protein synthesis in animal cells is discussed.We conclude that in a. highly differentiated cell many more mRNA species are produced than would be expressed phenotypically through protein synthesis in the polysome. A surprisingly large part of the genome is activated, but an important fraction of the transcription products never reaches the sites of protein synthesis. Thus, the spectrum of functional RNA is not defined through synthesis only, but is restricted during metabolism. Under these conditions, control of differentiation is probably not limited exclusively to the transcription of the genome, but is subject to regulation mechanisms operating at the intermediate or translational level. This paper shall give information on the pattern of RNA in a highly differentiated animal cell, the avian erythroblast. We would like to discuss our results and other published information on the mechanisms of regulation governing the information transfer from the genome to phenotypic expression in animal cells. We also ...

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RNA Metabolism

Mandel

Jacob

1971

Metabolic Turnover in the Nervous System

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Détermination de la fraction du génome codant pour les RNA ribosomiques et messagers dans le cerveau du rat adulte

Cited by 37 publications

References 9 publications

Quantitative versus qualitative RNA and protein changes in the brain during behavior.

Quantitative versus qualitative RNA and protein changes in the brain during behavior.

Messenger RNA in avian erythroblasts at the transcriptional and translational levels and the problem of regulation in animal cells

RNA Metabolism

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