Determination of Ascorbic Acid in Pharmaceutical Dosage Forms Based on Oxidation at the Tubular Carbon Electrode

Mason, William D.; Gardner, Thomas; Stewart, James T.

doi:10.1002/jps.2600610827

Cited by 13 publications

(2 citation statements)

References 4 publications

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“…Several methods have been developed for the analysis of this vitamin, including electrochemical [1], spectrophotometric [2], catalytic [3], spectrofluorimetric and chromatographic methods. Spectrofluorimetry and microfluorimetry [4] are based on the reaction of DHAA with o-phenylenediamine ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Comparison of high-performance liquid chromatography and spectrofluorimetry for vitamin C analysis of green beans (Phaseolus vulgaris L.)

Sánchez‐Mata

Cámara

Díez-Marqués

et al. 2000

Eur Food Res Technol

131

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A spectrofluorimetric method and a chromatographic method [high-performance liquid chromatography -ultraviolet (HPLC-UV) detection] were compared for vitamin C analysis of green beans. For HPLC-UV, the determination was performed before [ascorbic acid (AA)] and after reduction with dithiothreitol [AA c dehydroascorbic acid (DHAA)]; for spectrofluorimetry, DHAA was determined after oxidation with activated charcoal. The fluorimetric determination of DHAA before oxidation was also carried out, and showed good precision but poor accuracy (very high recovery percentages). HPLC-UV showed better linearity and sensitivity, while spectrofluorimetry was more precise. Both methods were suitable for green bean samples, and the choice of which one to use depends on the particular interest of the analysis: spectrofluorimetry may be preferable when the total content of vitamin C is required, but not for individual analysis of each form. HPLC-UV is more suitable when the interest of the analysis is the ascorbic acid form as a deterioration index of vegetable products.

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Section: Introductionmentioning

confidence: 99%

Comparison of high-performance liquid chromatography and spectrofluorimetry for vitamin C analysis of green beans (Phaseolus vulgaris L.)

Sánchez‐Mata

Cámara

Díez-Marqués

et al. 2000

Eur Food Res Technol

131

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“…These steps are drawbacks of these methods, not only because of the time spent in this stage, which increases the chance of AA oxidation, but also because of the cost of equipment required. On the other hand, different electroanalytical methods have been proposed for the determination of AA, several of which avoid some laborious steps when preparing the sample (Mason et al, 1972;Khristova et al, 1976;Matsumoto et al, 1981;Falat and Cheng, 1982;Nasser et al, 1987;Park and Shaw, 1989;Lyons et al, 1991;Campiglio, 1993). However, many of these are based on the use of membrane electrodes sensitive to a particular compound, which is the product of reaction between AA and the reagent used as the titrant.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Iodometric Titration of Ascorbic Acid with Dead-Stop End-Point Detection in Fresh Vegetables and Fruit Samples

Verdini

Lagier

2000

J. Agric. Food Chem.

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The present work describes a method for determining ascorbic acid, which combines iodometry with a voltammetric technique to detect the end point of the titration. In addition, the validity of the method applied to natural vegetable or fruit samples was assessed. The results were compared with those obtained by an accurate method such as HPLC using UV detection. Similar values of ascorbic acid for different natural samples were obtained by means of this approach (p > 0.05). The limit of quantification was 0.1 mg. This technique presents the advantage of other electroanalytical methods such as avoiding filtration or ultracentrifugation steps, with the additional benefit of using the platinum electrodes, which are routinely used in the laboratory. These facts allow a rapid and efficient quantification of ascorbic acid with very low cost of reagents and equipment.

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Determination of Ascorbic Acid in Pharmaceutical Dosage Forms Based on Oxidation at the Tubular Carbon Electrode

Cited by 13 publications

References 4 publications

Comparison of high-performance liquid chromatography and spectrofluorimetry for vitamin C analysis of green beans (Phaseolus vulgaris L.)

Comparison of high-performance liquid chromatography and spectrofluorimetry for vitamin C analysis of green beans (Phaseolus vulgaris L.)

Voltammetric Iodometric Titration of Ascorbic Acid with Dead-Stop End-Point Detection in Fresh Vegetables and Fruit Samples

Determination of Dopa in Pharmaceutical Dosage Forms Based on Oxidation at Tubular Carbon Electrode

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