Determination of carbon and nitrogen in microbial biomass of southern-Taiga soils by different methods

Макаров, М. И.; Малышева, Т. И.; Maslov, M. N.; Kuznetsova, E. Yu.; Menyailo, O. V.

doi:10.1134/s1064229316060053

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…Another observation is that in the control, nonirradiated samples, the highest numbers of bacteria and fungi colonies formed in the inoculation from soil 3, which had the highest extracted carbon content among all the soils after an irradiation of 25 KGy, although it did not have the highest carbon concentration (Table 2). This confirms that other factors besides the organic matter content affect the reproduction capacity of soil microorganisms, and it reinforces the current opinion that microbial biomass is a useful indicator of soil quality under different management regimes (Jannoura et al, 2013;Makarov et al, 2016). This peculiar behavior of soil 3 may reflect better conditions for microbial growth in its collection area, which are produced by the combination of high soil mobilization (maize cultivation) associated with the application of manure and litter inputs and the favorable microclimate due to the presence of the trees in the agroforestry system.…”

Section: Resultssupporting

confidence: 86%

“…Among soil quality indicators, microbial biomass is one of the best (Brookes et al, 2008;Jannoura, Bruns, & Joergensen, 2013;Makarov, Malyesheva, Maslov, Kuznetsovaa, & Menyailo, 2016). Soil microbial biomass (SMB), excluding plant roots and animals larger than 5 μm³, corresponds with, on average, 2 to 5% of soil organic matter (Vance, Brooks, & Jenkinson, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…Soil microbial biomass (SMB), excluding plant roots and animals larger than 5 μm³, corresponds with, on average, 2 to 5% of soil organic matter (Vance, Brooks, & Jenkinson, 1987). There is no consensus on the best method to quantify SMB under diverse edapho-ecological conditions, either because the results have not been very reliable, or the procedures are too labor intensive or too expensive (Hartmann, Frey, Mayer, Mäder, & Widmer, 2015;Makarov, 2016). Thus, it is advisable to use more than one method for SMB quantification.…”

Section: Introductionmentioning

confidence: 99%

“…Comparisons of some of the different methods have been made, but they seldom verify the effectiveness of the methods in killing soil microbiota (Makarov et al, 2016). Chloroform fumigation does not kill all microorganisms, and the method is usually limited by the need for a large set of desiccators (Wolf, Dao, Scott, & Lavy, 1989).…”

Section: Introductionmentioning

confidence: 99%

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<b>Comparison of methods to quantify soil microbial biomass carbon

Moura¹,

Carrido

Sousa

et al. 2018

Acta Sci. Agron.

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Soil microorganism biomass is an important soil quality indicator. The microbial biomass of soil was determined by killing and lysing the soil microbes by fumigation with chloroform, irradiation with gamma rays, or irradiation with microwaves. Four soils with increasing carbon concentrations (5, 10, 15, and 30 g kg-1) were analyzed using four methods: the direct application of chloroform, chloroform fumigation, microwave irradiation, and gamma ray irradiation with radiation doses of 15, 25, 35, 45, and 60 KGy. The fungi and bacteria in the soil were quantified by plate counting. Microwave irradiation and gamma irradiation with doses equal to or above 25 KGy killed all the soil microorganisms, but the chloroform methods did not. The carbon liberation increased with higher gamma doses, while the microbe mortality rates were the same, indicating that carbon was liberated from organic matter sources other than microorganisms. The biomasses determined by the microwave method correlated with those determined by the fumigation and 25 KGy gamma irradiation methods, but their values differed among all methods for at least one soil type. Despite this discrepancy, all methods were consistent in ranking microbial biomasses in increasing order of soil carbon concentrations, which corresponds with decreasing land use intensities.

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<b>Comparison of methods to quantify soil microbial biomass carbon

Moura¹,

Carrido

Sousa

et al. 2018

Acta Sci. Agron.

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“…Wheat was sprayed at 15 day intervals in the recommended and increased dosages. The soil samples were taken 10 days after spraying.The MB by carbon was determined by the method of rehydration[Makarov et al 2016]. The urease activity was determined by the method of Galstyan in Khaziyev's modification on a spectrophotometer (HACH-LANGE, Germany)[Khaziev 2005].…”

mentioning

confidence: 99%

Potential Attractiveness of Soil Fungus Trichoderma Inhamatum for Biodegradation of the Glyphosate Herbicide

Kunanbayev¹,

Churkinа²,

Rukavitsina³

et al. 2019

J. Ecol. Eng.

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The T. inhamatum F6-2014 fungus was isolated from the black soils of Northern Kazakhstan in stationary experiments with many years of herbicide use. The research was aimed at studying the biodegrading activity of T. inhamatum in relation to an herbicide with the glyphosate active ingredient, both in vitro and in situ. Under the laboratory conditions, the highest growth rate of a colony of T. inhamatum was observed in the reference variant, and reached 49.0 ± 2.5 mm. During the field experiments, a significant increase was noted in the microbial biomass (MB) on the first day in the herbicidal variants and ranged from 239.2 to 322.8 µg/g of soil. On the 15th day, the level of MB in all herbicide variants increased 1.6-2.1 times, and in the reference-2.3 times. By the second month, the level of MB reduced 1.5-2.0 times in all variants. By the third month of the studies, the greatest MB decrease was noted in the reference-82.8 µg/g. The level of urease activity during the first day in all variants, including the reference, ranged between 0.12-0.15 mg/g of soil. On the 15th day, a significant (3.3 times) increase in the urease activity was noted in the variants with glyphosate, compared to the reference. Subsequently, the difference in the level of enzyme activity was insignificant between the variants. The MB and the urease activity in the first fifteen days increase due to the more active decomposition of glyphosate by strain.

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