Determination of choline in soybean meal by liquid chromatography with the ion-exchange membrane detector

Dorsey, John G.; Hansen, Larry C.; Gilbert, T. W.

doi:10.1021/jf60227a027

J. Agric. Food Chem.

1980

DOI: 10.1021/jf60227a027

|View full text |Cite

Determination of choline in soybean meal by liquid chromatography with the ion-exchange membrane detector

John G. Dorsey¹,

Larry C. Hansen²,

T. W. Gilbert³

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1987

2024

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 5 publications

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

The determination of choline in vitamin preparations, infant formula and selected foods by capillary zone electrophoresis with indirect ultraviolet detection

Carter

Trenerry

1996

Electrophoresis

View full text Add to dashboard Cite

A method for the determination of choline in vitamin preparations, infant formula and selected foods by capillary zone electrophoresis with indirect ultra-violet detection at 214 nm is described. the "free" choline in the vitamin preparations was simply extracted with water, the solutions filtered and analysed. The "free" and"bound" choline in the other products was extracted with methanol. The near dry extract was then treated with hot saturated aqueous calcium hydroxide to hydrolyse the "bound" choline before analysis. A 75 cm x 75 microns uncoated fused silica capillary column with an electrolyte consisting of 5 mM 1-methylimidazole, pH 4.5, and an operating voltage of 30 kV was used for all of the determinations. Using these conditions, choline migrates in less than 3 min and is well separated from the other components in the electropherograms. The tetramethylammonium ion was used as the internal standard. The instrument repeatability data for area calculation and migration time variation were good (coefficient of variation, CV, area calculation 1.4%, n = 10; CV for migration time variation 1.0%, n = 20). The level of detection for choline using this procedure is 5 mg/100 g.

show abstract

The determination of choline in vitamin preparations, infant formula and selected foods by capillary zone electrophoresis with indirect ultraviolet detection

Carter

Trenerry

1996

Electrophoresis

View full text Add to dashboard Cite

show abstract

Analytical approaches to determination of total choline in foods and dietary supplements

Phillips

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

Choline is a quaternary amine that is synthesized in the body or consumed through the diet. Choline is critical for cell membrane structure and function and in synthesis of the neurotransmitter acetylcholine. Although the human body produces this micronutrient, dietary supplementation of choline is necessary for good health. The major challenge in the analysis of choline in foods and dietary supplements is in the extraction and/or hydrolysis approach. In many products, choline is present as choline esters, which can be quantitated individually or treated with acid, base, or enzymes in order to release choline ions for analysis. A critical review of approaches based on extraction and quantitation of each choline ester as well as hydrolysis-based methods for determination of total choline in foods and dietary supplements is presented.

show abstract

Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods

Hirakawa,

Fujita,

Katayama

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Determining the content of the nutrient choline in foods and obtaining the required amount from the diet are crucial. One way to measure choline in foods is by converting choline esters to free choline via acid hydrolysis, followed by quantifying the total choline, as adopted by the AOAC method (AOAC-Choline); however, certain choline esters are difficult to hydrolyse. Here, we investigated various acid hydrolysis conditions to establish a reliable method for determining the total choline in foods by detecting free choline using highly sensitive and selective mass spectrometry. Hydrolysis in 0.055 mol/L HCl for 8 h in an autoclave (121 °C) was found to be optimal for the hydrolysis of choline esters in various foods. Twenty-four foods, including grains, seed, vegetables, fruits, mushroom, algae, fish, meats, beverage, processed foods, and egg, were measured. The trends in the total choline content were consistent with previous reports; however, the choline content was 10–20% higher than that measured using AOAC-Choline. Therefore, re-evaluation of the total choline content in foods using our constructed method is recommended. This reassessment will allow for a more reliable determination of choline intake for maintaining health.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Determination of choline in soybean meal by liquid chromatography with the ion-exchange membrane detector

Cited by 5 publications

References 14 publications

The determination of choline in vitamin preparations, infant formula and selected foods by capillary zone electrophoresis with indirect ultraviolet detection

The determination of choline in vitamin preparations, infant formula and selected foods by capillary zone electrophoresis with indirect ultraviolet detection

Analytical approaches to determination of total choline in foods and dietary supplements

Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods

Contact Info

Product

Resources

About