Determination of 44 major components and chemical profiling of saccharide in Chinese medicinal formula Lanqin Oral Liquid

Abstract

Introduction: Lanqin Oral Liquid (LQL) is a traditional Chinese medicine preparation (TCMP) containing five herbal medicines and has been commonly used for the treatment of pharyngitis and hand-foot-and-mouth disease in clinic. The material basis of LQL has been reported in our previous study, but the contents of the major components and the features of saccharide in LQL are still unclear.Objectives: This study aimed to establish accurate and rapid methods for the quantification of the major components and profiling of saccharide in LQL. The quantitative results combined with similarity evaluation were applied to improve the quality control of LQL.Methodology: An ultra-high performance liquid chromatography coupled with triplequadrupole tandem mass spectrometry (UPLC-QQQ-MS) method was utilised to determine 44 major components. Cosine similarity was used to evaluate the similarities among 20 batches of LQL based on the quantitative results of 44 major components. The physicochemical properties, structure, composition, and contents of saccharide in LQL were detected by a combination of chemical and instrumental analysis.Results: A total of 44 compounds, including flavonoids, iridoid glycosides, alkaloids, and nucleosides, were accurately determined. The 20 batches of LQL were remarkably similar (> 0.95). In addition, D-glucose, galactose, D-glucuronic acid, arabinose, and D-mannose were detected in saccharide of LQL. The contents of saccharide in LQL were 13.52-21.09 mg/ml. Conclusions:The established methods can be applied for the comprehensive quality control of LQL, including characterisation of saccharide and quantification of representative components. Our study will provide a robust chemical foundation for disclosing the quality markers of its therapeutic effect.

Systematic Screening of the Chemical Constituents of Lanqin Oral Liquid by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

“…Flavonoids, for example, baicalein, baicalin, wogonin, wogonoside, oroxylin A, and scutellarin, show Compounds including flavonoids, alkaloids, terpenoids, organic acids, and phenylpropanoids were identified in Lanqin oral liquid. The content of compounds, such as baicalin, wogonoside, baicalein, wogonin, phellodendrine, magnoflorine, berberine, palmatine, indirubin, genipin-1-O-β-D-gentiobioside, geniposide, obaculactone, obacunone, and chlorogenic acid, were determined in Lanqin oral liquid [28]. Based on the combined content and pharmacological activity studies of the compounds, these chemical constituents with high content and pharmacological activity could be the pharmacodynamic substances that play an important role in vivo.…”

Optimization of the Zhou Tian Formula extraction technology based on AHP‐CRITIC method and analysis of transfer efficiency of key components based on HPLC fingerprinting

“…For instance, Bao analyzed the active ingredients in Sangbaipi decoction, 18 and Zhao et al conducted qualitative and quantitative analyses of representative components in Lanqin oral liquid. 19 However, few researchers have studied the entire process, from the intermediate to the final product. Herein, we conducted a study of water decoction, extract powder, and granules, with the aim of exploring the principles governing the quality of transfer during the conversion of water decoction to extract powder and subsequently to granules.…”