Determination of albumin sorption to intraocular lenses by radiolabeling and confocal laser scanning microscopy

Luensmann, Doerte; Heynen, Miriam; Liu, Lina; Sheardown, Heather; Jones, Lyndon

doi:10.1016/j.jcrs.2009.05.052

Cited by 6 publications

(6 citation statements)

References 52 publications

(54 reference statements)

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“…The above findings are in accordance with a report that serum albumin sorption is limited to the surface region of hydrophobic IOLs but penetrates deep into hydrophilic acrylic IOLs [8]. Other researchers have also reported that although hydrophobic IOLs develop and bind adhesive molecules such as vitronectin and collagen type IV less than hydrophilic IOLs, hydrophobic IOLs showed much more fibronectin binding on their surface than hydrophilic IOLs [9].…”

Section: Discussionsupporting

confidence: 92%

Cell Adhesion and Glistening Formation in Hybrid Copolymer Intraocular Lenses

Fujita¹,

Tanaka

Miyata

et al. 2012

Ophthalmic Res

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The purpose of this study is to investigate the cell adhesion and glistening formation properties of various foldable intraocular lenses (IOLs) in vitro. Three conventional hydrophobic methacrylate acrylic (MA) IOLs, a hydrophilic hydroxyethyl methacrylate (HEMA) IOL and a hybrid MA/HEMA copolymer IOL were investigated for immunologically activated cell adhesion and for formation of glistenings resulting from cavitation, by analysis of digital images using NIH Image J PC software. The MA IOLs exhibited a low level of adhering cells but a high level of glistening formation, the HEMA IOL exhibited the reverse tendency, and the MA/HEMA IOL exhibited a low level of both, thus indicating that hybrid MA/HEMA IOLs are less susceptible than HEMA IOLs to cell adhesion and less susceptible than MA IOLs to glistening formation.

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Section: Discussionsupporting

confidence: 92%

Cell Adhesion and Glistening Formation in Hybrid Copolymer Intraocular Lenses

Fujita¹,

Tanaka

Miyata

et al. 2012

Ophthalmic Res

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“…However, due to the attachment of a probe, conformational changes can occur, that may expose interior lysines to the outside which may then allow more dye molecules to attach. In the case of BSA and LY, we determined in a previous study a change of ∼5% in size and charge for BSA, comparing conjugated with unconjugated BSA over the incubation time of up to 14 days 66. These findings, and the fact that the DOL was determined to be <3, confirmed that the BSA remained in an acceptable conformational state throughout the experiment when conjugated with LY.…”

Section: Discussionsupporting

confidence: 56%

“…In the case of BSA and LY, we determined in a previous study a change of $5% in size and charge for BSA, comparing conjugated with unconjugated BSA over the incubation time of up to 14 days. 66 These findings, and the fact that the DOL was determined to be <3, confirmed that the BSA remained in an acceptable conformational state throughout the experiment when conjugated with LY. The native structure of both human and bovine serum albumin has 35 cysteines, of which 34 are typically paired with disulfide bonds to cystines and the sulphydryl group of the single Cys-34 remains mainly unbound.…”

Section: Discussionmentioning

confidence: 63%

Impact of fluorescent probes on albumin sorption profiles to ophthalmic biomaterials

Luensmann

Jones

2010

J Biomed Mater Res

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Bovine serum albumin (BSA) was conjugated to three organic fluorescent probes, 5-(4,6-dichloro-s-triazin-2-ylamino)fluorescein hydrochloride (DTAF), Rhodamine B isothiocyanate (RITC), and Lucifer yellow VS (LY). The protein sorption profile to one pHEMA-based (etafilcon A) and three silicone hydrogel (SH) contact lens types (lotrafilcon B, balafilcon A and senofilcon A) was determined using confocal laser scanning microscopy. In addition, all lenses were incubated in dye solutions containing the fluorescent probe alone; and in a separate experiment BSA accumulation was quantified using radiolabeling. The different fluorescent conjugates showed similar sorption profiles for the pHEMA-based lens, but marked differences for all SH lenses. Lotrafilcon B accumulated more protein on the surface as compared to the matrix, independent of the fluorescent probe used for conjugation. Protein sorption varied for senofilcon A, with DTAF-BSA sorbing primarily to the surface region, while the other conjugates penetrated in equal amounts into the matrix. Balafilcon A exhibited smaller differences between conjugates, with LY-BSA allowing the protein to fully penetrate the matrix, while the other conjugates showed minor surface adsorption. Sorption curves of unbound dyes were often similar compared to the conjugated results. BSA profiles to pHEMA-based and silicone hydrogel lenses were highly dependent on the fluorescent probe used and none of the probes accurately reflected quantitative protein levels for the lens materials investigated.

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“…In the case of the RGD-adsorbed sample, the high fluorescence may have resulted from the sorption phenomenon (i.e. molecules penetrated into the inner space of the hydrophilic acrylic polymer) [87] . Although the extreme condition of autoclaving had been applied to remove the surface-adsorbed peptides, a large amount of FITC-RGD peptides was “sorbed” inside the polymer, which could explain the low cell coverage of the FITC-RGD-adsorbed surface.…”

Section: Discussionmentioning

confidence: 99%

RGD Surface Functionalization of the Hydrophilic Acrylic Intraocular Lens Material to Control Posterior Capsular Opacification

et al. 2014

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Posterior Capsular Opacification (PCO) is the capsule fibrosis developed on implanted IntraOcular Lens (IOL) by the de-differentiation of Lens Epithelial Cells (LECs) undergoing Epithelial Mesenchymal Transition (EMT). Literature has shown that the incidence of PCO is multifactorial including the patient's age or disease, surgical technique, and IOL design and material. Reports comparing hydrophilic and hydrophobic acrylic IOLs have shown that the former has more severe PCO. On the other hand, we have previously demonstrated that the adhesion of LECs is favored on hydrophobic compared to hydrophilic materials. By combining these two facts and contemporary knowledge in PCO development via the EMT pathway, we propose a biomimetically inspired strategy to promote LEC adhesion without de-differentiation to reduce the risk of PCO development. By surface grafting of a cell adhesion molecule (RGD peptide) onto the conventional hydrophilic acrylic IOL material, the surface-functionalized IOL can be used to reconstitute a capsule-LEC-IOL sandwich structure, which has been considered to prevent PCO formation in literature. Our results show that the innovative biomaterial improves LEC adhesion, while also exhibiting similar optical (light transmittance, optical bench) and mechanical (haptic compression force, IOL injection force) properties compared to the starting material. In addition, compared to the hydrophobic IOL material, our bioactive biomaterial exhibits similar abilities in LEC adhesion, morphology maintenance, and EMT biomarker expression, which is the crucial pathway to induce PCO. The in vitro assays suggest that this biomaterial has the potential to reduce the risk factor of PCO development.

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Determination of albumin sorption to intraocular lenses by radiolabeling and confocal laser scanning microscopy

Cited by 6 publications

References 52 publications

Cell Adhesion and Glistening Formation in Hybrid Copolymer Intraocular Lenses

Cell Adhesion and Glistening Formation in Hybrid Copolymer Intraocular Lenses

Impact of fluorescent probes on albumin sorption profiles to ophthalmic biomaterials

RGD Surface Functionalization of the Hydrophilic Acrylic Intraocular Lens Material to Control Posterior Capsular Opacification

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