Determination of aminoglycoside antibiotics by reversed-phase ion-pair high-performance liquid chromatography coupled with pulsed amperometry and ion spray mass spectrometry

Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in serum

“…NPLC is not compatible with the electrospray process. Some ion-pair chromatography methods with volatile reagents and MS detection for the determination of gentamicin, streptomycin, neomycin or tobramycin were described [16][17][18][19][20]. HILIC shows separation similar to NPLC but it is possible to use water and volatile buffering agents, which are compatible for mass spectrometry.…”

“…A LC-MS method for the determination of kanamycin using phenylisocyanate as a derivatisation reagent was described [15]. The determinations of aminoglycosides in animal tissues, of gentamicin in wastewater and of tobramycin in serum by ion pair chromatography with volatile reagents and MS detection were reported in literature [16][17][18][19][20]. Normal-phase liquid chromatography (NPLC) ought to be suitable for the separation of strong hydrophilic compounds.…”

Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC–MS/MS

“…This detection technique is highly selective and specific, and therefore permits the use of short run times and minimal sample clean-up procedures. Mass spectrometric detection (MS) [16,17] and tandem mass spectrometric detection (MS/MS) [18][19][20][21][22] of aminoglycosides in biological matrices has been described previously, two of these focussing on tobramycin [18,22].…”

High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization

“…1). The analysis of aminoglycosides are usually performed using pre-column [2][3][4][5][6][7][8] and post-column derivatization [9] methods, or other detections such as evaporative light scattering detection [10], pulsed electrochemical detection [11][12][13] and mass spectrometry [12,14,15]. Zhou et al [16] had reported an analytical method for veritlmicin in plasma and 1-fluoro-2,4-dinitrobenzene was used for derivatization.…”